發(fā)布時間：2018-04-28 21:11

  本文選題：羥基磷灰石 + 有機分子　； 參考：《暨南大學》2012年碩士論文

【摘要】：在生物材料研究領(lǐng)域中，羥基磷灰石（HAP）由于其化學成分結(jié)構(gòu)與骨牙中的無機成分高度相似，在硬組織替換與修復(fù)領(lǐng)域中得到廣泛的應(yīng)用。生物礦化形成的自然骨和牙中HAP的形貌分別為納米片狀和六方棒狀，與常規(guī)人工合成HAP形態(tài)差異較大。 本研究基于生物礦化的基本原理，采用水熱反應(yīng)法，以富含氨基和羧基的有機小分子甘氨酸，乙二胺，谷氨酸，檸檬酸鈉為模型分子，研究小分子基團對HAP的晶體生長形貌的影響；探討不同形貌HAP的制備方法和形成機理。采用透射電子顯微鏡（TEM）、X-射線衍射儀（XRD）、拉曼（Laman）和傅立葉變換紅外光譜儀（FTIR）分析了合成產(chǎn)物的形貌、結(jié)構(gòu)和組成。實驗結(jié)果表明：乙二胺的誘導下合成了寬30～50nm，長寬比3～5的片狀HAP晶體；甘氨酸誘導合成的HAP呈六方棒狀，長500～1500nm，直徑50～100nm；谷氨酸誘導合成的HAP由納米棒狀組裝成的片狀結(jié)構(gòu)，長500～2000nm，寬50～200nm。而檸檬酸鈉誘導合成的HAP也呈片狀，長200～500nm，寬20～50nm，尺寸均勻。對照空白實驗可知，小分子上的極性基團通過靜電吸附或分子占位顯著影響HAP的形貌。帶正電的氨基可能占據(jù)Ca2+的空缺位置，特別是在（001）面，故乙二胺調(diào)控下，HAP在(001)面的生長受到抑制，形成片狀。帶負電荷的羧基與Ca2+強烈的配位作用及分子間的相互作用，導致甘氨酸、谷氨酸和檸檬酸分子形成了不同的形貌HAP。在谷氨酸分子調(diào)控下，納米棒HAP（100）面的Ca2+吸附羧基，谷氨酸在（100）面形成C—N—C分子層，通過羧基與Ca2+的作用組裝成納米有序排列的片狀HAP晶體。含有3個羧基的檸檬酸與Ca2+的強配位作用，防止了HAP的厚化，，使其保持片狀形貌。該研究從一定角度證實了自然骨形成過程中檸檬酸干擾骨質(zhì)磷灰石的形成穩(wěn)定其片狀結(jié)構(gòu)可能存在的調(diào)控機制。 本研究對上述不同形貌的納米HAP材料的生物相容性進行了研究。通過顆粒懸液檢測材料的細胞毒性，結(jié)果表明所合成的幾種不同形貌的納米HAP材料隨著顆粒濃度的提高，細胞的活性降低。當顆粒濃度達到10μg/ml以下，沒有表現(xiàn)出明顯的細胞毒性。在檢測濃度范圍內(nèi)（50～5μg/ml），納米晶粒形貌的不同未導致細胞活性的差異。研究了納米HAP對成骨細胞的增殖、ALP的表達和細胞形態(tài)的影響。從MTT統(tǒng)計結(jié)果表明，隨著納米HAP濃度的增加，成骨細胞活性逐漸下降。納米顆粒尺寸增大，成骨細胞活性下降。納米HAP抑制了成骨細胞的生長，促進成骨細胞ALP的表達。SEM和激光共聚焦顯微鏡的結(jié)果表明不同樣品對細胞形態(tài)的影響并沒有明顯的差異。
[Abstract]:Hydroxyapatite (HAP) has been widely used in the field of hard tissue replacement and repair because of its high similarity in chemical composition and inorganic composition in bone teeth in the field of biomaterial research. The morphology of HAP in the natural bone and teeth formed by biomineralization was nanoscale and hexagonal, which was different from that of conventional synthetic HAP. Based on the principle of biomineralization, the model molecules of glycine, ethylenediamine, glutamic acid and sodium citrate, which are rich in amino and carboxyl groups, were used in this study. The effects of small molecular groups on the growth morphology of HAP were studied, and the preparation methods and formation mechanism of HAP with different morphologies were discussed. The morphology, structure and composition of the synthesized products were characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). The experimental results showed that HAP crystals with width of 30 ~ 50 nm and aspect ratio of 3 ~ 5 were synthesized under the induction of ethylenediamine, HAP synthesized by glycine was hexagonal, 500 ~ 1 500 nm in length and 50 ~ 100 nm in diameter, and HAP synthesized by glutamate was composed of nanorods of 50 000 nm in length and 50 ~ 200 nm in width. The HAP induced by sodium citrate was also flake, with a length of 200 ~ 500 nm and a width of 20 ~ 50 nm with a uniform size. Compared with the blank experiments, the polar groups on the small molecules have significant influence on the morphology of HAP by electrostatic adsorption or molecular occupation. The positively charged amino group may occupy the vacant position of Ca2, especially on the surface of Ca2. Therefore, the growth of Ca2 on the surface of Ca2 is inhibited under the control of ethylenediamine. The strong coordination and intermolecular interaction between negatively charged carboxyl groups and Ca2 lead to the formation of different morphologies of glycine, glutamate and citric acid molecules. Under the control of glutamate molecule, Ca2 adsorbs carboxyl group on the surface of nanorod HAP-100, and glutamic acid forms C-N-C molecular layer on the surface of HAP-100). Through the interaction of carboxyl group and Ca2, the ordered sheet HAP crystal is assembled. The strong coordination of citric acid containing three carboxyl groups with Ca2 prevents the thickening of HAP and keeps it flake. This study confirmed the possible regulatory mechanism of citric acid interfering with the formation of bone apatite in the process of natural bone formation in order to stabilize its lamellar structure. The biocompatibility of nanometer HAP materials with different morphologies was studied in this paper. The cytotoxicity of the material was detected by particle suspension. The results showed that the cell activity of several nano-sized HAP materials with different morphologies decreased with the increase of particle concentration. When the particle concentration was below 10 渭 g/ml, there was no obvious cytotoxicity. In the concentration range of 50 渭 g / ml, the difference of the morphology of nanocrystalline did not lead to the difference of cell activity. The effects of nanometer HAP on the expression and morphology of osteoblasts were studied. The results of MTT showed that the activity of osteoblasts decreased with the increase of HAP concentration. The size of nanoparticles increased and the activity of osteoblasts decreased. Nanometer HAP inhibited the growth of osteoblasts and promoted the expression of ALP in osteoblasts. The results of laser confocal microscopy showed that there was no significant difference in the effect of different samples on the morphology of osteoblasts.
【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.08;TB383.1

