本文選題：聚合物涂層 + 人類脂肪干細(xì)胞　； 參考：《高等學(xué)�；瘜W(xué)學(xué)報》2017年10期

【摘要】：采用紫外固化法制備了基于丙烯酸酯類水凝膠的聚合物涂層(PC),并用X射線光電子能譜(XPS)、水接觸角(WCA)和原子力顯微鏡(AFM)分別對PC進(jìn)行了化學(xué)組成和表面性能的表征.在PC表面進(jìn)行了人類脂肪干細(xì)胞(h ASC)的體外長期培養(yǎng)擴(kuò)增,得到的第3代細(xì)胞的生物學(xué)表征結(jié)果表明,干細(xì)胞在PC表面能正常黏附生長,流式細(xì)胞儀檢測發(fā)現(xiàn)干細(xì)胞對特征標(biāo)記物CD49d,CD73,CD105的陽性顯性比例較高,對HLA-DR和CD31幾乎不顯性,說明擴(kuò)增的干細(xì)胞具有h ASC特征.對PC上擴(kuò)增的干細(xì)胞進(jìn)行誘導(dǎo)分化,并用油紅O、茜素紅和阿利新藍(lán)分別進(jìn)行染色分析,結(jié)果表明,該干細(xì)胞保留了h ASC的多能特性:能分化為成脂、成骨和成軟骨細(xì)胞.含有單體甲基丙烯酰氧乙基三甲基氯化銨(DMC)、甲基丙烯酸環(huán)己酯(CHMA)和甲基丙烯酸-2-(二乙氨基)乙酯(DEAEMA)的PC2(質(zhì)量比為3∶1∶2)在用于h ASC體外長期培養(yǎng)時,比其它PC和TCP更有利于細(xì)胞的黏附和增殖,純化細(xì)胞,保持其多能性.實時熒光定量PCR(RT-q PCR)的分析表明PC2上得到的細(xì)胞更容易向成骨和成軟骨細(xì)胞分化.
[Abstract]:Polymer coatings (PC) based on acrylate hydrogels were prepared by UV curing method. The chemical composition and surface properties of PC were characterized by X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) and atomic force microscope (AFM). Human adipose stem cells (hASC) were cultured and amplified on PC surface for a long time in vitro. The results of biological characterization of the third passage cells showed that the stem cells could grow normally on the PC surface. The results of flow cytometry showed that the stem cells had a high percentage of positive dominance to CD49dU CD73 and CD105, but almost no dominance to HLA-DR and CD31, indicating that the expanded stem cells had hASC characteristics. The stem cells amplified on PC were induced to differentiate and stained with oil red O, alizarin red and alisin blue respectively. The results showed that the stem cells retained the pluripotent characteristics of hASC: they could differentiate into fat-forming, osteogenic and chondrogenic cells. PC2 containing monomers methacryloxy ethyl trimethylammonium chloride (DMC), cyclohexyl methacrylate (CHMA) and diethylaminoethyl methacrylate (DEAEMA) (with a mass ratio of 3:1:2) was used in long-term culture of hASC in vitro. Compared with other PC and TCP, it is more favorable to cell adhesion and proliferation, purify cells and maintain their pluripotency. Real-time fluorescent quantitative PCR (RT-q PCR) analysis showed that PC2 cells were more likely to differentiate into osteoblasts and chondroblasts.
【作者單位】： 常州大學(xué)材料科學(xué)與工程學(xué)院;常州大學(xué)生物醫(yī)學(xué)工程與健康科學(xué)研究院;江蘇和澤生物科技有限公司;常州市第二人民醫(yī)院;School
【基金】：國家自然科學(xué)基金(批準(zhǔn)號:21374012)資助~~
【分類號】：Q813.1;R318.08

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