本文選題：光固定 + 明膠��； 參考：《吉林大學(xué)》2013年博士論文

【摘要】：PLGA是一種無毒可降解的高分子有機(jī)聚合物，其在機(jī)體內(nèi)代謝首先通過聚酯水解為乳酸和羥基乙酸，隨后被完全分解成為二氧化碳和水排出體外，生物相容性好。PVA水凝作為人工敷料的優(yōu)點(diǎn)是含水量豐富，不但可以保持創(chuàng)面濕潤，還具有吸水性可以吸收組織滲出液，同時其內(nèi)部大量貫通的孔隙有利于營養(yǎng)物質(zhì)和代謝廢物的運(yùn)輸，且又鎮(zhèn)痛作用，因而廣泛應(yīng)用與組織工程領(lǐng)域。本研究綜合了兩者的優(yōu)勢，并采用物理交聯(lián)的方式將二者復(fù)合，構(gòu)建一種類似皮膚結(jié)構(gòu)的雙層人工支架，使之更適合于臨床骨皮膚損傷、修復(fù)的需要。 明膠提取自動物的骨頭或結(jié)締組織，無毒無害，被廣泛用于食品、制藥及組織工程領(lǐng)域。在皮膚組織工程領(lǐng)域研究中，明膠除了具有促進(jìn)細(xì)胞粘附和生長的作用，還可以作為一種底物基質(zhì)與細(xì)胞外基質(zhì)產(chǎn)生競爭作用，防止傷口部位的蛋白酶消化細(xì)胞外基質(zhì)。明膠分子結(jié)構(gòu)上含有大量的羥基及少量的羧基和氨基，具有極強(qiáng)的親水性。因此，明膠分子中的氨基可以與光活性疊氮基團(tuán)發(fā)生縮合反應(yīng)，形成酯類。本研究為進(jìn)一步提高人工皮膚支架材料的生物活性，以組織工程生物材料為基底，探討光固定表面改性的對其生物相容性的影響。 結(jié)果顯示：本研究利用光活性疊氮基團(tuán)與明膠分子的羧基發(fā)生了縮合反應(yīng)，形成具有光活性的新的化合物—Az-G光活性明膠；通過在材料表面的固定效果和細(xì)胞學(xué)評價，證明Az-G光活性明膠可以穩(wěn)定的固定在生物材料表面，不會脫落，且細(xì)胞相容性好，有利于細(xì)胞粘附、生長和增殖；本研究首次將靜電紡絲膜和PVA水凝膠通過物理冷凍交聯(lián)的方法結(jié)合，，其機(jī)型性能和保水性遠(yuǎn)優(yōu)于單純的PLGA電紡絲膜或PVA水凝膠；采用模具法制備的“多孔”PVA水凝膠有利于細(xì)胞的粘附和生長。
[Abstract]:PLGA is a nontoxic and degradable polymeric organic polymer. Its metabolism is first hydrolyzed into lactic acid and glycolic acid by polyester and then completely decomposed into carbon dioxide and water.Good biocompatibility. PVA hydration as artificial dressing has the advantage of abundant water content, which not only keeps the wound moist, but also absorbs the tissue exudate.At the same time, a large number of perforated pores are conducive to the transport of nutrients and metabolic waste, and analgesic effect, so they are widely used in the field of tissue engineering.In this study, the advantages of both were synthesised, and they were combined by physical crosslinking to construct a kind of double-layer artificial scaffold similar to skin structure to make it more suitable for clinical bone skin injury and repair.Gelatin is extracted from animal bone or connective tissue. It is nontoxic and harmless. It is widely used in food, pharmaceutical and tissue engineering fields.In the field of skin tissue engineering, gelatin can not only promote cell adhesion and growth, but also act as a substrate to compete with extracellular matrix and prevent protease from digesting extracellular matrix.Gelatin contains a large number of hydroxyl groups, a small amount of carboxyl and amino groups, and has a strong hydrophilicity.Therefore, amino groups in gelatin molecules can be condensed with photoactive azide groups to form esters.In order to further improve the bioactivity of artificial skin scaffolds, the effect of surface modification on biocompatibility of tissue engineering biomaterials was investigated.The results showed that the photoactive azide group reacted with the carboxyl group of gelatin molecule to form a new photoactive compound -Az-G photoactive gelatin.The results showed that Az-G photoactive gelatin could be stably immobilized on the surface of biomaterials without falling off, and had good cell compatibility, which was beneficial to cell adhesion, growth and proliferation.In this study, the electrospun film and PVA hydrogel were combined by physical freezing crosslinking for the first time. The performance and water retention of the electrospun membrane and PVA hydrogel were much better than those of PLGA electrospinning film or PVA hydrogel.The "porous" PVA hydrogel prepared by mould method is beneficial to cell adhesion and growth.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2013
【分類號】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 鄒勃生,譚軍,李效,葉倩;青蛙皮及乳豬皮治療深度燒傷創(chuàng)面的比較研究[J];中國醫(yī)師雜志;2004年01期

2 趙劍豪;屠美;田冶;王玨;;等離子體處理偶聯(lián)膠原提高聚乳酸材料的細(xì)胞相容性[J];高分子材料科學(xué)與工程;2008年07期

3 龐建華,楊嵩峰,陳偉華,張毓?jié)?郭錦平,劉曉薇,楊宇梓;復(fù)層輻射人羊膜應(yīng)用于燒傷創(chuàng)面的研究[J];黑龍江醫(yī)學(xué);2001年02期

4 沈光裕;潘銀根;陳建設(shè);周金武;段紅杰;陳琦;李鴻明;;豬脫細(xì)胞真皮基質(zhì)在人皮膚創(chuàng)傷修復(fù)中的臨床應(yīng)用[J];華南國防醫(yī)學(xué)雜志;2010年02期

5 王文文;陶慧敏;李志斌;陳佳媚;廖瑞雪;郭浩然;關(guān)燕清;;共固定化TNF-α/IFN-γ對宮頸癌HeLa細(xì)胞的長效抑制作用[J];離子交換與吸附;2009年02期

6 楊照;周新民;樊代明;韓英;時永全;王敬博;張懿;;生物型人工肝的新種子細(xì)胞來源-誘導(dǎo)性多潛能干細(xì)胞[J];胃腸病學(xué)和肝病學(xué)雜志;2010年03期

7 魯元剛,伍津津,朱堂友,胡浪,賀萍,楊宏珍;復(fù)合殼多糖人工皮膚組織學(xué)特征研究[J];中國臨床康復(fù);2002年04期

8 曹誼林,蔡霞,崔磊,商慶新,劉偉,關(guān)文祥;自體組織工程化全層皮膚缺損修復(fù)實(shí)驗(yàn)研究[J];中華外科雜志;2002年01期

9 王旭,王甲漢,吳軍,趙陽兵,趙雄飛;復(fù)合皮的制作與臨床應(yīng)用[J];中國修復(fù)重建外科雜志;1997年02期

10 黃偉琪;趙耀華;鄧海濤;徐麗紅;沈耀明;袁東亮;;基因轉(zhuǎn)染豬皮在深度燒傷創(chuàng)面治療中的應(yīng)用[J];中華損傷與修復(fù)雜志(電子版);2012年04期

相關(guān)碩士學(xué)位論文 前2條

1 徐冰函;聚乙烯醇水凝膠制備及生物評價[D];吉林大學(xué);2011年

2 崔巍巍;載銀PLGA抗菌敷料的制備及毒性評價[D];吉林大學(xué);2010年

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