【參考文獻】

相關(guān)期刊論文 前10條

1 孫衛(wèi)斌,吳亞菲,丁一,儲成林,劉天佳;納米羥基磷灰石對人牙周膜細胞增殖活性的影響[J];東南大學學報(自然科學版);2004年06期

2 李麗麗,劉陽,褚瑩;表面活性劑對納米CaCO_3形貌的調(diào)控[J];分子科學學報;2005年01期

3 武麗華;張光華;殷海榮;陳福;;溶膠-凝膠法制備生物活性有機-無機雜化材料的研究進展[J];硅酸鹽通報;2007年01期

4 馬維平;孫洪巍;苗芳;王松;胡行;;十六烷基三甲基溴化銨/正丁醇/環(huán)己烷/水微乳液制備納米粉體的研究[J];硅酸鹽通報;2008年03期

5 吳苑;席詠梅;劉玉侃;趙小立;姚行;張銘;;納米羥基磷灰石顆粒對成骨前體細胞的增殖和分化影響[J];浙江大學學報(理學版);2008年06期

6 王靜康,黃向榮,劉秉文,張纓;有機分子晶體晶習預(yù)測的研究進展[J];人工晶體學報;2002年03期

7 李廣慧,韓麗,方奇;晶體結(jié)構(gòu)控制晶體形態(tài)的理論及應(yīng)用[J];人工晶體學報;2005年03期

8 鄔鴻彥;HAP的結(jié)構(gòu)及物理性能[J];四川師范大學學報(自然科學版);1996年05期

9 胡耀武,王昌燧,左健,張玉忠;古人類骨中羥磷灰石的XRD和喇曼光譜分析[J];生物物理學報;2001年04期

10 王靜梅,姚松年;殼聚糖-氨基酸體系中碳酸鈣模擬生物礦化的研究[J];無機化學學報;2002年03期

本文編號：1816886