本文選題：正畸金屬絲 + 焊接��； 參考：《吉林大學(xué)》2014年碩士論文

【摘要】：隨著材料加工工藝的發(fā)展，鎳鈦-不銹鋼異種金屬弓絲的激光焊接方法不斷革新，目前中間加層焊接方法較普遍，其中間加層金屬有純鎳、鈷、銅。與以往激光焊接金屬弓絲相比加銅中間層激光焊接的鎳鈦-不銹鋼金屬弓絲具有焊接處光滑平整、抗拉強(qiáng)度大、鎳鈦弓絲側(cè)變形恢復(fù)率高、接頭彎曲角大等優(yōu)點(diǎn)。該項(xiàng)發(fā)明已申請(qǐng)國(guó)家專利，相關(guān)研究已檢測(cè)該復(fù)合弓絲的機(jī)械性能、腐蝕性能及離子析出，然而針對(duì)該復(fù)合弓絲的生物安全性檢測(cè)尚未報(bào)道，本文首先檢測(cè)其細(xì)胞安全性。為該復(fù)合弓絲的臨床應(yīng)用提供生物安全依據(jù)。 目的：檢測(cè)鎳鈦-不銹鋼激光焊復(fù)合弓絲的體外細(xì)胞毒性，以及其細(xì)胞毒性與時(shí)間和濃度的相關(guān)性，并且與臨床常用不銹鋼弓絲、鎳鈦弓絲比較，探討復(fù)合弓絲的細(xì)胞安全性。 方法：實(shí)驗(yàn)分為兩部分。實(shí)驗(yàn)一：將鎳鈦-不銹鋼復(fù)合弓絲經(jīng)95%酒精浸泡、超聲蕩洗、去離子水沖洗、烘干、高溫高壓滅菌后浸泡于高糖細(xì)胞培養(yǎng)液（Dulbecco’s modified Eagle’s medium，DMEM），含10%血清與1%雙抗（青霉素100000U/L和鏈霉素100000μg/L），浸泡42h。將浸提液配置成體積分?jǐn)?shù)為50%、40%、30%、20%、10%的稀釋液（n=6），與鋪板48h的小鼠成纖維細(xì)胞系L929細(xì)胞共培養(yǎng)，采用甲基噻唑基四唑（methyl thiazolyl tetrazolium，MTT）檢測(cè)培養(yǎng)24h、48h后各稀釋液對(duì)小鼠成纖維細(xì)胞L929的細(xì)胞毒性。實(shí)驗(yàn)二：將復(fù)合弓絲組與不銹鋼弓絲組、鎳鈦弓絲組（二者處理方式同實(shí)驗(yàn)一中復(fù)合弓絲）浸泡于高糖細(xì)胞培養(yǎng)液（含10%血清與1%雙抗）42h。將浸提液配置成體積分?jǐn)?shù)為100%、75%、50%、25%的稀釋液(n=6)，與鋪板48h的小鼠成纖維細(xì)胞系L929細(xì)胞共培養(yǎng)，采用MTT檢測(cè)并比較48h后各稀釋液對(duì)L929的細(xì)胞毒性。陰性對(duì)照組加入高密度聚乙烯浸提液（100%體積分?jǐn)?shù)），陽(yáng)性對(duì)照組加入二甲基亞砜。 結(jié)果：各實(shí)驗(yàn)組浸提液細(xì)胞毒性均為0～1級(jí)，陰性對(duì)照組細(xì)胞毒性為0級(jí)，，陽(yáng)性對(duì)照組細(xì)胞毒性為4級(jí)。實(shí)驗(yàn)一：24h與48h共培養(yǎng)結(jié)果顯示隨著50%至10%復(fù)合弓絲浸提液體積分?jǐn)?shù)的降低，光密度值（optical density，OD）呈顯著增高趨勢(shì)；除20%復(fù)合弓絲浸提液外，相同體積分?jǐn)?shù)復(fù)合弓絲浸提液與L929共培養(yǎng)48h后光密度值均顯著低于共培養(yǎng)24h。實(shí)驗(yàn)二：100%、75%、50%、25%復(fù)合弓絲組浸提液OD值（1.964±0.122、2.084±0.056、2.056±0.071、2.096±0.050）均顯著低于與不銹鋼組（2.168±0.091、2.227±0.160、2.302±0.052、2.301±0.060）、鎳鈦弓絲組浸提液（2.138±0.105、2.262±0.050、2.271±0.082、2.294±0.056）（P0.05）。而相同體積分?jǐn)?shù)的不銹鋼組與鎳鈦弓絲組的吸光度值無(wú)統(tǒng)計(jì)學(xué)差異。 結(jié)論：鎳鈦-不銹鋼激光焊復(fù)合弓絲的細(xì)胞毒性作用隨著浸提液體積分?jǐn)?shù)降低呈現(xiàn)降低；復(fù)合弓絲的細(xì)胞毒性作用隨著培養(yǎng)時(shí)間的延長(zhǎng)呈略增高趨勢(shì)；復(fù)合弓絲的細(xì)胞毒性作用較不銹鋼弓絲、鎳鈦弓絲明顯。綜上所述，該復(fù)合弓絲的細(xì)胞毒性作用較小，在材料毒性反應(yīng)安全級(jí)別范圍內(nèi)。
[Abstract]:With the development of material processing technology, the laser welding method of nickel-titanium stainless steel dissimilar metal arch wire is innovated continuously. At present, the welding method of interlayer welding is relatively common, and the interlayer metals include pure nickel, cobalt and copper. Compared with the previous laser welding of metal bow wire, the Ni-Ti stainless steel arc wire with copper interlayer laser welding has the advantages of smooth welding place, high tensile strength, high recovery rate of deformation on the side of Ni-Ti bow wire, and large bending angle of joint, etc. The invention has been applied for a national patent. The mechanical properties, corrosion performance and ion precipitation of the composite bow wire have been examined by relevant research. However, the biosafety detection of the composite bow wire has not been reported. In this paper, the cell safety of the composite bow wire has been first tested. To provide the biosafety basis for the clinical application of the composite arch wire. Objective: to detect the cytotoxicity of Ni-Ti stainless steel laser welded composite arch wire in vitro and its correlation with time and concentration, and to investigate the cell safety of composite wire compared with stainless steel arch wire and nickel titanium arch wire commonly used in clinic. Methods: the experiment was divided into two parts. Experiment 1: the Ni-Ti stainless steel composite bow wire was soaked in 95% alcohol, washed by ultrasonic swing, washed with deionized water, and dried. After sterilizing under high temperature and high pressure, the cells were immersed in high glucose cell culture medium (Dulbeccos modified Eagleus medium DMEM), containing 10% serum and 1% double antibody (penicillin 100000U / L and streptomycin 100000 渭 g / L) for 42 h. The extractant was co-cultured with the mouse fibroblast cell line L929 for 48 hours. The cytotoxicity of each diluent to L929 was detected by (methyl thiazolyl tetrazoliumMTT for 24 h or 48 h. Experiment 2: the composite wire group, stainless steel arch wire group and nickel titanium arch wire group (both treated in the same way as in experiment 1) were immersed in high glucose cell culture medium (containing 10% serum and 1% double antibody) for 42 h. The extract was co-cultured with the mouse fibroblast cell line L929 for 48 hours. The cytotoxicity of the diluents to L929 was measured by MTT assay and compared after 48 hours. The negative control group added high density polyethylene extract (100% volume fraction), the positive control group added dimethyl sulfoxide. Results: the cytotoxicity of the extract in each experimental group was 0 grade 1, that in the negative control group was 0 grade, and that in the positive control group was grade 4. The results of experiment 1: 24 h and 48 h coculture showed that the optical density OD increased significantly with the decrease of 50% ~ 10% composite wire extraction liquid integral, except 20% compound bow wire extraction solution. The optical density was significantly lower than that of co-cultured for 24 h after co-culture of the same volume fraction of the compound arch wire extract and L929 for 48 h. In experiment 2, the OD value of the extract in the 25% composite wire group (1.964 鹵0.122) was significantly lower than that in the stainless steel group (2.168 鹵0.091 鹵2.227 鹵0.160 鹵2.302 鹵0.0522.301 鹵0.060) and the nickel titanium wire group (2.138 鹵0.105 鹵2.262 鹵0.0502.271 鹵0.0822.294 鹵0.056) (P 0.05), and was significantly lower than that in the stainless steel group (2.168 鹵0.091) and the nickel titanium wire group (2.138 鹵0.105) 2.262 鹵0.0502.271 鹵0.0822.294 鹵0.056 (P0.05). However, there was no significant difference in absorbance between stainless steel group and nickel titanium arch wire group. Conclusion: the cytotoxicity of Ni-Ti stainless steel laser welded composite wire decreased with the decrease of the number of extracted liquid, and the cytotoxicity of composite wire increased slightly with the prolongation of culture time. The cytotoxicity of composite arch wire was more obvious than that of stainless steel arch wire and nickel titanium arch wire. To sum up, the cytotoxicity of the composite arch wire is relatively small, and the safety level of the material toxicity reaction is within the range.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 李明高;孫大謙;邱小明;孫德新;殷世強(qiáng);;TiNi形狀記憶合金連接技術(shù)的研究進(jìn)展[J];材料導(dǎo)報(bào);2006年02期

