本文選題：骨組織工程 + 3D打印　； 參考：《中國人民解放軍醫(yī)學(xué)院》2017年碩士論文

【摘要】：研究背景及目的:長期以來,節(jié)段性骨缺損的修復(fù)一直未得到有效地解決,成為目前骨科醫(yī)生亟待解決的臨床難題。骨組織工程學(xué)的迅速發(fā)展為臨床骨缺損修復(fù)提供了新的思路。支架是骨組織工程最基本組成部分之一,如何構(gòu)建出理想的支架一直是骨組織工程領(lǐng)域研究的熱點。本研究基于不同材質(zhì),制備兩種新型多孔骨組織工程支架:3D打印多孔鈦支架(3D-STPT)及增強型BCP多孔陶瓷支架(RBCP);對兩種新型多孔骨組織工程支架的形貌和微觀結(jié)構(gòu)、孔徑和孔隙率、生物力學(xué)性能及生物組織相容性等方面進(jìn)行檢測;通過構(gòu)建兔前臂橈骨臨界性骨缺損模型對兩種新型多孔骨組織工程支架的骨缺損修復(fù)能力進(jìn)行初步評估。材料與方法:1、通過3D打印、表面化學(xué)處理、電化學(xué)沉積及雙氧水發(fā)泡法分別制備出3D打印多孔鈦支架(3D-STPT)及增強型BCP多孔陶瓷支架(RBCP),并對其形貌和微觀結(jié)構(gòu)、孔徑和孔隙率、生物力學(xué)性能等進(jìn)行評估。2、與MC3T3-E1細(xì)胞體外共同培養(yǎng),對兩種新型多孔骨組織工程支架進(jìn)行生物組織相容性評估,初步評估兩種新型多孔骨組織工程支架的生物組織相容性。3、通過構(gòu)建兔橈骨臨界性骨缺損模型對兩種新型多孔骨組織工程支架的骨缺損修復(fù)能力進(jìn)行初步評估。結(jié)果:1、SEM掃描結(jié)果顯示:兩種新型多孔骨組織工程支架均具備多孔結(jié)構(gòu),孔孔相連,孔徑及孔隙率:3D打印多孔鈦支架(3D-STPT)孔徑范圍為400μm-500μm,孔隙率為(66.0±0.7) %;增強型BCP多孔陶瓷支架(RBCP)孔徑范圍為100μm-500μm,孔隙率為(74.6±3.0) %,基本達(dá)到骨組織工程支架對孔徑及孔隙率基本要求(孔徑100 μm,孔隙率60%); X射線晶體衍射結(jié)果顯示:兩種新型多孔骨組織工程支架的表面成分與理論設(shè)計成分基本一致;生物力學(xué)性能測試結(jié)果顯示:3D打印多孔鈦支架(3D-STPT )壓縮強度為83.14±0.96MPa,增強型BCP多孔陶瓷支架(RBCP)壓縮強度為7.68±0.23MPa,兩種新型多孔骨組織工程支架符合骨組織工程支架生物力學(xué)性能標(biāo)準(zhǔn)。2、CCK-8結(jié)果顯示:兩種新型多孔骨組織工程支架能夠促進(jìn)MC3T3-E1細(xì)胞體外增殖,具有較好生物組織相容性。3、兔橈骨臨界性骨缺損修復(fù)實驗顯示:兩種新型多孔骨組織工程支架在動物體內(nèi)對臨界性骨缺損具有較好骨缺損修復(fù)作用。結(jié)論:1、基于兩種不同材質(zhì)和3D打印技術(shù),成功制備兩種新型多孔骨組織工程支架:3D打印多孔鈦支架(3D-STPT)和增強型BCP多孔陶瓷支架(RBCP),其孔徑大小、孔隙率、生物力學(xué)性能基本符合骨組織工程支架的要求;2、兩種新型多孔骨組織工程支架具有較好的生物組織相容性;3、兩種新型多孔骨組織工程支架能夠修復(fù)兔橈骨臨界性骨缺損,具有潛在的臨床應(yīng)用價值。
[Abstract]:Background and objective: for a long time, the repair of segmental bone defects has not been effectively resolved, and has become a clinical problem to be solved urgently by orthopedic doctors. The rapid development of bone tissue engineering provides a new idea for clinical bone defect repair. Scaffold is one of the most basic components of bone tissue engineering. In this study, two new porous bone tissue engineering scaffolds, 3D-STPT3D-STPT3D-STPTand enhanced BCP porous ceramic scaffolds were prepared based on different materials, and the morphology and microstructure, pore size and porosity of the two new porous bone tissue engineering scaffolds were investigated. The biomechanical properties and biocompatibility of the two new porous bone tissue engineering scaffolds were evaluated by constructing a critical bone defect model of rabbit forearm radius. Materials and methods: 1. 3D printed porous titanium scaffolds 3D-STPTand enhanced BCP porous ceramic scaffolds were prepared by 3D printing, surface chemical treatment, electrochemical deposition and hydrogen peroxide foaming, respectively, and their morphology and microstructure, pore size and porosity were analyzed. The biomechanical properties were evaluated. 2. The biocompatibility of two new scaffolds of porous bone tissue engineering was evaluated by co-culture with MC3T3-E1 cells in vitro. The biocompatibility of two new porous bone tissue engineering scaffolds was preliminarily evaluated. The ability of repairing bone defects of two new types of porous bone tissue engineering scaffolds was preliminarily evaluated by constructing a rabbit radius critical bone defect model. Results the SEM scanning results showed that the two new types of porous bone tissue engineering scaffolds had porous structures, and the holes were connected to each other. The pore size and porosity of 3D printed porous titanium scaffold 3D-STPTis 400 渭 m-500 渭 m and 66.0 鹵0.7 渭 m; the pore size range of enhanced BCP porous ceramic scaffold is 100 渭 m-500 渭 m and porosity is 74.6 鹵3.0), which is basically up to the basic pore size and porosity of bone tissue engineering scaffold. The results of X-ray crystal diffraction show that the surface composition of the two new porous bone tissue engineering scaffolds is basically the same as that of the theoretical design. The results of biomechanical properties test showed that the compression strength of 3D-STPT was 83.14 鹵0.96MPa, and the compressive strength of enhanced BCP porous ceramic scaffold was 7.68 鹵0.23MPa. two new porous bone tissue engineering scaffolds accord with the biomechanical strength of bone tissue engineering scaffolds. The results of CCK-8 showed that two new scaffolds of porous bone tissue engineering could promote the proliferation of MC3T3-E1 cells in vitro. The experimental results of rabbit radius critical bone defect repair showed that two new porous bone tissue engineering scaffolds had good effect on repairing critical bone defects in animals. Conclusion: 1. Based on two different materials and 3D printing technology, two new porous bone tissue engineering scaffolds, 3D-STPTT) and enhanced BCP porous ceramic scaffolds, were successfully prepared. The pore size and porosity of the scaffolds were measured. The biomechanical properties basically meet the requirements of bone tissue engineering scaffolds. The two new porous bone tissue engineering scaffolds have good biocompatibility, and two new types of porous bone tissue engineering scaffolds can repair the critical bone defect of rabbit radius. It has potential clinical application value.
【學(xué)位授予單位】：中國人民解放軍醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R318.08;R68

【參考文獻(xiàn)】

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