本文選題：硅橡膠 + 聚乙二醇甲醚丙烯酸酯　； 參考：《天津大學(xué)》2012年碩士論文

【摘要】：植入青光眼導(dǎo)流裝置已成為目前治療重癥青光眼病的主要方法,青光眼導(dǎo)流裝置主要采用的是硅橡膠材料,這種材料表面疏水性極強(qiáng),植入后容易在材料周圍形成很厚的纖維包囊,從而使導(dǎo)流裝置的功能喪失。本論文用聚乙二醇甲醚丙烯酸酯(PEGMEA)對(duì)醫(yī)用硅橡膠進(jìn)行了親水化表面改性,并對(duì)其改性原理、表面親疏水性、抗蛋白粘附性、抗細(xì)胞黏附性以及組織生物相容性進(jìn)行了系統(tǒng)評(píng)價(jià)。 在三氟甲磺酸的催化作用下,聚(甲基氫硅氧烷)中的部分含Si H基團(tuán)鏈段通過鏈交換反應(yīng)引入到硅橡膠表面,形成表面功能化硅橡膠。在鉑金催化劑的作用下,PEGMEA上的雙鍵與Si H基團(tuán)發(fā)生硅氫加成反應(yīng),從而使親水性聚乙二醇分子鏈接枝到硅橡膠表面。傅立葉變換衰減全反射紅外光譜(ATR-FTIR)和X-射線光電子能譜(XPS)結(jié)果顯示,通過化學(xué)接枝改性可將親水性PEGMEA分子鏈引入到硅橡膠表面;水接觸角測(cè)試結(jié)果證實(shí)接枝改性后硅橡膠表面的強(qiáng)疏水性得到一定程度的緩解;牛血清白蛋白粘附結(jié)果證實(shí),經(jīng)表面改性后的硅橡膠表面蛋白粘附量降低了約55%;而L929成纖維細(xì)胞黏附結(jié)果表明,PEGMEA改性硅橡膠上黏附的細(xì)胞數(shù)量只有原始硅橡膠的65%左右,且細(xì)胞基本呈圓球狀;細(xì)胞毒性評(píng)價(jià)結(jié)果顯示,改性后的硅橡膠細(xì)胞毒性等級(jí)為0級(jí)。 通過兔眼部組織植入法進(jìn)一步評(píng)價(jià)了親水化改性硅橡膠的體內(nèi)組織學(xué)反應(yīng),通過HE染色切片法對(duì)比研究了改性前后硅橡膠植入體的纖維包囊厚度、免疫細(xì)胞反應(yīng)等情況。結(jié)果表明,植入2周、4周和6周后,改性硅橡膠周圍產(chǎn)生的纖維包囊的厚度均小于未改性的醫(yī)用硅橡膠,綜合三個(gè)時(shí)間點(diǎn)的纖維包囊厚度統(tǒng)計(jì)數(shù)據(jù),改性后硅橡膠周圍產(chǎn)生的纖維包囊厚度比改性前平均減少約35%左右;但巨噬細(xì)胞數(shù)量統(tǒng)計(jì)結(jié)果顯示,與原始硅橡膠相比,表面改性硅橡膠并沒有明顯減少炎癥反應(yīng)。
[Abstract]:Implantation of glaucoma diversion device has become the main method for the treatment of severe glaucoma disease. The glaucoma diversion device mainly uses silicone rubber material, which has strong hydrophobicity on the surface. It is easy to form a thick fiber capsule around the material after implantation, which results in the loss of the function of the diversion device. In this paper, the hydrophilic surface modification of medical silicone rubber was carried out by polyethylene glycol methyl ether acrylate (PEGMEA), and its modification principle, surface hydrophobicity, anti-protein adhesion, anti-cell adhesion and tissue biocompatibility were systematically evaluated. In the presence of trifluoromethyl sulfonic acid (TFOS-catalyzed), some of the Si-containing H groups in the poly (methylhydrosiloxane) were introduced to the surface of silicone rubber by chain exchange reaction to form surface functionalized silicone rubber. Under the action of platinum catalyst, the double bonds on PEGMEA reacted with the Si-H group, thus the hydrophilic polyethylene glycol molecule was linked to the surface of silicone rubber. The results of Fourier transform attenuated total reflectance infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) show that the hydrophilic PEGMEA molecular chain can be introduced to the surface of silicone rubber by chemical grafting modification. The results of water contact angle test confirmed that the surface hydrophobicity of the modified silicone rubber was alleviated to a certain extent, and the adhesion of bovine serum albumin (BSA) was confirmed. The adhesion amount of surface protein of modified silicone rubber was reduced by about 55%, while the adhesion of L929 fibroblasts showed that the number of cells adhered to the modified silicone rubber was only about 65% of that of the original silicone rubber, and the cells were basically spherical. The results of cytotoxicity evaluation showed that the grade of cytotoxicity of modified silicone rubber was 0. The in vivo histological reaction of hydrophilic modified silicone rubber was further evaluated by rabbit eye tissue implantation method. The thickness of fiber capsule and immune cell reaction of silicone rubber implants before and after modification were compared by HE staining and section method. The results showed that the thickness of fiber cyst around modified silicone rubber was smaller than that of unmodified medical silicone rubber after 2 weeks and 6 weeks of implantation, and the statistical data of fiber cyst thickness at three time points were synthesized. The thickness of fiber capsule around modified silicone rubber decreased by about 35%, but compared with the original silicone rubber, the surface modified silicone rubber did not significantly reduce the inflammatory reaction.
【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08;TQ330.1

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