本文選題：角膜修復(fù)材料 + 殼聚糖　； 參考：《華南理工大學(xué)》2012年碩士論文

【摘要】：角膜維持其基本形態(tài)和功能，需要足夠的氧氣和營養(yǎng)物質(zhì)。但角膜缺乏血管，其所需物質(zhì)主要通過空氣、淚液和房水提供，，而這些營養(yǎng)物質(zhì)主要通過擴散作用進入角膜組織內(nèi)部。營養(yǎng)物質(zhì)能否通過角膜修復(fù)材料充足的運輸、擴散到角膜內(nèi)部，對材料的正常使用十分重要，因此，滲透性能是角膜修復(fù)材料的重要指標(biāo)。本研究以滲透擴散原理為基礎(chǔ)，設(shè)計安裝角膜修復(fù)材料滲透性能測定裝置，通過制備一系列角膜材料，利用自制裝置測定其對氧氣、葡萄糖、色氨酸、氯化鈉的滲透性，通過數(shù)據(jù)比較分析，研究材料性質(zhì)、混合結(jié)構(gòu)、混合比例等因素對角膜材料的滲透性能的影響，探討滲透性能的影響機理。 以氣相色譜儀作為核心的氧氣量檢測裝置，設(shè)計并安裝了符合角膜修復(fù)材料透氧性能測定要求的色譜法透氧系數(shù)檢測裝置。分別測定了低、中、高三種透氧性能的材料，通過與Illinois8003等壓法透氧儀所測定的數(shù)據(jù)比較說明，在10-15到10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)的范圍內(nèi)具有適用性和一定的準(zhǔn)確性。 根據(jù)池膜滲透法原理設(shè)計了滲透池裝置以測定溶液中小分子物質(zhì)在膜中的滲透系數(shù)，數(shù)據(jù)說明該裝置在測定葡萄糖、色氨酸、氯化鈉三種物質(zhì)在膜中的滲透系數(shù)時符合溶解——擴散原理和池膜滲透原理，具有一定的適用性和重復(fù)性。 制備了四種脫乙酰度的殼聚糖膜，并測定了膜對氧氣、葡萄糖、色氨酸、氯化鈉的滲透性能。脫乙酰度從77.1%增加到96.7%，殼聚糖的透氧系數(shù)從4.01×10-15mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)下降到3.74×10-15mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)，這主要是因為，隨著脫乙酰度的提高，分子鏈規(guī)整度提高導(dǎo)致膜的結(jié)晶度有所提高，膜的結(jié)構(gòu)趨于緊密，使氧氣的滲透性能降低。但是，隨著脫乙酰度的提高，殼聚糖膜的濕態(tài)透氧系數(shù)從0.73×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)提高到2.01×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)，葡萄糖的透過率從3.09×10~(-7)cm~2·s~(-1)提高到3.47×10~(-7)cm~2·s~(-1)，色氨酸的透過率從3.79×10~(-7)cm~2·s~(-1)提高到4.35×10~(-7)cm~2·s~(-1)，氯化鈉的透過率從8.33×10~(-7)cm~2·s~(-1)提高到9.12×10~(-7)cm~2·s~(-1)。這是因為，隨著脫乙酰度的提高，殼聚糖膜的平衡含水量提高，從而造成其物質(zhì)滲透性提高。 以EDC作為交聯(lián)劑，制備了殼聚糖-膠原復(fù)合膜，并測定了膜對氧氣、葡萄糖、色氨酸、氯化鈉的滲透性能。隨著膠原含量從0提高到45%，復(fù)合膜的濕態(tài)透氧系數(shù)從2.01×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)提高到5.41×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)，葡萄糖的滲透系數(shù)從3.47×10~(-7)cm~2·s~(-1)提高到10.67×10~(-7)cm~2·s~(-1)，色氨酸從4.2×10~(-7)cm~2·s~(-1)提高到12.45×10~(-7)cm~2·s~(-1)，氯化鈉從9.12×10~(-7)cm~2·s~(-1)提高到32.14×10~(-7)cm~2·s~(-1)。這主要是因為，隨著膠原用量的提高，復(fù)合膜的平衡含水量從48%提高到61%，從而提高了物質(zhì)在復(fù)合膜中的滲透率。隨著交聯(lián)劑EDC用量的提高，復(fù)合膜的滲透性能有所下降，這主要是因為，交聯(lián)劑的加入，提高了復(fù)合膜的交聯(lián)度，使復(fù)合膜結(jié)構(gòu)的緊密程度提高，降低了復(fù)合膜的平衡含水量，從而導(dǎo)致滲透性能降低。數(shù)據(jù)比較分析說明復(fù)合膜的滲透性能主要受其平衡含水量的影響。
[Abstract]:The cornea maintains its basic form and function, requiring sufficient oxygen and nutrients. But the cornea is lacking in blood vessels. The material is supplied mainly through air, tears, and aqueous humor. These nutrients are mainly through the diffusion into the cornea. Can the nutrients be transported to the cornea through the corneal repair material enough to spread into the cornea It is important for the normal use of materials. Therefore, permeability is an important indicator of corneal repair materials. Based on the principle of permeation and diffusion, this study designed and installed a device for determining the permeability of corneal repair materials. By preparing a series of corneal materials, it was used to determine the permeability of oxygen, glucose, tryptophan and sodium chloride. Through comparison and analysis of data, the influence of material properties, mixed structure, mixing ratio and other factors on the permeability of corneal materials is studied, and the mechanism of permeability is discussed.
Using the gas chromatograph as the core of the oxygen measuring device, a chromatographic method for detecting oxygen permeability coefficient is designed and installed. The materials of low, medium and high oxygen permeable properties are measured, respectively, by comparison with the data measured by the Illinois8003 isobaric oxygen permeable instrument, from 10-15 to 10-12m The applicability of LO_2 cm cm~ (-2) s~ (-1) Pa~ (-1) is applicable and certain accuracy.
According to the principle of cell membrane permeability method, a permeation pool device is designed to determine the permeability coefficient of medium and small molecular substance in the membrane. The data shows that the device conforms to the solubility of the three substances in the membrane of glucose, tryptophan and sodium chloride in the membrane, which has a certain applicability and repeatability.
Four deacetylation of chitosan membranes were prepared, and the permeability of the membrane to oxygen, glucose, tryptophan and sodium chloride was measured. The degree of deacetylation increased from 77.1% to 96.7%. The oxygen permeability coefficient of chitosan decreased from 4.01 x 10-15mLO_2. Cm~ (-2). S~ (-1). Pa~ (-1) to 3.74 * 10-15mLO_2. Cm. Cm~ (-2). With the increase of the degree of deacetylation and the increase of the molecular chain regularity, the crystallinity of the membrane is improved, the structure of the membrane tends to tighten and the permeability of oxygen is reduced. However, with the increase of the degree of deacetylation, the wet oxygen permeability coefficient of the chitosan film is increased from 0.73 * 10-12mLO_2. Cm. Cm~ (-2) to s~ (-1) Pa~ (-1) to 2.01 * 10-12mLO_2. Cm. Cm~ (-2). -1) Pa~ (-1), the transmittance of glucose increased from 3.09 x 10~ (-7) cm~2 s~ (-1) to 3.47 * 10~ (-7) cm~2 s~ (-7). The transmittance of tryptophan increased from 3.79 x to 4.35. The equilibrium water content of chitosan membrane increased, resulting in increased permeability of the material.
The chitosan collagen composite membrane was prepared with EDC as a crosslinker, and the permeability of the membrane to oxygen, glucose, tryptophan and sodium chloride was measured. With the increase of collagen content from 0 to 45%, the wet oxygen permeability coefficient of the composite membrane was increased from 2.01 * 10-12mLO_2. Cm. Cm~ (-1). Pa~ (-1) to 5.41 * 10-12mLO_2. Cm cm~. The osmotic coefficient of glucose increased from 3.47 x 10~ (-7) cm~2 / s~ (-1) to 10.67 x 10~ (-7) cm~2. S~ (-1), and tryptophan increased from 4.2 x 10~ (-7) to 32.14. The water content increased from 48% to 61%, thus increasing the permeability of the material in the composite membrane. With the increase of the crosslinking agent EDC, the permeability of the composite film decreased. This is mainly because the crosslinking agent is added to improve the crosslinking degree of the composite membrane, the tightness of the composite membrane structure is increased, the balance water content of the composite film is reduced, and the balance water content of the composite film is reduced. Data analysis shows that the permeability of the composite membrane is mainly affected by its equilibrium moisture content.

