本文選題：陽(yáng)極氧化法 + TiO_2納米管��； 參考：《中國(guó)人民解放軍醫(yī)學(xué)院》2012年碩士論文

【摘要】：隨著現(xiàn)代醫(yī)學(xué)的快速發(fā)展，骨科手術(shù)中越來(lái)越多地應(yīng)用內(nèi)植物。內(nèi)植物植入人體后，一旦發(fā)生細(xì)菌感染，將可能導(dǎo)致內(nèi)植物感染。而內(nèi)植物感染是骨科手術(shù)后災(zāi)難性的并發(fā)癥，不僅會(huì)給患者造成巨大的身心痛苦和經(jīng)濟(jì)損失，也對(duì)骨科醫(yī)生提出了嚴(yán)峻的挑戰(zhàn)。因此，如何保證內(nèi)植物材料生物性能的同時(shí)還具備抑菌性或抗菌性成為骨科醫(yī)生急需解決的一個(gè)重要課題。 內(nèi)植物植入體內(nèi)后，瞬間被人體的體液包裹，，體液中的纖連蛋白也分布于內(nèi)植物表面，而纖連蛋白是細(xì)菌種植和粘附的主要生物學(xué)因素，由于細(xì)菌粘附是內(nèi)植物感染的始動(dòng)因素，因此對(duì)內(nèi)植物表面進(jìn)行抗纖連蛋白粘附修飾成為新的治療和預(yù)防策略。內(nèi)植物表面進(jìn)行超疏水修飾后，可以減少纖連蛋白對(duì)內(nèi)植物的粘附，即減少了細(xì)菌的種植和粘附，提高了內(nèi)植物的抑菌能力。此外，運(yùn)用電化學(xué)方法在內(nèi)植物表面裝載納米銀，利用銀的殺菌效應(yīng)可使內(nèi)植物具備一定的抗菌能力。 目的: 為了預(yù)防內(nèi)植物感染的發(fā)生，必須打破細(xì)菌在內(nèi)植物表面粘附的始動(dòng)環(huán)節(jié)。通過(guò)改變內(nèi)植物表面潤(rùn)濕性，對(duì)不同親疏水表面對(duì)纖連蛋白和細(xì)菌粘附影響作用機(jī)理進(jìn)行分析，并驗(yàn)證其抑菌效果。此外，在內(nèi)植物表面構(gòu)筑OCP（磷酸八鈣）/Ag/TiO_2復(fù)合結(jié)構(gòu)，并驗(yàn)證其抗菌效果。 材料和方法: 1.應(yīng)用電化學(xué)陽(yáng)極氧化法在鈦金屬表面構(gòu)建TiO_2納米管陣列薄膜 2.應(yīng)用自組裝法，將低表面能物質(zhì)修飾在TiO_2納米管陣列薄膜基礎(chǔ)上，在內(nèi)植物表面構(gòu)建超疏水涂層 3.應(yīng)用接觸角分析儀分析自組裝后內(nèi)植物超疏水涂層的潤(rùn)濕性 4.通過(guò)體外抗纖連蛋白粘附和體外模擬內(nèi)植物植入抑菌試驗(yàn)，比較不同表面對(duì)纖連蛋白和細(xì)菌粘附的影響，觀察內(nèi)植物超疏水表面的抗纖連蛋白粘附和抑菌效果 5.通過(guò)兩步電化學(xué)法在TiO_2納米管陣列膜層表面構(gòu)筑OCP/Ag/TiO_2復(fù)合結(jié)構(gòu) 6.通過(guò)體外抗菌性試驗(yàn)和動(dòng)物體內(nèi)植入試驗(yàn)等評(píng)價(jià)OCP/Ag/TiO_2復(fù)合涂層的抗菌性和生物活性 結(jié)果： 表面征貌：在氫氟酸溶液中，通過(guò)控制反應(yīng)時(shí)間、反應(yīng)電壓等參數(shù)，可在鈦金屬表面制備出一層形態(tài)相對(duì)規(guī)則、大體均勻一致管狀結(jié)構(gòu)，這層管狀結(jié)構(gòu)可形成叢林狀納米管陣列薄膜。 超疏水表面抑菌效果：經(jīng)低表面能物質(zhì)PETS修飾的TiO_2納米管陣列表面，其接觸角可達(dá)到156°±1.0°。體外抗纖連蛋白粘附和體外模擬內(nèi)植物植入抑菌試驗(yàn)證明：相對(duì)于親水和普通疏水表面，超疏水涂層能夠明顯降低纖連蛋白和細(xì)菌的粘附。 OCP/Ag/TiO_2復(fù)合涂層抗菌實(shí)驗(yàn)：體外實(shí)驗(yàn)證明，OCP/Ag/TiO_2復(fù)合涂層可以明顯減少細(xì)菌粘附，相對(duì)于空白對(duì)照組可減少50%。體內(nèi)實(shí)驗(yàn)證明，載有一定尺寸納米銀的OCP/Ag/TiO_2復(fù)合涂層可以明顯改善骨感染和骨髓炎臨床癥狀。病理結(jié)果：OCP/Ag/TiO_2復(fù)合涂層側(cè)的骨髓感染程度、皮質(zhì)骨破壞和骨膜反應(yīng)程度明顯低于對(duì)照側(cè)。 結(jié)論: 1.在鈦金屬表面可通過(guò)電化學(xué)陽(yáng)極氧化法構(gòu)建TiO_2納米管陣列薄膜。 2.通過(guò)自組裝法可在TiO_2納米管陣列表面構(gòu)成超疏水涂層，且該涂層能大大降低纖連蛋白和細(xì)菌的粘附。 3.在TiO_2納米管陣列表面，可以構(gòu)筑OCP/Ag/TiO_2納米復(fù)合結(jié)構(gòu)，該涂層不僅具有良好的生物相容性，而且對(duì)金黃色葡萄球菌表現(xiàn)出優(yōu)良的抗菌性。
[Abstract]:With the rapid development of modern medicine, more and more internal plants are used in Department of orthopedics surgery. Once the internal plants are implanted in the human body, once the bacterial infection occurs, the infection of the internal plants will lead to the infection of the internal plants. Therefore, how to ensure the biological properties of the internal plant materials as well as bacteriostasis or antibacterial property has become an important issue that the Department of orthopedics doctors need to solve urgently.
