本文選題：仿生 + 聚乳酸-羥基乙酸��； 參考：《中國(guó)矯形外科雜志》2014年15期

【摘要】：[目的]通過(guò)仿生礦化的方法構(gòu)建聚乳酸-羥基乙酸共聚物(poly lactide-co-glycolide PLGA)人工骨,檢測(cè)仿生礦化對(duì)PLGA生物學(xué)性能的影響。[方法]將三維多孔PLGA材料在模擬體液中礦化2周,構(gòu)建仿生礦化人工骨,應(yīng)用掃描電鏡進(jìn)行礦化物形貌觀察,X射線能譜分析鈣磷比值,X射線衍射儀和傅立葉轉(zhuǎn)換紅外光譜儀行礦化物物相分析。獲取大鼠骨髓基質(zhì)干細(xì)胞(bone mesenchymal stem cells,BMSCs),經(jīng)傳代培養(yǎng)后作為種子細(xì)胞與仿生礦化人工骨于體外聯(lián)合培養(yǎng),同時(shí)以未礦化的PLGA材料和煅燒松質(zhì)骨為對(duì)比,應(yīng)用RT-PCR的方法測(cè)定各組材料上大鼠BMSCs成骨基因表達(dá)情況,通過(guò)比較成骨基因表達(dá)時(shí)間先后次序和量的變化,表明各組材料對(duì)BMSCs向成骨細(xì)胞分化的影響。[結(jié)果]礦化后的PLGA材料網(wǎng)孔壁有磷灰石礦化層形成,礦化物主要成分為羥基磷灰石,鈣磷比為1.53,類似于人骨無(wú)機(jī)質(zhì)。大鼠BMSCs在三種材料上培養(yǎng)7 d后,堿性磷酸酶(alkaline phosphatase,ALP)相對(duì)表達(dá)水平煅燒松質(zhì)骨組礦化組未礦化組(P0.05);骨涎蛋白(bonesialoprotein,BSP)和骨鈣素(osteocalcin,OCN)在各組均沒(méi)有表達(dá)。培養(yǎng)14 d后ALP相對(duì)表達(dá)水平煅燒松質(zhì)骨組和礦化組無(wú)顯著性差別(P0.05),均明顯高于未礦化組(P0.05);煅燒松質(zhì)骨組BSP相對(duì)表達(dá)水平明顯高于礦化組(P0.05);OCN在煅燒松質(zhì)骨組有少量表達(dá)。培養(yǎng)21 d后ALP相對(duì)表達(dá)水平未礦化組礦化組煅燒松質(zhì)骨組(P0.05);BSP和OCN在表達(dá)水平顯示礦化組和煅燒松質(zhì)骨組沒(méi)有明顯差別(P0.05),均明顯高于未礦化組(P0.05)。[結(jié)論]PLGA仿生礦化人工骨能夠顯著提高骨髓基質(zhì)干細(xì)胞成骨基因的表達(dá),促進(jìn)其向成骨細(xì)胞分化。
[Abstract]:[objective] to construct poly lactide-co-glycolide PLGA artificial bone by biomimetic mineralization, and to detect the effect of biomimetic mineralization on the biological properties of poly (lactic acid-glycolic acid) copolymers. [methods] Three-dimensional porous PLGA material was mineralized in simulated body fluid for 2 weeks to construct biomimetic mineralized artificial bone. Scanning electron microscopy (SEM) was used to observe the morphology of mineralized matter. X-ray diffraction and Fourier transform infrared spectroscopy (FTIR) were used to analyze the phase of mineralized matter. Rat bone marrow stromal stem cells (BMSCs) were obtained and cultured as seed cells and biomimetic mineralized artificial bone in vitro, and compared with unmineralized mesenchymal stem materials and calcined cancellous bone. The expression of osteogenic genes in BMSCs was determined by RT-PCR. The effects of each group on the differentiation of BMSCs into osteoblasts were compared by comparing the time sequence and quantity of expression of osteogenic genes. [results] there was apatite mineralized layer in the porous wall of PLGA material after mineralization. The main component of the mineralized material was hydroxyapatite and the ratio of calcium to phosphorus was 1.53, which was similar to the inorganic substance of human bone. Rat BMSCs cultured on three materials for 7 days, the relative expression level of alkaline phosphatase (ALP) in calcined cancellous bone mineralization group was P0.05a, bone sialoprotein (BSPP) and osteocalcin OCNs were not expressed in each group. The relative expression level of ALP in calcined cancellous bone group and mineralized group was significantly higher than that in unmineralized bone group (P 0.05), and the relative expression level of BSP in calcined cancellous bone group was significantly higher than that in mineralization group (P 0.05 OCN). After 21 days of culture, the relative expression level of ALP in calcined cancellous bone group (P0.05BSP) and OCN (OCN) group showed no significant difference between the mineralized group and calcined cancellous bone group, which was significantly higher than that in the unmineralized group (P0.05). [conclusion] PLGA biomimetic mineralized artificial bone can significantly increase the expression of osteogenic gene in bone marrow stromal cells and promote its differentiation into osteoblasts.
【作者單位】： 山東省泰安市中心醫(yī)院創(chuàng)傷骨科;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院骨科;
【分類號(hào)】：R318.08

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