【學(xué)位授予單位】：華南理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08;TB39

【參考文獻】

相關(guān)期刊論文 前10條

1 劉麗;李佳戈;王建宇;王碩;湯京龍;陸頌芳;;軟性接觸鏡透氧系數(shù)測量方法研究[J];北京生物醫(yī)學(xué)工程;2007年01期

2 賴子尼;崔英德;嚴(yán)兆強;;PVA-海藻酸鈉-活性炭共聚物水凝膠氧氣滲透性能研究[J];材料導(dǎo)報;2008年04期

3 顏靜;高巖;王清;崔學(xué)軍;鐘雙玲;王萍;王洪艷;;明膠/聚乳酸復(fù)合纖維膜的制備、表征及作為角膜細(xì)胞載體的評價[J];高等學(xué)�；瘜W(xué)學(xué)報;2012年02期

4 黎新明,崔英德,蔡立彬;共聚物水凝膠接觸鏡材料的研究[J];高分子材料科學(xué)與工程;2004年02期

5 姚子昂,韓寶芹,劉偉治,劉萬順;殼聚糖-硫酸軟骨素共混膜性質(zhì)的研究[J];高技術(shù)通訊;2001年12期

6 閆濤;張明昌;;組織工程角膜支架材料再生絲素膜的生物相容性[J];國際眼科雜志;2008年08期

7 馬群;劉萬順;韓寶芹;楊朝忠;梁曄;;角膜組織工程支架殼聚糖基共混膜的制備及性能評價[J];功能材料;2010年09期

8 崔亦華,崔英德,黎新明,易國斌;角膜接觸鏡研究進展[J];廣州化工;2002年02期

9 譚幗馨;崔英德;周家華;易國斌;;鉀鈉離子在無規(guī)共聚物水凝膠中的滲透擴散行為[J];高分子材料科學(xué)與工程;2006年02期

10 黎新明,崔英德;水凝膠角膜接觸鏡材料及其應(yīng)用[J];化工進展;2002年10期

本文編號：1882697