After the internal plant is implanted in the body, it is instantly wrapped in body fluid, and fibronectin in the body fluid is also distributed on the surface of the plant. Fibronectin is the main biological factor for the cultivation and adhesion of bacteria. As the adhesion of bacteria is the starting factor of the internal plant infection, the adhesion modification of anti fibronectin to the surface of the internal plants becomes a new treatment. And the prevention strategy. After superhydrophobic modification, the surface of the plant can reduce the adhesion of fibronectin to the internal plants, that is, reducing the planting and adhesion of bacteria and improving the bacteriostasis ability of the internal plants. In addition, the use of electrochemical methods to load the nano silver on the surface of plants and the bactericidal effect of silver can make the internal plants have certain antibacterial energy. Power.
Objective:
In order to prevent the occurrence of internal plant infection, it is necessary to break the starting link of the surface adhesion of plants. By changing the wettability of the surface of the plant, the mechanism of the effects of different water meters on fibronectin and bacterial adhesion is analyzed, and its bacteriostasis effect is verified. In addition, the surface of the plant is constructed of OCP (eight calcium phosphate) /Ag/TiO_2 The composite structure is used to verify its antibacterial effect.
Materials and methods:
1. construction of TiO_2 nanotube array film on titanium surface by electrochemical anodization
2. self assembly method was used to modify low surface energy materials on the TiO_2 nanotube array film and build super hydrophobic coating on the surface of the plant.
3. contact angle analyzer was used to analyze the wettability of the superhydrophobic coating after self assembly.
4. the effects of different surfaces on the adhesion of fibronectin and bacteria were compared by adhesion of antifibronectin in vitro and in vitro simulated plant implantation, and the adhesion and bacteriostasis effect of antifibronectin on the superhydrophobic surface of the plant was observed.
5. two step electrochemical method was used to construct OCP/Ag/TiO_2 composite structure on the surface of TiO_2 nanotube arrays.
6. the antibacterial activity and bioactivity of OCP/Ag/TiO_2 composite coating were evaluated by in vitro antibacterial test and in vivo implantation test.
Result錛

本文編號(hào)：1862765