本文選題：Nano-HA + 電化學(xué)沉積技術(shù)��； 參考：《浙江大學(xué)》2012年碩士論文

【摘要】：目的： 納米級(jí)羥磷友石(Nano-hydroxyapatite, Nano-HA)與人體內(nèi)羥磷灰石具有相似性,具有較好的生物學(xué)性能。電化學(xué)沉積技術(shù)可在多孔純鈦表面制備Nano-HA,且不破壞純鈦表面的多孔結(jié)構(gòu)。本研究利用電化學(xué)沉積技術(shù)在多孔純鈦表面制備Nano-HA涂層,研究Nano-HA涂層對(duì)成骨細(xì)胞生物學(xué)活性的影響以及骨質(zhì)疏松癥種植體骨結(jié)合的影響。 材料與方法： 純鈦鈦片和種植體經(jīng)大顆粒金鋼砂噴砂,HF/HNO3和HCl/H2SO4酸蝕。Nano-HA涂層制備采用兩電極系統(tǒng),以粗化鈦片和種植體為陰極,鉑片為陽極,400ml燒杯為電解槽,在含Ca(NO3)2(0.6mM)和NH4H2PO4(0.36mM)的200ml電解液中,在一定的溫度和時(shí)間下,恒電壓電沉積。Nano-HA涂層鈦片和種植體作為實(shí)驗(yàn)組,多孔表面鈦片和種植體作為對(duì)照組。場發(fā)射掃描電鏡(FSEM)、X射線衍射儀(XRD)、紅外光譜儀(FTIR)分析涂層表面結(jié)構(gòu),表面粗糙度測試儀(TR240)檢測表面粗糙度。實(shí)驗(yàn)一：將小鼠前成骨細(xì)胞(MC3T3-E1)接種于上述兩組鈦片上,測定細(xì)胞總蛋白、堿性磷酸酶(Alkaline phosphatase, ALP)、骨鈣素(Osteocalcin).實(shí)驗(yàn)二：建立大鼠骨質(zhì)疏松癥動(dòng)物模型,將對(duì)照組和實(shí)驗(yàn)組種植體植入大鼠兩側(cè)脛骨遠(yuǎn)端,術(shù)后4周和12周處死動(dòng)物,制作硬組織切片,采用亞甲基藍(lán)-堿性品紅法染色,對(duì)種植體-骨接觸率和種植體螺紋內(nèi)骨密度進(jìn)行測量；采用便攜式數(shù)字扭力測試儀(BS30)觀察分析種植體和骨組織的結(jié)合力,并對(duì)兩組種植體的扭力值進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果： 鈦片表面經(jīng)噴砂-雙重酸蝕處理后形成多級(jí)微孔、波浪狀的形貌；電化學(xué)沉積技術(shù)在鈦片表面制備截面為規(guī)則六邊形的納米級(jí)晶體。XRD和FTIR分析表明該涂層是標(biāo)準(zhǔn)的HA。對(duì)照組和HA涂層組的表面粗糙度(Ra)平均值分別為1.185±0.061和1.167±0.096。細(xì)胞實(shí)驗(yàn)的結(jié)果顯示HA組能促進(jìn)MC3T3-E1細(xì)胞增殖,同時(shí)也能增強(qiáng)ALP活性和OC分泌。動(dòng)物實(shí)驗(yàn)統(tǒng)計(jì)學(xué)表明,術(shù)后4周和12周Nano-HA涂層種植體明顯增加骨質(zhì)疏松癥大鼠種植體與新骨接觸率和種植體螺紋內(nèi)骨密度(P0.05),增加種植體與新骨之間的結(jié)合力(P0.05)。 結(jié)論： 本實(shí)驗(yàn)證實(shí)該電化學(xué)沉積技術(shù)制備的Nano-HA涂層可促進(jìn)成骨細(xì)胞的生物學(xué)活性以及骨質(zhì)疏松癥種植體骨結(jié)合。
[Abstract]:Objective: Nano-hydroxyapatite (Nano-HA) is similar to human hydroxyapatite and has good biological properties. Nano-HA can be prepared on porous titanium surface by electrochemical deposition without destroying the porous structure of pure titanium surface. In this study, Nano-HA coatings were prepared on porous pure titanium by electrochemical deposition technique. The effects of Nano-HA coating on the biological activity of osteoblasts and the osseous bonding of osteoporosis implants were studied. Materials and methods: Pure titanium sheets and implants were coated with large grain au / HNO _ 3 and HCl/H2SO4 acid etching. Nano-HA was prepared by using a two-electrode system, in which roughened titanium plates and implants were used as cathode, platinum plates as anode and 400ml beaker as electrolyzer, in 200ml electrolyte containing Cagno _ 3N _ 3N _ 2O _ 2O _ 20.6mm) and NH _ 4H _ 2PO _ 4O _ 40.36mM). Nano-HA coated titanium plates and implants were used as experimental group and porous surface titanium plates and implants as control group at certain temperature and time. The surface structure of the coating was analyzed by FTIR, and the surface roughness was measured by the surface roughness tester TR240). Experiment 1: the mouse preosteoblast (MC3T3-E1) was inoculated on the above two groups of titanium tablets, and the total cell protein, alkaline phosphatase (ALP), osteocalcinine (Osteocalcinia) were determined. Experiment 2: the animal model of osteoporosis in rats was established. The implants of the control group and the experimental group were implanted into the distal tibia of the rats on both sides. The animals were killed 4 and 12 weeks after operation, and the hard tissue sections were made and stained by methylene blue alkaline fuchsin method. The implant-bone contact rate and bone mineral density in implant thread were measured, and the adhesive force of implant and bone tissue was observed and analyzed by means of portable digital torsion tester (BS30), and the torque values of two groups of implants were analyzed statistically. Results: After sandblasting and double acid etching, multistage micropores and wavy morphology were formed on the surface of titanium sheet, and nanocrystalline crystals with regular hexagonal cross section were prepared by electrochemical deposition technique. XRD and FTIR analysis showed that the coating was a standard HAA coating. The average values of surface roughness in the control group and HA coated group were 1.185 鹵0.061 and 1.167 鹵0.096, respectively. The results of cell experiment showed that HA could promote the proliferation of MC3T3-E1 cells, but also enhance the activity of ALP and the secretion of OC. Animal statistics showed that Nano-HA coated implants significantly increased the contact rate between implant and new bone and bone mineral density (P0.05) in implant thread, and increased the binding force between implant and new bone at 4 and 12 weeks postoperatively in rats with osteoporosis. Conclusion: The results showed that the Nano-HA coating prepared by the electrochemical deposition technique could promote the biological activity of osteoblasts and the osseointegration of osteoporosis implants.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊建華,張通和;強(qiáng)束流鉭、碳離子雙注入H13鋼的研究[J];中國表面工程;2004年02期

