本文選題：聚乳酸 + 靜電紡絲　； 參考：《西南交通大學》2012年碩士論文

【摘要】：目前納米材料表面的形態(tài)特征對細胞功能的影響,吸引了越來越多學者的關(guān)注。在再生醫(yī)學和組織工程支架材料研究中的一個基本研究目的是細胞與材料之間的相互作用,其中材料表面的微觀結(jié)構(gòu),尤其是微納米結(jié)構(gòu)對細胞的生物調(diào)控作用是研究的重要內(nèi)容之一。材料的表面形貌和特殊納米性質(zhì)能夠影響細胞的行為,如黏附、增殖、遷移和分化等。 首先,本論文利用靜電紡絲的方法對納米纖維的表面粗糙度進行調(diào)節(jié),通過調(diào)整聚合物溶液的濃度來控制纖維上珠子的形貌,其表面特征用掃描電子顯微鏡(SEM)來表征,利用軟件計算珠子的長寬比,用原子力顯微鏡(AFM)來表征表面粗糙度(△h)。 其次,我們在本實驗室自提取出生10天左右的SD鼠脛骨骨髓原代細胞,定時更換培養(yǎng)基除去造血細胞,經(jīng)過培養(yǎng)、傳代時嚴格控制胰酶的量和消化時間,不斷純化得到骨髓間充質(zhì)干細胞(rat-BMSCs)。 然后,通過在體外與材料共培養(yǎng)骨髓間質(zhì)干細胞,我們研究了纖維表面粗糙度對細胞黏附、鋪展、增殖等細胞行為的影響。研究結(jié)果顯示：靜電紡絲納米纖維上存在的串珠狀結(jié)構(gòu)對細胞的生長不會帶來任何負面影響,反之,珠子在表面堆積,產(chǎn)生高低不同的凸起,即產(chǎn)生不同的納米粗糙度,可以調(diào)節(jié)和促進細胞的行為。因此,通過實驗得出具有合適的粗糙度的靜電紡纖維,在一定程度上更適合作為組織工程支架。 最后,為了進一步研究纖維表面粗糙度對成骨誘導(dǎo)的效應(yīng),探究其在骨代替物的作用,我們將它們進行了體外礦化和體內(nèi)植入實驗。實驗結(jié)果顯示：白鼠皮下植入后,炎癥反應(yīng)并不明顯,具有合適粗糙度的纖維具有比較顯著的體內(nèi)成骨誘導(dǎo)現(xiàn)象,并且在第6周時,纖維能在體內(nèi)部分降解。體內(nèi)實驗進一步說明具有合適的粗糙度的靜電紡纖維具有較大的作為組織工程支架應(yīng)用的潛力。
[Abstract]:At present, the effect of surface morphology on cell function of nanomaterials has attracted more and more attention.One of the basic purposes of research in regenerative medicine and tissue engineering scaffold materials is the interaction between cells and materials, in which the microstructure of the surface of materials,In particular, the biological regulation of microstructures on cells is one of the important research contents.The surface morphology and special nanometer properties of the materials can affect cell behavior, such as adhesion, proliferation, migration and differentiation.Firstly, the surface roughness of nanofibers was adjusted by electrostatic spinning. The morphology of beads on the fibers was controlled by adjusting the concentration of polymer solution, and the surface characteristics were characterized by scanning electron microscopy (SEM).The aspect ratio of beads was calculated by software and the surface roughness was characterized by atomic force microscope (AFM).Secondly, we extracted the primary cells from the bone marrow of SD rats born about 10 days in our laboratory, and periodically changed the culture medium to remove hematopoietic cells. After the culture, we strictly controlled the amount of trypsin and digestion time during passage.The bone marrow mesenchymal stem cells (BMSCs) were purified continuously.Then, by co-culture of bone marrow mesenchymal stem cells in vitro, we studied the effects of fiber surface roughness on cell adhesion, spreading and proliferation.The results show that the beaded structure on the electrospun nanofibers does not have any negative effect on cell growth. Instead, beads accumulate on the surface and produce different bulges, that is, different nano-roughness.Can regulate and promote the behavior of cells.Therefore, the electrospun fibers with suitable roughness were found to be more suitable for tissue engineering scaffold.Finally, in order to further study the effect of fiber surface roughness on osteogenesis and its role in bone substitute, we carried out in vitro mineralization and in vivo implantation experiments.The results showed that the inflammatory reaction was not obvious after subcutaneous implantation of white rats, and the fibers with suitable roughness had significant osteogenic induction in vivo, and at the 6th week, the fibers could be partially degraded in vivo.In vivo experiments further show that electrospun fibers with suitable roughness have great potential as scaffolds for tissue engineering.
【學位授予單位】：西南交通大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.08

【引證文獻】

相關(guān)碩士學位論文 前1條

1 劉霞;RGD接枝氧化海藻酸鈉/N-琥珀酰殼聚糖復(fù)合水凝膠的制備及體外誘導(dǎo)分化研究[D];西南交通大學;2013年

，

本文編號：1761612