本文選題：干細(xì)胞 + 股骨頭壞死　； 參考：《中國(guó)組織工程研究》2017年13期

【摘要】：背景:課題組前期研究已經(jīng)證實(shí)乙醇能促進(jìn)骨髓間充質(zhì)干細(xì)胞成脂分化,上調(diào)腫瘤壞死因子α成脂通路蛋白PPARγ,aP 2的表達(dá)。鋅轉(zhuǎn)運(yùn)蛋白1(Zrt/Irt-like Protein 1,ZIP1)作為ZIP蛋白家族的成員與骨代謝和成骨分化有密切的關(guān)系。目的:觀察ZIP1 siR NA轉(zhuǎn)染的骨髓間充質(zhì)干細(xì)胞對(duì)腫瘤壞死因子α信號(hào)成脂通路的影響。方法:分離培養(yǎng)兔骨髓間充質(zhì)干細(xì)胞,使用0.03,0.09,0.15,0.21 mol/L乙醇進(jìn)行培養(yǎng),再使用ZIP1 siR NA轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞,同時(shí)對(duì)兔骨髓間充質(zhì)干細(xì)胞進(jìn)行ZIP1轉(zhuǎn)染培養(yǎng)。結(jié)果與結(jié)論:(1)aP 2,PPARγ蛋白的表達(dá):不同濃度乙醇培養(yǎng)aP 2,PPARγ蛋白表達(dá)均較明顯升高(P0.05);除0.03 mol/L乙醇組之外,其他濃度乙醇組三酰甘油水平明顯升高(P0.05);沉默ZIP1基因轉(zhuǎn)染乙醇培養(yǎng)的骨髓間充質(zhì)干細(xì)胞后,各乙醇濃度組a P2,PPARγ蛋白的表達(dá)量及三酰甘油水平均明顯升高(P0.05),而骨髓間充質(zhì)干細(xì)胞經(jīng)ZIP1表達(dá)載體轉(zhuǎn)染后,a P2,PPARγ蛋白表達(dá)明顯降低(P0.05);(2)結(jié)果表明:沉默ZIP1表達(dá)能激活骨髓間充質(zhì)干細(xì)胞的腫瘤壞死因子α信號(hào)成脂通路的表達(dá),從而發(fā)揮促進(jìn)細(xì)胞的成脂分化能力。
[Abstract]:Background: Previous studies have confirmed that ethanol can promote lipid differentiation in bone marrow mesenchymal stem cells and up regulate the expression of tumor necrosis factor alpha lipoprotein pathway protein PPAR gamma, aP 2. Zinc transporter 1 (Zrt/Irt-like Protein 1, ZIP1) as a member of the ZIP protein family has a close relationship with bone Xie Hecheng bone differentiation. Objective: To observe ZIP1 siR The effect of NA transfected bone marrow mesenchymal stem cells on the lipid pathway of tumor necrosis factor alpha. Methods: the rabbit bone marrow mesenchymal stem cells were isolated and cultured, 0.03,0.09,0.15,0.21 mol/L ethanol was used to culture, and ZIP1 siR NA was used to transfect bone marrow mesenchymal stem cells, and the rabbit bone marrow mesenchymal stem cells were transfected with ZIP1 transfection and culture. Results and conclusions: (1) expression of aP 2, PPAR gamma protein: the expression of aP 2 and PPAR gamma in ethanol culture at different concentrations increased significantly (P0.05), and the level of three acyl glycerol in other ethanol groups except 0.03 mol/L ethanol group was significantly increased (P0.05), and the concentration of ethanol concentration a P2, PPAR gamma after transfection of ZIP1 gene transfected with ethyl alcohol in bone marrow mesenchymal stem cells. The expression of protein and the level of three glycerol increased significantly (P0.05), while the expression of a P2 and PPAR gamma in bone marrow mesenchymal stem cells was significantly reduced after transfection of ZIP1 expression vector (P0.05). (2) the results showed that silent ZIP1 expression could activate the expression of swell necrosis factor alpha signaling pathway in bone marrow mesenchymal stem cells, and thus play an important role in promoting the expression of lipid pathway. The adipocyte differentiation capacity of the cell.
【作者單位】： 廣西中醫(yī)藥大學(xué)附屬瑞康醫(yī)院骨科;廣西中醫(yī)藥大學(xué);
【基金】：廣西自然科學(xué)基金課題(2015GXNSFAA139136) 廣西高校青年教師基礎(chǔ)能力提升項(xiàng)目(KY2016YB204) 廣西衛(wèi)生廳重點(diǎn)課題(S201419-05) 廣西中醫(yī)藥大學(xué)校級(jí)科研項(xiàng)目(2015LX027) 2016年研究生教育創(chuàng)新計(jì)劃資助項(xiàng)目立項(xiàng)課題(YJS201650) 2014年廣西中醫(yī)藥大學(xué)校級(jí)培育學(xué)科-骨外科學(xué)建設(shè)項(xiàng)目[J13167(7)]~~
【分類號(hào)】：R318.08;R681.8
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本文編號(hào)：1993456