發(fā)布時(shí)間：2018-06-01 09:31

  本文選題：細(xì)胞電穿孔 + 細(xì)胞電融合��； 參考：《揚(yáng)州大學(xué)》2012年碩士論文

【摘要】：細(xì)胞融合技術(shù)廣泛的應(yīng)用于遺傳學(xué)、細(xì)胞學(xué)、病毒學(xué)、免疫學(xué)等多種學(xué)科的研究工作之中,并日益成為生物醫(yī)藥研發(fā)工作中的一種重要技術(shù)手段。在目前的多種融合技術(shù)中,電穿孔融合技術(shù)是最具有市場(chǎng)應(yīng)用前景的一種。 本論文根據(jù)以往細(xì)胞電融合實(shí)驗(yàn)的經(jīng)驗(yàn),和生物物理學(xué)理論上的支持,結(jié)合最新電子技術(shù)的發(fā)展,制定了儀器整體的設(shè)計(jì)方案。在儀器的設(shè)計(jì)中,通過(guò)對(duì)以單片機(jī)為核心與以可編程邏輯器件(FPGA)為核心的脈沖控制單元進(jìn)行設(shè)計(jì)、測(cè)試和比較,確立了以FPGA為核心的脈沖控制單元的設(shè)計(jì)方案,其中對(duì)FPGA的設(shè)計(jì)過(guò)程中加入了以Nios Ⅱ軟核為核心的SOPC系統(tǒng)的設(shè)計(jì)。同時(shí)對(duì)以光電耦合器和鐵氧體變壓器為主的電氣隔離設(shè)備與POWER MOSFET的驅(qū)動(dòng)設(shè)備進(jìn)行設(shè)計(jì)、測(cè)試和比較,確立了以光電耦合器為電氣隔離設(shè)備的POWER MOSFET的驅(qū)動(dòng)設(shè)計(jì)為儀器的脈沖信號(hào)傳輸和放大的電路。由于儀器涉及到高壓部分,為了儀器操作者的安全,在儀器中加入了安全模塊的設(shè)計(jì)。同時(shí)為了儀器的正常運(yùn)轉(zhuǎn),還增加了電源模塊的設(shè)計(jì)。 最后經(jīng)過(guò)測(cè)試和驗(yàn)證,儀器可實(shí)現(xiàn)脈寬在1到300μS的脈沖精確輸出,誤差范圍控制在0.15μS范圍,并可實(shí)現(xiàn)312V高壓脈沖的輸出,滿足儀器最初的設(shè)計(jì)要求,并符合生物物理理論上的要求。
[Abstract]:Cell fusion technology is widely used in genetics, cytology, virology, immunology and other disciplines, and has increasingly become an important technical means in the research and development of biomedicine. Electroporation fusion is one of the most promising fusion technologies in the market. Based on the experience of previous cell electrofusion experiments and the theoretical support of biophysics, combined with the development of the latest electronic technology, the whole design scheme of the instrument is developed in this paper. In the design of the instrument, by designing, testing and comparing the pulse control unit with MCU as the core and programmable logic device (FPGA) as the core, the design scheme of the pulse control unit with FPGA as the core is established. In the process of FPGA design, the design of SOPC system based on Nios 鈪，

本文編號(hào)：1963706