γ射線照射和反復(fù)凍融對于肌腱生物力學(xué)的影響
發(fā)布時間:2018-05-11 03:30
本文選題:照射 + 生物力學(xué); 參考:《青島大學(xué)》2012年碩士論文
【摘要】:目的:分析γ射線照射和反復(fù)凍融處理對同種異體肌腱的生物力學(xué)性能的影響,為組織庫處理和保存同種異體肌鍵提供有價值的信息。 方法:在無菌操作下,從5具新鮮尸體上取40根指淺屈肌腱作為實驗材料,肌鍵隨機分為4組:1)新鮮組2)γ射線照射組3)反復(fù)凍融組4)γ射線+反復(fù)凍融組。每組肌腱10根,照射組肌鍵采用鉆60照射25kGy,凍融組肌鍵采用-80℃冷凍7天,25℃復(fù)融6小時,反復(fù)凍融4次。最后檢測肌腱的生物力學(xué)并與新鮮肌腱做比較。 結(jié)果:新鮮組與其他三組比較發(fā)現(xiàn)生物力學(xué)性質(zhì)明顯差異,主要表現(xiàn)在最大拉力、最大應(yīng)力及剛度。Y射線+反復(fù)凍融組在最大拉力、剛度和最大應(yīng)力方面,生物力學(xué)性質(zhì)與其他三組比較有明顯的下降。Y射線照射組與反復(fù)凍融組之間生物力學(xué)性能沒有顯著差異。 結(jié)論:在同種異體肌鍵的保存方面上,不建議應(yīng)用Y射線照射(25kGy)和反復(fù)凍融(4次)處理肌鍵,高劑量照射和凍融次數(shù)較多容易導(dǎo)致肌鍵生物力學(xué)性能下降,影響肌鍵使用壽命。
[Abstract]:Aim: to analyze the effects of 緯 -ray irradiation and repeated freezing and thawing on the biomechanical properties of allogeneic tendons, and to provide valuable information for tissue bank treatment and preservation of allogeneic muscle bonds. Methods: under aseptic operation, 40 flexor digitorum tendons were taken from 5 fresh cadavers as experimental materials. Muscle bonds were randomly divided into 4 groups: 1) fresh group (2) 緯 -ray irradiation group (3) repeated freezing and thawing group (4) 緯 -ray repeated freezing and thawing group. There were 10 tendons in each group. The muscle bonds in the irradiation group were irradiated with drill 60 at 25 kGy. In the freeze-thaw group, the muscle bonds were frozen at -80 鈩,
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