本文選題：室溫固化型基托樹脂 + 加熱固化型基托樹脂��； 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]檢測三種基托樹脂的細胞毒性,評價其生物相容性,為臨床材料使用提供依據(jù)。[方法]采用SRB法、流式細胞儀法檢測加熱固化型、室溫固化型、光固化型三種基托樹脂3天、7天浸提液對小鼠胚胎成纖維細胞L929的3天、7天細胞增殖率、細胞凋亡率的影響,評價比較三種基托樹的體外細胞毒性。[結(jié)果]1.室溫固化型基托樹脂:細胞增值率:反應3天:3天浸提液為58.6%,7天浸提液為66.9%;細胞增殖0D值比較有統(tǒng)計學意義(P0.05)。反應7天:3天浸提液為61.3%,7天浸提液為57.4%;細胞增值0D值比較有統(tǒng)計學意義(P0.05)。細胞凋亡率:反應3天:3天浸提液為4.4%,7天浸提液為7.0%;反應7天:3天浸提液為5.1%,7天浸提液為5.5%。2.加熱固化型基托樹脂:細胞增值率:反應3天:3天浸提液為78.3%;7天浸提液為76.3%;細胞增殖OD比較無統(tǒng)計學意義(P0.05)。反應7天:3天浸提液為76.4%;7天浸提液為75.7%;細胞增殖OD比較無統(tǒng)計學意義(P0.05)。細胞凋亡率:反應3天:3天浸提液為3.1%;7天浸提液為5.9%;反應7天:3天浸提液為3.1%;7天浸提液為3.3%。3.光固化型基托樹脂:細胞增殖率:反應3天:3天浸提液為90.2%;7天浸提液為89.1%;細胞增殖OD比較無統(tǒng)計學意義(P0.05)。反應7天:3天浸提液為90.0%;7天浸提液為88.8%;細胞增殖OD比較無統(tǒng)計學意義(P0.05)。細胞凋亡率:反應3天:3天浸提液為2.2%;7天浸提液為4.7%;反應7天:3天浸提液為2.8%;7天浸提液為2.5%。4.三種基托樹脂:細胞增值OD值:反應3天:比較有統(tǒng)計學意義(P0.01),其中兩兩相較均具有統(tǒng)計學意義。反應7天:比較有統(tǒng)計學意義(P0.01),其中兩兩相較均具有統(tǒng)計學意義。[結(jié)論]1.室溫固化型基托樹脂細胞毒性為2級,提示對細胞影響較大。2.加熱固化型基托樹脂細胞毒性為1級,提示對細胞影響較小。3.光固化型基托樹脂細胞毒性為1級,提示對細胞影響較小。
[Abstract]:Objective: to detect the cytotoxicity of three base resins and evaluate their biocompatibility.[methods] SRB assay and flow cytometry were used to detect the effects of heat curing, room temperature curing and light curing on the proliferation rate and apoptosis rate of mouse embryonic fibroblasts L929 at 3 days and 7 days.To evaluate and compare the cytotoxicity of three kinds of basilar trees in vitro.[result] 1.Room temperature curing base resin: cell proliferation rate: the ratio of cell proliferation was 58.6% in 3 days and 66.9 in 7 days, and the 0D value of cell proliferation was significantly higher than that of P0.05.On the 7th day of reaction, 61.3% of the extract was 61.3% and 57.4% on the 7th day, and the value of cell increment 0D was significantly higher than that of P0.05%.The apoptotic rate was 7.0 in the solution of 4. 4% and 7 days in the 3 days of reaction, and 5. 1 and 5. 2 in the solution of 5. 1 and 7 days after 7 days of reaction.Heating curing base resin: cell proliferation rate: the ratio of cell proliferation was 78.3% in 3 days and 78.3% in 7 days, and the OD ratio of cell proliferation was not significant (P 0.05).The ratio of cell proliferation OD to 75.7 was 75.7 in 7 days and 75.7 in 7days, and there was no significant difference in OD value of cell proliferation.The apoptotic rate was 3 days, 3 days, 3 days, 3 days, 7 days, 7 days, 3 days, 3 days, 3 days, 7 days, and 7 days, 3 days, 3 days, 3 days, 7 days, and 7 days, 3. 1 and 3. 5%, 3. 1%, 3. 3%, 3. 9%, 3. 9%, 3. 9%.Light curing base resin: cell proliferation rate: the ratio of cell proliferation was 90.2% in 3 days and 89.1 in 7 days, and the OD ratio of cell proliferation was not significant (P 0.05).The ratio of cell proliferation OD was not statistically significant (P 0.05), and the ratio of cell proliferation OD was 88. 8% in 7 days and 90. 0% in 7 days, and 90. 0% in 7 days, and 88. 8 in 7 days.The apoptotic rate was 2.7% in the solution of 2. 2% and 2. 7% in 3 days and 2. 5% in 7 days, and 2. 5% in 7 days, and 2. 5% in 7 days, and 2. 5% in 7 days, and 2. 5% in 7 days and 2. 5%. 4 in 7 days, and 2. 8% in 7 days, respectively.Three kinds of base resin: OD value of cell increment: reaction 3 days: the comparison was statistically significant (P 0.01), in which the two phases were statistically significant.The response was 7 days: there was statistical significance in the comparison of P 0.01 and P 0. 01, in which the pairings were statistically significant.[conclusion] 1.The cytotoxicity of the curing base resin at room temperature was 2 grade, indicating that the effect on the cell was greater. 2. 2.The cytotoxicity of the heat-cured base resin was grade 1, indicating that the effect on the cells was small. 3.The cytotoxicity of light curing base resin was grade 1, suggesting that it had little effect on cells.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R783.1

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