本文選題：甲狀軟骨 + 軟骨細(xì)胞�。� 參考：《中國組織工程研究》2016年12期

【摘要】：背景:將聚羥基乙酸支架與軟骨細(xì)胞復(fù)合,可獲得組織工程人工骨。目的:觀察同種異體軟骨細(xì)胞-聚羥基乙酸支架復(fù)合物修復(fù)乳兔喉甲狀軟骨缺損的效果。方法:取新西蘭成年大白兔20只,構(gòu)建喉甲狀軟骨缺損模型后隨機(jī)分組,實(shí)驗(yàn)組于缺損處植入同種異體軟骨細(xì)胞-聚羥基乙酸支架復(fù)合物,對(duì)照組于缺損處植入聚羥基乙酸支架。植入后4,8周進(jìn)行大體觀察、組織學(xué)觀察。結(jié)果與結(jié)論:(1)大體觀察結(jié)果:植入后4周,實(shí)驗(yàn)組軟骨缺損得到一定的修復(fù),修復(fù)區(qū)域未出現(xiàn)壞死;對(duì)照組缺損區(qū)域填充有肌肉和結(jié)締組織。植入后8周,實(shí)驗(yàn)組軟骨缺損得到進(jìn)一步修復(fù),修復(fù)界限不明顯;對(duì)照組軟骨缺損未得到修復(fù),與周圍正常軟骨之間存在明顯界限。(2)免疫組織化學(xué)染色結(jié)果:實(shí)驗(yàn)組植入后4,8周的Ⅱ型膠原表達(dá)均高于對(duì)照組(P0.05)。結(jié)果表明同種異體軟骨細(xì)胞-聚羥基乙酸支架復(fù)合物可促進(jìn)乳兔喉甲狀軟骨缺損的修復(fù)。
[Abstract]:Background: tissue engineering artificial bone can be obtained by combining polyglycolic acid scaffold with chondrocytes. Aim: to observe the effect of allogeneic chondrocyte-polyglycolic acid scaffold complex in repairing laryngeal thyroid cartilage defect in milk rabbits. Methods: twenty New Zealand adult rabbits were randomly divided into two groups after the laryngeal thyroid cartilage defect model was established. The experimental group implanted allogenic chondrocyte-polyglycolic acid scaffold complex in the defect. In the control group, polyglycolic acid stent was implanted at the defect. Gross observation and histological observation were made 8 weeks after implantation. Results and conclusion: 4 weeks after implantation, the cartilage defects in the experimental group were repaired to a certain extent, and no necrosis was found in the repaired region, while the defect area in the control group was filled with muscle and connective tissue. 8 weeks after implantation, the cartilage defects in the experimental group were further repaired, the repair limits were not obvious, and the cartilage defects in the control group were not repaired. The results of immunohistochemical staining showed that the expression of type 鈪，

本文編號(hào)：2018826