本文選題：砭石 + 生物相容性��； 參考：《中南大學(xué)》2013年碩士論文

【摘要】：目的： 通過動(dòng)物體內(nèi)植入、體外細(xì)胞毒性及動(dòng)物皮膚刺激實(shí)驗(yàn)評(píng)估砭石的生物相容性。 方法： 根據(jù)GB/T1688.和IS0/10993.標(biāo)準(zhǔn)規(guī)定的方法對(duì)泗濱砭石進(jìn)行動(dòng)物體內(nèi)植入、體外細(xì)胞毒性及動(dòng)物皮膚刺激實(shí)驗(yàn)。 1.動(dòng)物體內(nèi)植入實(shí)驗(yàn)：①實(shí)驗(yàn)材料選取泗濱砭石,硅膠T型支架。②分組：植入砭石為實(shí)驗(yàn)組,植入硅膠T型支架為對(duì)照組,大鼠于腰部切開后縫合,作為空白組。分別于手術(shù)后7、30、90天麻醉后處死大鼠,取出埋植模塊及周圍少量包裹組織。HE染色光鏡下觀察埋入材料周圍組織、細(xì)胞反應(yīng)和變化。 2.細(xì)胞毒性實(shí)驗(yàn)：培養(yǎng)小鼠成纖維細(xì)胞(L929),采用不同濃度的砭石浸提液、硅膠浸提液、1640培養(yǎng)基分別培養(yǎng)細(xì)胞,在第1、3、7天通過MTT法染色,對(duì)砭石的細(xì)胞毒性進(jìn)行評(píng)價(jià)。 3.動(dòng)物皮膚刺激實(shí)驗(yàn)：砭石研磨成粉狀為實(shí)驗(yàn)組,無菌脫敏醫(yī)用紗布為對(duì)照組,皮膚固定敷貼片至少4h。單次接觸試驗(yàn)時(shí),在除去敷貼片后1h,24h,48h、72h,觀察至14d,記錄各接觸部位情況并評(píng)分。 結(jié)果： 1.動(dòng)物體內(nèi)植入實(shí)驗(yàn)：植入后7天,兩組周圍組織均見毛細(xì)血管增多,擴(kuò)張充血,中性粒細(xì)胞、淋巴細(xì)胞增多浸潤圍繞毛細(xì)血管,材料周圍組織未發(fā)現(xiàn)囊壁形成。植入后30天周圍組織見中等量新生毛細(xì)血管、中等偏少炎性細(xì)胞(包括淋巴、中性粒細(xì)胞),見少量疏松的纖維囊壁結(jié)構(gòu)。植入后90天周圍組織見致密、質(zhì)薄、透明纖維囊壁結(jié)構(gòu),兩種材料之間差異無顯著性意義(P0.05)。 2.體外細(xì)胞毒性實(shí)驗(yàn)：不同濃度砭石浸提液對(duì)L929細(xì)胞均有促進(jìn)增殖作用(P0.01),在第1、3、7天各濃度之間均無差異性(P0.05),砭石的細(xì)胞毒性等級(jí)評(píng)分為0級(jí)-1級(jí)。 3.動(dòng)物皮膚刺激實(shí)驗(yàn)：兩組材料接觸5h后,除去敷貼片后1h、24h、48h和72h在直視及5倍放大鏡下觀察,皮膚無色素沉著、無紅斑、焦痂形成,無水腫。術(shù)后連續(xù)觀察14d,原發(fā)性刺激指數(shù)(P工I)0分,兩組無明顯差異。 結(jié)論： 砭石具有良好的生物相容性,為臨床應(yīng)用提供了一定的實(shí)驗(yàn)依據(jù)。
[Abstract]:Objective: Biocompatibility of Bian-stone was evaluated by in vivo implantation, in vitro cytotoxicity and skin stimulation. Methods: According to GB / T 1688. And IS0 / 10993. The standard method was used to implant Sibin stone in vivo and to test in vitro cytotoxicity and skin irritation. 1. Sibin stone and silica gel T stent were divided into two groups: the experimental group was implanted with silica gel T stent, the control group was implanted with silica gel T stent, and the rats were sutured after lumbar incision. The rats were anesthetized 7 days after operation for 30 days. The implanted modules and a small amount of surrounding tissue were taken out. The tissue around the implanted material was observed under light microscope and the cell reaction and changes were observed under light microscope. 2. Cytotoxicity test: mouse fibroblasts were cultured in different concentrations of Bian stone extract and silica gel extract on the medium of 1640. The cytotoxicity of Bian stone was evaluated by MTT staining on the 3rd day of the first day. 3. Animal skin irritation test: the stone was ground into powder as experimental group, sterile desensitized medical gauze as control group, and skin fixation application for at least 4 hours. During the single contact test, the contact sites were recorded and evaluated at 1 h, 24 h, 48 h and 72 h after the application was removed. Results: 1. In vivo implantation experiment: 7 days after implantation, capillary increased, dilated and hyperemia, neutrophil and lymphocyte infiltration were observed in the surrounding tissues of both groups, and no cyst wall was found in the surrounding tissues. 30 days after implantation, a moderate amount of neocapillaries was found in the surrounding tissues, and a few loose fibrous cystic wall structures were observed in the medium less inflammatory cells (including lymph nodes, neutrophils and neutrophilic granulocytes). The surrounding tissue was dense, thin and transparent fiber wall structure was found 90 days after implantation. There was no significant difference between the two materials (P 0.05). 2. In vitro cytotoxicity test: different concentrations of Bian stone extract could promote the proliferation of L929 cells. There was no difference between different concentrations on the 1st day 3 ~ (th) day. The cytotoxicity grade of Bian stone was grade 0 ~ (-1). 3. Animal skin stimulation test: after 5 hours of contact with the two groups of materials, the skin was observed under direct vision and 5 times magnifying glass at 24 h, 48 h and 72 h after application. There was no pigmentation, no erythema, no scab formation and no edema. After 14 days, there was no significant difference between the two groups. Conclusion: Bian stone has good biocompatibility and provides some experimental basis for clinical application.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R318.08

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本文編號(hào)：1988875