本文選題：流式細(xì)胞儀 + 低流速�。� 參考：《天津大學(xué)》2012年碩士論文

【摘要】：流式細(xì)胞儀作為一種新型的細(xì)胞定量分析和分選儀器，能夠在功能層面上對(duì)單個(gè)細(xì)胞進(jìn)行快速多參數(shù)分析，但其形態(tài)學(xué)信息檢測(cè)能力略顯不足。本課題組研究的散射成像式流式細(xì)胞儀正好彌補(bǔ)這一不足，該系統(tǒng)基于成像原理，用CCD記錄與細(xì)胞形態(tài)信息相關(guān)的散射圖像。實(shí)驗(yàn)證明利用獲得的散射圖像能夠識(shí)別淋巴B細(xì)胞與T細(xì)胞，對(duì)細(xì)胞形態(tài)學(xué)研究具有重要意義，然而受相機(jī)快門(mén)速度的限制，亟需開(kāi)發(fā)一種適合成像的低流速液流系統(tǒng)。本課題正是基于這一目的從流體室設(shè)計(jì)、液流驅(qū)動(dòng)系統(tǒng)設(shè)計(jì)以及鞘流壓強(qiáng)PID控制三個(gè)方面進(jìn)行深入研究。 1、流體室是流式細(xì)胞儀的核心部件，，對(duì)儀器的功能實(shí)現(xiàn)具有重大影響，本論文按照流式細(xì)胞儀流動(dòng)單細(xì)胞測(cè)量的特點(diǎn)，進(jìn)行了流體室設(shè)計(jì)、流體運(yùn)動(dòng)狀態(tài)分析、最佳檢測(cè)區(qū)位置選擇，并結(jié)合COMSOL Mutiphysics軟件模擬結(jié)果與實(shí)驗(yàn)效果，對(duì)流體室結(jié)構(gòu)進(jìn)行改進(jìn)。 2、液流驅(qū)動(dòng)系統(tǒng)是液流系統(tǒng)的重要組成部分，其性能直接影響流體室內(nèi)核流穩(wěn)定性。在系統(tǒng)開(kāi)發(fā)過(guò)程中先后設(shè)計(jì)并試驗(yàn)多種驅(qū)動(dòng)方案，包括重力、針管泵、壓強(qiáng)等多種形式，并在大量實(shí)驗(yàn)基礎(chǔ)上，綜合考慮核流形狀穩(wěn)定性、壓縮直徑、流速等因素，最終確定氣壓驅(qū)動(dòng)鞘流、針管泵驅(qū)動(dòng)核流的方案。 3、論文設(shè)計(jì)了以單片機(jī)、注氣泵、壓強(qiáng)傳感器構(gòu)成反饋回路的數(shù)字PID閉環(huán)負(fù)反饋系統(tǒng)，并進(jìn)行參數(shù)整定。實(shí)驗(yàn)結(jié)果顯示：該低流速液流系統(tǒng)能夠在流速低至5mm/s時(shí)，將核流直徑壓縮到30μm以下，且核流形狀穩(wěn)定，能夠滿足散射成像式流式細(xì)胞儀的成像需求。
[Abstract]:As a new type of cell quantitative analysis and sorting instrument, flow cytometry (FCM) can analyze a single cell quickly and multi-parameter on the functional level, but its ability of morphological information detection is a little insufficient. The scattering imaging flow cytometry (FCM) studied by our team can make up for this deficiency. Based on the imaging principle, the system records scattering images related to cell morphological information by CCD. Experimental results show that the scattering images can recognize lymphoid B cells and T cells, which is of great significance to the study of cell morphology. However, due to the limitation of camera shutter velocity, it is urgent to develop a low velocity liquid flow system suitable for imaging. The purpose of this paper is to study the design of fluid chamber, the design of fluid flow drive system and the PID control of sheath flow pressure. 1. The fluid chamber is the core component of the flow cytometry, which has great influence on the realization of the function of the instrument. According to the characteristics of flow cytometry single cell measurement, the fluid chamber is designed and the fluid motion state is analyzed. The structure of the fluid chamber was improved by selecting the best location of the detection area and combining the simulation results with the experimental results of COMSOL Mutiphysics software. 2. Liquid flow drive system is an important part of liquid flow system, and its performance directly affects the stability of nuclear flow in fluid chamber. In the course of system development, many kinds of driving schemes were designed and tested successively, including gravity, needle pump, pressure and so on. On the basis of a large number of experiments, the stability of nuclear flow shape, compression diameter, flow rate and other factors were considered synthetically. Finally, the scheme of pneumatic driven sheath flow and needle pump driven nuclear flow was determined. 3. A digital PID closed loop negative feedback system composed of single chip microcomputer, air pump and pressure sensor is designed, and the parameters are adjusted. The experimental results show that the low velocity liquid flow system can compress the diameter of the nuclear flow below 30 渭 m when the flow velocity is lower than 5mm/s, and the shape of the nuclear flow is stable, which can meet the imaging requirements of scattering imaging flow cytometry.
【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.6

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本文編號(hào)：1791052