2 李明高,邱小明,孫大千,劉衛(wèi)紅;TiNi形狀記憶合金與不銹鋼焊接接頭性能比較[J];焊接;2005年03期

3 李明高,孫大謙,邱小明,劉衛(wèi)紅;TiNi形狀記憶合金與不銹鋼激光釬焊AgCuZnSn釬料的研制[J];焊接學(xué)報(bào);2005年07期

4 邱小明,李明高,孫大千,劉衛(wèi)紅;TiNi形狀記憶合金與不銹鋼釬焊接頭的微觀組織與性能[J];機(jī)械工程學(xué)報(bào);2005年02期

5 馮翠娟;張雪;王強(qiáng);張揚(yáng);秦科;;表面氧化鎳鈦合金對(duì)L-929細(xì)胞凋亡、總RNA量及caspase-3,8,9 mRNA表達(dá)影響的研究[J];口腔醫(yī)學(xué);2011年01期

6 李明高,邱小明,孫大千,劉衛(wèi)紅;TiNi形狀記憶合金與不銹鋼的儲(chǔ)能焊[J];輕金屬;2005年07期

相關(guān)博士學(xué)位論文 前1條

1 李洪梅;TiNi形狀記憶合金與不銹鋼異種材料激光焊研究[D];吉林大學(xué);2011年

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