2 楊名宇;陳敏;李長敏;張慶瑜;;磁控濺射羥基磷灰石薄膜的研究現(xiàn)狀與展望[J];材料導(dǎo)報(bào);2006年04期

3 寇生中;程艷玲;郭燕;;磁控濺射法在生物材料表面制備羥基磷灰石涂層的研究現(xiàn)狀[J];材料導(dǎo)報(bào);2006年06期

4 孫彩云;何慶兵;李立;張隆平;吳護(hù)林;;磁控濺射法制備TiB_2涂層的研究進(jìn)展[J];材料導(dǎo)報(bào);2009年S1期

5 唐娟,崔振鐸,朱勝利,桑曉明,楊賢金;鈦及鈦合金仿生表面改性研究進(jìn)展[J];功能材料;2005年01期

6 劉慧穎;王學(xué)金;伊哲;王浪平;王小峰;艾紅軍;;氟離子注入鈦表面改性對(duì)成骨細(xì)胞黏著斑形成的影響[J];華西口腔醫(yī)學(xué)雜志;2008年02期

7 姜耕;汪炳華;;羥基磷灰石人工骨植體的制備及生物力學(xué)研究[J];長江大學(xué)學(xué)報(bào)(自科版)醫(yī)學(xué)卷;2008年03期

8 李亞男,劉洪臣,倪紫硯,王東勝,邱華;骨質(zhì)疏松狀態(tài)下牙種植體骨整合改變的實(shí)驗(yàn)研究[J];口腔頜面修復(fù)學(xué)雜志;2000年01期

9 王磊;陳建治;石玉龍;閆鳳英;孫桂蘭;;純鈦表面微弧氧化膜的結(jié)構(gòu)與成分分析[J];口腔頜面修復(fù)學(xué)雜志;2006年02期

10 龔沛;王欣宇;郭潔;;仿生法制備純鎂/羥基磷灰石復(fù)合涂層的研究[J];生物骨科材料與臨床研究;2008年04期

，

本文編號(hào)：1952012