本文選題：海藻酸鈣 + 生物相容性　； 參考：《青島大學(xué)》2016年碩士論文

【摘要】：目的研究以海藻酸鹽為代表的海洋生物提取物制備而成的醫(yī)用敷料的性能,分別從止血效果、生物相容性、抗菌性三方面內(nèi)容進(jìn)行分析,并與目前臨床上常用的普通紗布、明膠止血海綿等止血敷料作對照,探討以海藻酸鹽為原料制備的醫(yī)用敷料的臨床應(yīng)用價值。方法試驗(yàn)一,利用體外止血試驗(yàn)建立動物出血模型,將以上三種止血材料敷于新西蘭兔背部兩側(cè)創(chuàng)口。記錄出血時間,收集止血敷料計(jì)算止血前后質(zhì)量差值,以及用氰化高鐵血紅蛋白檢測試劑測止血后敷料洗滌下的血紅蛋白吸光度值,從以上三方面評價止血效果。試驗(yàn)二,先制備各受試敷料浸提液,再分別進(jìn)行體外溶血試驗(yàn)、急性全身毒性試驗(yàn)以及CCK-8體外細(xì)胞毒性試驗(yàn)。試驗(yàn)三,采用振蕩法評價敷料間對金黃色葡萄球菌的抗菌性差異。結(jié)果試驗(yàn)一,海藻酸鹽敷料、紗布、明膠止血海綿出血時間依次為95.28±3.95s、138.33±5.98s、101.25±4.53s(F=2433.23,P0.05),Hb光度吸收值依次為1.269±0.011、1.146±0.013、1.754±0.013(F=24764.804,P0.05),出血量依次為0.921±0.021g、0.900±0.012g、1.607±0.028g(F=3364.499,P0.05)。試驗(yàn)二,海藻酸鹽敷料浸提液的溶血率為0.8%;注射海藻酸鹽敷料浸提液的小鼠,癥狀較陰性對照組小鼠無明顯差異,沒有發(fā)生急性全身毒性反應(yīng);海藻酸鹽敷料的細(xì)胞毒性評價為1級;試驗(yàn)三,海藻酸鹽敷料的抗菌率為49%。結(jié)論海藻酸鹽纖維敷料止血速度快、能有效控制出血對象的出血量,止血效果好,綜合評價止血效果優(yōu)于明膠海綿與紗布;海藻酸鹽纖維敷料細(xì)胞毒性小、急性全身毒性反應(yīng)低、溶血率低,均符合國家規(guī)定的使用標(biāo)準(zhǔn),表現(xiàn)出有良好的生物相容性;海藻酸鹽纖維敷料對金黃色葡萄球菌的增殖有抑制作用且明顯優(yōu)于普通紗布,顯示良好的抗菌性。
[Abstract]:Objective to study the properties of medical dressings prepared from marine extracts represented by alginate, and to analyze the effects of hemostasis, biocompatibility and antimicrobial activity, and to compare them with common gauze, which are commonly used in clinical practice. Compared with gelatin hemostatic sponge and other hemostatic dressings, the clinical application value of medical dressings made from alginate was discussed. Methods in experiment 1, the animal bleeding model was established by in vitro hemostasis test, and the above three kinds of hemostatic materials were applied to the bilateral wounds in the back of New Zealand rabbits. The bleeding time was recorded, the quality difference before and after hemostasis was calculated by collecting the hemostatic dressing, and the hemoglobin absorbance value of the dressing washed after hemostasis was measured with cyanomethemoglobin test reagent, and the hemostatic effect was evaluated from the above three aspects. In the second experiment, the extracts of each dressing were prepared, and then hemolysis test, acute systemic toxicity test and CCK-8 cytotoxicity test in vitro were carried out respectively. In experiment 3, the difference of antibacterial activity between dressings against Staphylococcus aureus was evaluated by oscillating method. Results in experiment 1, the photometric absorption time of alginate dressing, gauze and gelatin hemostatic sponge was 95.28 鹵3.95s-1 138.33 鹵5.98sm 101.25 鹵4.53sm FU 2433.23P0.05Hb, respectively: 1.269 鹵0.0111.146 鹵0.0131.754 鹵0.013174.804, and the bleeding volume was 0.921 鹵0.021g / 0.900 鹵0.012g1.607 鹵0.028gF1. 607 鹵0.028gF1 / 3364.499P0.05A, respectively. The results showed that the photometric absorption time of alginate dressing, gauze and gelatin hemostatic sponge was 1.269 鹵0.0111.146 鹵0.0131.754 鹵0.013764.804 and 0.921 鹵0.021g / 0.900 鹵0.012g1.607 鹵0.028g / L respectively. In experiment 2, the hemolysis rate of the extract of alginate dressing was 0.8.The mice injected with alginate dressing had no significant difference in symptoms and had no acute systemic toxic reaction compared with the negative control group. The cytotoxicity evaluation of alginate dressing was grade 1, and the antibacterial rate of alginate dressing was 49%. Conclusion the alginate fiber dressing has the advantages of fast hemostatic speed, effective control of bleeding volume and good hemostatic effect, and the comprehensive evaluation of hemostatic effect is superior to that of gelatin sponge and gauze, and the cytotoxicity of alginate fiber dressing is less than that of gelatin sponge and gauze. The acute systemic toxicity was low, the hemolysis rate was low, all of them were in accordance with the national standard of use and showed good biocompatibility, the alginate fiber dressing had inhibitory effect on the proliferation of Staphylococcus aureus and was obviously superior to that of the common gauze. Show good antibacterial properties.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張林樸;王冠華;連小麗;李燕妮;代曉華;;海藻酸鈉/殼聚糖復(fù)合凝膠的制備與細(xì)胞毒性評價[J];中國組織工程研究;2014年21期

2 李麗;周建平;胡宇馳;;細(xì)胞濃度和作用時間對細(xì)胞毒性檢查法影響的探討[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2012年14期

3 彭中德;歐陽松山;;海藻酸鈉敷料止血性能的實(shí)驗(yàn)研究[J];中醫(yī)臨床研究;2012年11期

4 段曉杰;徐麗明;王春仁;;納米銀醫(yī)用抗菌敷料抗菌性能檢測方法的研究[J];藥物分析雜志;2012年01期

5 孫玉玲;劉杰;;醫(yī)用敷料用海藻纖維研究進(jìn)展[J];科技信息;2010年32期

6 郭興鋒;侯春林;張偉;魏長征;魏善建;;幾丁糖/海藻酸敷料止血性能的實(shí)驗(yàn)研究[J];生物骨科材料與臨床研究;2008年02期

7 展義臻;趙雪;王煒;;海藻酸/明膠共混纖維的制備與應(yīng)用[J];國際紡織導(dǎo)報(bào);2007年10期

8 秦益民;朱長俊;馮德明;周娟;王盼盼;;海藻酸鈣醫(yī)用敷料與普通棉紗布的性能比較[J];紡織學(xué)報(bào);2007年03期

9 奚廷斐;;生物醫(yī)用材料現(xiàn)狀和發(fā)展趨勢[J];中國醫(yī)療器械信息;2006年05期

10 劉盛輝,郎美東;新一代生物醫(yī)用材料[J];高分子通報(bào);2005年06期

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