本文選題：去細(xì)胞 + 胰腺　； 參考：《解剖學(xué)報(bào)》2014年04期

【摘要】：目的通過(guò)離體灌注化學(xué)去垢劑的方法制備大鼠胰腺去細(xì)胞天然生物支架,并對(duì)支架的完整性、生物相容性進(jìn)行檢驗(yàn)。方法健康成年SD大鼠30只,分別經(jīng)膽管與血管兩條途徑灌注十二烷基硫酸鈉和曲亞通X-100等藥品洗脫,獲取胰腺去細(xì)胞生物支架。通過(guò)HE染色、掃描電子顯微鏡和透射電子顯微鏡等觀察細(xì)胞殘留于去細(xì)胞支架的生物學(xué)特性,分光光度計(jì)法鑒定殘留去垢劑量,酶聯(lián)免疫吸附劑測(cè)定法測(cè)定殘留支架蛋白含量及生長(zhǎng)因子,并用腹壁與皮下包埋實(shí)驗(yàn)檢驗(yàn)其炎癥反應(yīng),MTT法測(cè)定支架細(xì)胞毒性,內(nèi)皮細(xì)胞共培養(yǎng)測(cè)定支架生物相容性。結(jié)果 HE染色、掃描電子顯微鏡和透射電子顯微鏡觀察均表明去細(xì)胞支架無(wú)細(xì)胞殘留,細(xì)胞外支架連續(xù)性完好,脈管支架保存完整;藥物殘留小于規(guī)定標(biāo)準(zhǔn);細(xì)胞外支架生長(zhǎng)因子及支架蛋白存留量等指標(biāo)上血管組均優(yōu)于膽管組;腹壁與皮下包埋實(shí)驗(yàn)顯示,去細(xì)胞支架炎性反應(yīng)較對(duì)照組明顯減輕;MTT法顯示無(wú)細(xì)胞毒性;內(nèi)皮細(xì)胞共培養(yǎng)有黏附趨勢(shì)。結(jié)論使用兩種灌注法制備胰腺去細(xì)胞生物支架,均可將細(xì)胞去除徹底,支架生物相容性好。但就血管組細(xì)胞外支架保留完整性,生長(zhǎng)因子保留更多,則血管灌注途徑優(yōu)于膽管灌注途徑。
[Abstract]:Aim to prepare the acellular natural scaffold of rat pancreas by in vitro infusion of chemical defouling agent, and to test the integrity and biocompatibility of the scaffold. Methods 30 healthy adult SD rats were perfused with drugs such as sodium dodecyl sulfate and triazone X-100 respectively through bile duct and blood vessel to obtain acellular pancreatic scaffold. He staining, scanning electron microscope and transmission electron microscope were used to observe the biological characteristics of cells remaining in the scaffold, and the residual defouling dose was identified by spectrophotometer. The contents of residual stents protein and growth factors were determined by Elisa. The cytotoxicity of stents was determined by MTT assay and the biocompatibility of stents was determined by co-culture of endothelial cells. Results HE staining, scanning electron microscopy and transmission electron microscopy showed that there were no cell residues in the acellular stents, the extracellular stents were intact, the vascular stents were intact, and the drug residues were less than the prescribed standard. The upper vascular group was superior to the bile duct group in the indexes of extracellular stent growth factor and stenting protein retention, and the inflammatory response of acellular stents in abdominal wall and subcutaneous embedding was significantly less than that in the control group, and no cytotoxicity was observed by MTT assay. The co-culture of endothelial cells has the tendency of adhesion. Conclusion the acellular scaffolds of pancreas prepared by two kinds of perfusion methods can remove the cells completely and have good biocompatibility. However, the vascular perfusion pathway is superior to the bile duct perfusion pathway for the integrity of extracellular stent and more growth factors in vascular group.
【作者單位】： 溫州醫(yī)科大學(xué)第一臨床醫(yī)學(xué)院;溫州醫(yī)科大學(xué)生物支架移植與免疫研究所;溫州醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)中心;
【基金】：國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃資助項(xiàng)目(201310343006,201210343008) 浙江省自然科學(xué)基金資助項(xiàng)目(LY12H15002) 浙江省大學(xué)生科技創(chuàng)新活動(dòng)計(jì)劃“新苗人才計(jì)劃”資助項(xiàng)目(2012R413004) 浙江省教育廳資助項(xiàng)目(Y201327980)
【分類號(hào)】：R318.08

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