本文選題：殼聚糖 + 明膠　； 參考：《中國組織工程研究》2015年30期

【摘要】：背景:課題組前期研制了新型明膠-殼聚糖-羥基磷灰石-米諾環(huán)素仿生納米復(fù)合材料。目的:觀察明膠-殼聚糖-羥基磷灰石-米諾環(huán)素仿生納米復(fù)合材料修復(fù)兔橈骨缺損的效果。方法:取30只成年健康新西蘭大白兔,在一側(cè)橈骨上端制作約15 mm×6 mm的臨界性腔隙性骨缺損,隨機(jī)分為實(shí)驗(yàn)組(n=15)、自體骨移植組(n=10)和空白對(duì)照組(n=5),實(shí)驗(yàn)組將明膠-殼聚糖-羥基磷灰石-米諾環(huán)素仿生納米復(fù)合材料植入骨缺損處,自體骨移植組從對(duì)側(cè)橈骨取等長橈骨植入骨缺損處,空白對(duì)照組不做任何處理。于術(shù)后2,4,8,12周分別行大體觀察、組織學(xué)觀察及X射線平片檢查。結(jié)果與結(jié)論:植入后12周,實(shí)驗(yàn)組骨缺損區(qū)有明顯血管生成,骨性愈合完全,植入復(fù)合材料消失,出現(xiàn)板層骨結(jié)構(gòu),可見小血管,新生骨邊緣與原骨邊緣連接,表現(xiàn)成骨的連續(xù)性,密度較原骨稍降低,骨缺損區(qū)不明顯;自體骨移植組骨性愈合,骨小梁重建較明顯,板層骨較成熟,髓腔再通,骨折線完全消失,骨密度與原骨完全一致;空白對(duì)照組髓腔無明顯成骨現(xiàn)象,大量纖維組織及炎癥細(xì)胞浸潤,骨折斷端未愈合。表明明膠-殼聚糖-羥基磷灰石-米諾環(huán)素仿生納米復(fù)合材料可明顯促進(jìn)臨界性骨缺損修復(fù),效果與自體骨移植相當(dāng)。
[Abstract]:Background: a novel gelatin-chitosan-hydroxyapatite-minocycline biomimetic nanocomposite was developed. Aim: to observe the effect of gelatin-chitosan-hydroxyapatite-minocycline biomimetic nanocomposite in repairing rabbit radius defect. Methods: a critical lacunar bone defect of 15 mm 脳 6 mm was made in 30 healthy adult New Zealand rabbits at the upper end of the radius. The experimental group was randomly divided into two groups: the experimental group (n = 15), the autogenous bone graft group (n = 10) and the blank control group (n = 5). The experimental group implanted bone defect with gelatin, chitosan, hydroxyapatite and minocycline biomimetic nanocomposites. In the autograft group, the isometric radius was taken from the contralateral radius and implanted into the defect, while the blank control group was not treated. Gross observation, histological observation and X-ray plain-film examination were performed 12 weeks after operation. Results and conclusion: at 12 weeks after implantation, there was obvious angiogenesis in the bone defect area in the experimental group, the bone union was complete, the composite material disappeared, the lamellar bone structure appeared, small vessels could be seen, and the new bone margin was connected with the original bone edge. In autogenous bone transplantation group, bone healing, trabecular bone reconstruction, lamellar bone maturation, medullary cavity recanalization, fracture line disappeared completely, bone density was completely consistent with original bone. In the blank control group, there was no obvious osteogenesis, a large number of fibrous tissue and inflammatory cells infiltrated, and the broken end of fracture was not healed. The results showed that the biomimetic nanocomposites of gelatin chitosan hydroxyapatite and minocycline could promote the repair of critical bone defect and the effect was similar to that of autogenous bone graft.
【作者單位】： 安徽醫(yī)科大學(xué)附屬第一醫(yī)院骨科;安徽醫(yī)科大學(xué)附屬口腔醫(yī)院;安徽口腔疾病研究省級(jí)實(shí)驗(yàn)室;
【分類號(hào)】：R318.08

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本文編號(hào)：1956705