本文選題：純鎂 + 微弧氧化�。� 參考：《佳木斯大學(xué)》2015年碩士論文

【摘要】：目的:將純鎂超聲微弧氧化后進(jìn)行化學(xué)鍍鋅,對涂層進(jìn)行成骨細(xì)胞生物相容性檢測,評價純鎂微弧氧化經(jīng)化學(xué)鍍鋅的生物涂層的生物相容性。方法:將純鎂制備成φ10mm×1.5mm的圓形鎂片,超聲微弧氧化后,進(jìn)行化學(xué)鍍鋅,制備出表面含鋅的微弧氧化純鎂涂層材料。實(shí)驗(yàn)分為三組:A組為超聲微弧氧化組(MAO),B組為化學(xué)鍍鋅10min的MAO純鎂(MAO-Zn),C組為堿處理后化學(xué)鍍鋅10min的MAO純鎂(MAO-NaOH-Zn),(A組為對照組,B組及C組為實(shí)驗(yàn)組)用掃描電鏡(SEM)和能譜分析(EDS)分別測量與研究各組試件表面的形態(tài)與成分,激光共聚焦顯微鏡觀察成骨細(xì)胞的早期形態(tài),用cck8法檢測細(xì)胞增殖,并檢測成骨細(xì)胞ALP活性。結(jié)果:掃描電鏡觀察經(jīng)超聲微弧氧化處理的試件表面粗糙,多孔,起伏不平,涂層的主要元素有Mg、O、Si、Zn。掃描電鏡和激光共聚焦顯示C組和B組表面細(xì)胞的生長和黏附均優(yōu)于A組,C組優(yōu)于B組。CCK-8和ALP的檢測結(jié)果顯示三組材料表面細(xì)胞的增殖、活性為C組B組A組,三組的比較差異均具有統(tǒng)計學(xué)意義。結(jié)論:含鋅MAO涂層的鎂具有良好的生物相容性,而經(jīng)堿處理后的鍍鋅涂層生物相容性最佳。
[Abstract]:Aim: to evaluate the biocompatibility of electroless zinc plating coating on pure magnesium by ultrasonic microarc oxidation. Methods: pure magnesium was prepared into 蠁 10mm 脳 1.5mm circular magnesium sheet. After ultrasonic micro-arc oxidation, electroless zinc plating was carried out to prepare pure magnesium coating material containing zinc on the surface. The experiment was divided into three groups: group A: ultrasonic micro-arc oxidation group, group B, electroless galvanized 10min, MAO pure magnesium, MAO-ZnOZN, group C, MAO, pure magnesium, MAO, pure magnesium, 10min, after alkali treatment, control group, group B, and group C, as control group, group B and group C as experimental groups) and energy spectrum scores. The surface morphology and composition of each group of specimens were measured and studied by EDS. The early morphology of osteoblasts was observed by laser confocal microscopy. The proliferation and ALP activity of osteoblasts were detected by cck8 assay. Results: the surface of the samples treated by ultrasonic micro-arc oxidation was rough, porous and undulating, and the main elements of the coating were MgO / SiZn. The results of scanning electron microscopy and laser confocal scanning showed that the growth and adhesion of surface cells in group C and group B were superior to those in group A. CCK-8 and ALP showed that the proliferation of surface cells in group C was better than that in group B, and the activity of cells in group C was group A. The differences among the three groups were statistically significant. Conclusion: the magnesium coating containing zinc has good biocompatibility, but the zinc coating after alkali treatment has the best biocompatibility.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 薛娟琴;吳川眉;;超聲波對溶液性質(zhì)的影響[J];金屬世界;2008年01期

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