本文選題：靜電紡絲 + 聚甲基丙烯酸甲酯��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2015年04期

【摘要】：目的探討具備有序或無(wú)序拓?fù)浣Y(jié)構(gòu)的聚甲基丙烯酸甲酯(polymethylmethacrylate,PMMA)電紡納米纖維材料作為原代大鼠背根神經(jīng)元(dorsal root ganglion neurons,DRGn)負(fù)載支架的可行性。方法構(gòu)建具有隨機(jī)或有序拓?fù)浣Y(jié)構(gòu)的PMMA電紡納米纖維;利用PMMA薄膜組作為對(duì)照,分離純化大鼠原代DRGn,利用慢病毒技術(shù)轉(zhuǎn)染綠色熒光蛋白基因作為顯色手段,分析在隨機(jī)及有序纖維支架上DRGn神經(jīng)突的生長(zhǎng)能力以及神經(jīng)突和電紡纖維的依存關(guān)系。結(jié)果大鼠原代DRGn能夠順利在PMMA材料上貼壁并生長(zhǎng),在培養(yǎng)第2天,較之平面環(huán)境,DRGn未見平均神經(jīng)突數(shù)量及神經(jīng)突長(zhǎng)度的明顯區(qū)別,電紡纖維的拓?fù)浣Y(jié)構(gòu)對(duì)于DRGn神經(jīng)突的生長(zhǎng)具有明顯的接觸引導(dǎo)作用,在有序電紡纖維上DRGn能夠生成和基質(zhì)纖維延伸方向一致的神經(jīng)突;通過(guò)GFP表達(dá)與背景纖維的合成圖,發(fā)現(xiàn)在隨機(jī)或有序電紡纖維上,DRGn神經(jīng)突均能循纖維向前生長(zhǎng),但相比隨機(jī)纖維,神經(jīng)突似乎更傾向于接受有序電紡纖維的引導(dǎo)。結(jié)論有序PMMA電紡納米纖維具有作為神經(jīng)損傷后大鼠DRGn負(fù)載支架的潛力。
[Abstract]:Objective to investigate the feasibility of polymethylmethacrylate PMMA (PMMA) electrospun nanofiber material with ordered or disordered topological structure as the primary dorsal root neuron dorsal root ganglion neurons DRGN loading scaffold. Methods PMMA electrospun nanofibers with random or ordered topological structure were constructed, the primary rat DRGN was isolated and purified by using PMMA film group as control, and the green fluorescent protein gene was transfected by lentivirus technique as a means of color rendering. The growth ability of DRGn nerve process and the dependence of nerve process and electrospun fiber on random and ordered fiber scaffolds were analyzed. Results the primary rat DRGn was able to adhere to the wall and grow smoothly on the PMMA material. On the second day of culture, there was no significant difference in the average number of neurites and the length of the neurite compared with the plane environment. The topological structure of the electrospun fiber has obvious contact guiding effect on the growth of the DRGn neurite. On the ordered electrospun fiber, DRGn can produce the neurite which is consistent with the extension direction of the matrix fiber, and is expressed with the synthesis diagram of the background fiber through the GFP expression. It was found that DRGn neurites could grow forward on random or ordered electrospun fibers, but they seemed to be more inclined to accept the guidance of ordered electrospun fibers than random fibers. Conclusion ordered PMMA electrospun nanofibers have the potential as DRGn loaded scaffolds after nerve injury in rats.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院神經(jīng)外科;重慶中山醫(yī)院藥劑科;
【基金】：國(guó)家臨床重點(diǎn)�？平ㄔO(shè)項(xiàng)目(財(cái)社[2011]170號(hào)) 國(guó)家自然科學(xué)基金(81100906) 重慶市科委前沿與應(yīng)用基礎(chǔ)研究計(jì)劃(CSTC2014jcyj A10061)~~
【分類號(hào)】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 夏海堅(jiān);劉丹;鐘東;晏怡;夏永智;唐文淵;孫曉川;;聚甲基丙烯酸甲酯靜電紡絲納米纖維的拓?fù)浣Y(jié)構(gòu)對(duì)于PC12細(xì)胞神經(jīng)突生長(zhǎng)能力和方向的影響[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2014年04期

2 夏海堅(jiān);劉丹;鐘東;晏怡;夏永智;唐文淵;孫曉川;;具備不同拓?fù)浣Y(jié)構(gòu)的聚甲基丙烯酸甲酯靜電紡絲納米纖維細(xì)胞支架的構(gòu)建及鑒定[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2014年04期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 夏海堅(jiān);劉丹;鐘東;晏怡;夏永智;唐文淵;孫曉川;;聚甲基丙烯酸甲酯靜電紡絲納米纖維的拓?fù)浣Y(jié)構(gòu)對(duì)于PC12細(xì)胞神經(jīng)突生長(zhǎng)能力和方向的影響[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2014年04期

2 夏海堅(jiān);劉丹;鐘東;夏永智;晏怡;唐文淵;孫曉川;;聚甲基丙烯酸甲酯靜電紡絲納米纖維對(duì)大鼠原代星形膠質(zhì)細(xì)胞生長(zhǎng)的影響[J];南方醫(yī)科大學(xué)學(xué)報(bào);2014年11期

3 付丹丹;;促骨髓基質(zhì)干細(xì)胞成骨分化因子研究現(xiàn)狀[J];菏澤醫(yī)學(xué)�？茖W(xué)校學(xué)報(bào);2014年02期

4 戴l，

本文編號(hào)：1919008