本文選題：納米羥基磷灰石 + MC3T3-E1　； 參考：《河北大學(xué)》2014年碩士論文

【摘要】：隨著納米材料的廣泛應(yīng)用，其潛在的生物安全性引起了科研工作者們廣泛關(guān)注。納米羥基磷灰石(HA)作為一種廣泛應(yīng)用于骨組織修復(fù)和藥物的運(yùn)載的生物材料，，因此其生物安全性值得人們關(guān)注。本文以缺陷發(fā)光的HA棒狀納米顆粒作為研究對(duì)象，在SEM、XRD、DLS等手段的表征基礎(chǔ)之上，以成骨細(xì)胞系MC3T3-E1為細(xì)胞模型，通過細(xì)胞存活率的檢測(cè)，納米顆粒的攝取和定位，細(xì)胞外乳酸脫氫酶活性的檢測(cè)，細(xì)胞內(nèi)活性氧表達(dá)水平和線粒體膜電位的檢測(cè)、及細(xì)胞凋亡檢測(cè)等手段在細(xì)胞水平上研究了納米HA對(duì)成骨細(xì)胞的生物學(xué)效應(yīng)。此外通過DNA凝膠電泳和Caspase-3基因的表達(dá)的檢測(cè)從分子水平上研究了其誘發(fā)細(xì)胞毒性的機(jī)制。結(jié)果表明：納米HA以大胞飲方式進(jìn)入細(xì)胞，主要集中在溶酶體中，少量在線粒體；進(jìn)入細(xì)胞內(nèi)的納米顆�？梢鸺�(xì)胞內(nèi)活性氧水平升高和線粒體膜電位降低，從而抑制細(xì)胞增殖。進(jìn)一步研究表明，納米HA可引起DNA損傷將細(xì)胞周期阻滯在S期，并通過上調(diào)Caspase-3基因引起細(xì)胞凋亡。
[Abstract]:With the wide application of nanomaterials, their potential biosafety has attracted wide attention of researchers. Nano-hydroxyapatite (HA) is widely used as a biomaterial for bone tissue repair and drug delivery. In this paper, the defect luminescent HA rod nanoparticles were used as the object of study. On the basis of the characterization of SEMX XRDLS, the osteoblast cell line MC3T3-E1 was used as the cell model. The survival rate of the cells and the uptake and localization of the nanoparticles were determined. The biological effects of nano-HA on osteoblasts were studied at the cellular level by detecting the activity of extracellular lactate dehydrogenase, the expression of reactive oxygen species and mitochondrial membrane potential, and the apoptosis of osteoblasts. In addition, the mechanism of cytotoxicity induced by DNA gel electrophoresis and Caspase-3 gene expression was studied at the molecular level. The results showed that the HA nanoparticles entered the cells in the form of large cellular drink, mainly concentrated in lysosomes and a few in mitochondria, and the nanoparticles entering the cells could increase the level of reactive oxygen species and decrease the membrane potential of mitochondria. Thus inhibiting cell proliferation. Further studies showed that nano-HA could induce DNA damage and arrest cell cycle in S phase and induce apoptosis by up-regulating Caspase-3 gene.
【學(xué)位授予單位】：河北大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R318.08

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