發(fā)布時(shí)間：2018-04-22 10:19

  本文選題：機(jī)械式同軸擠出沉積成型 + 組織工程骨支架　； 參考：《新疆大學(xué)》2017年碩士論文

【摘要】：針對由結(jié)核桿菌侵入骨骼或關(guān)節(jié)引起的骨結(jié)核病變,為提高病灶區(qū)域的藥物利用度,達(dá)到高效治療骨結(jié)核病及快速修復(fù)骨缺損并進(jìn)的雙重目的,將快速成形技術(shù)(RP)、組織工程技術(shù)(TE)與生物載藥相結(jié)合,構(gòu)建以組織工程骨支架為基礎(chǔ)的植入式載藥緩釋系統(tǒng)。為實(shí)現(xiàn)骨結(jié)核病藥物緩釋治療兼具個性化骨缺損修復(fù)提供了全新的思路和有效的方法。本學(xué)位論文以同軸復(fù)合材料組織工程骨支架的材料選擇與同軸結(jié)構(gòu)構(gòu)建為關(guān)鍵出發(fā)點(diǎn),構(gòu)建同軸3D打印試驗(yàn)平臺與設(shè)計(jì)同軸噴頭擠出裝置,研究絲素蛋白(SF)與聚乙烯醇(PVA)的最優(yōu)配比制備SF/PVA復(fù)合水凝膠,并與羥基磷灰石(HA)混合制備SF/PVA/HA復(fù)合材料作為同軸支架的內(nèi)芯增韌材料,同時(shí)選用PVA/HA復(fù)合材料作為同軸支架的殼層材料。采用機(jī)械式同軸擠出快速成形技術(shù)制備同軸支架,分析同軸支架打印過程的各工藝參數(shù),研究材料調(diào)配比例、同軸噴頭比例、掃描速度、擠出速度等因素對同軸支架成形精度的影響,優(yōu)化關(guān)鍵工藝參數(shù)值,制備多種材料復(fù)合、宏觀形貌良好、孔隙結(jié)構(gòu)均勻且絲材同軸度高的同軸復(fù)合材料組織工程骨支架。針對成形支架的成骨潛能、生物相容性進(jìn)行了細(xì)胞毒性實(shí)驗(yàn),證明了本文研究結(jié)果的可行性和有效性,并通過成形支架的體外降解實(shí)驗(yàn)及動物回植實(shí)驗(yàn)驗(yàn)證了支架的降解速率及成骨效能,為以組織工程骨支架為基礎(chǔ)的植入式載藥緩釋系統(tǒng)的研究和應(yīng)用提供了一定的實(shí)驗(yàn)依據(jù)。
[Abstract]:In order to improve the drug availability of bone tuberculosis caused by the invasion of tuberculosis bacillus into bone or joint, to achieve the dual purpose of treating bone tuberculosis disease and repairing bone defect quickly. The rapid prototyping (RP) and tissue engineering (TEC) were combined with biological agents to construct an implantable drug delivery system based on tissue engineering bone scaffolds. It provides a new idea and effective method for the treatment of bone tuberculosis with drug release and individualized bone defect repair. Based on the material selection and coaxial structure construction of the coaxial composite tissue engineering bone scaffold, the coaxial 3D printing test platform and the coaxial nozzle extrusion device are constructed in this dissertation. SF/PVA composite hydrogels were prepared by the optimal ratio of silk fibroin (SF) and polyvinyl alcohol (PVA), and SF/PVA/HA composites were mixed with hydroxyapatite (HA) as core toughening materials for coaxial scaffolds. At the same time, PVA/HA composite is used as the shell material of coaxial support. Coaxial bracket was prepared by mechanical coaxial extrusion rapid prototyping technology. The process parameters of coaxial support printing process were analyzed, and the proportion of material blending, coaxial nozzle ratio and scanning speed were studied. The influence of extrusion speed on the forming accuracy of coaxial scaffold was studied. The key process parameters were optimized and many kinds of composite materials were prepared. The microstructure of coaxial composite scaffolds with good macroscopic morphology uniform pore structure and high coaxiality of filaments were obtained. According to the osteogenic potential and biocompatibility of the scaffold, a cytotoxicity test was carried out, which proved the feasibility and effectiveness of the present study. The degradation rate and osteogenic efficiency of the scaffold were verified by in vitro degradation experiments and animal replantation experiments, which provided a certain experimental basis for the research and application of implantable drug delivery sustained release system based on tissue engineering bone scaffold.
【學(xué)位授予單位】：新疆大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R318.08

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