本文選題：含銅鈦合金 + 金黃色葡萄球菌��； 參考：《佳木斯大學(xué)》2014年碩士論文

【摘要】：目的：通過體外實(shí)驗(yàn)研究經(jīng)粉末冶金方法制備的含銅鈦合金對大腸桿菌和金黃色葡萄球菌的抗菌性能，為發(fā)展合金型抗菌鈦合金提供理論指導(dǎo)。 方法：經(jīng)粉末冶金方法制備含銅鈦合金，其中銅含量為10wt%，經(jīng)手工混合鈦銅粉末后熱壓燒結(jié)為A組，經(jīng)機(jī)械球磨鈦銅粉末熱壓燒結(jié)為B組，其制備工藝參數(shù)不同分為B1～B9組。機(jī)械球磨鈦銅粉末熱壓燒結(jié)為C組，其銅含量不同分為C1～C4組，醫(yī)用純鈦為對照組。選擇金黃色葡萄球菌和大腸桿菌作為實(shí)驗(yàn)菌株。采用平板菌落計(jì)數(shù)法進(jìn)行評價含銅鈦合金的抗菌性能，并經(jīng)過連續(xù)3次拋光處理，檢測含銅鈦合金抗菌性能的長效性。從B1～B9組中篩選出對金黃色葡萄球菌和大腸桿菌都具有最佳抗菌性能的試件，分別進(jìn)行3h，7h，24h的抗菌率測試。C組進(jìn)行硬度、抗壓強(qiáng)度、耐腐蝕性能和銅離子溶出測試。 結(jié)果:與醫(yī)用純鈦相比，經(jīng)過連續(xù)三次拋光處理，A組對金黃色葡萄球菌和大腸桿菌的抗菌率均大于99%，表明A組具有穩(wěn)定的強(qiáng)抗菌作用。B1，，B5，B6，B8對金黃色葡萄球菌的抗菌率均大于99%，B2-B4，B7，B9對金黃色葡萄球菌的抗菌率均大于90%，而B1-B5，B7，B8對大腸桿菌的抗菌率均大于99%，B6，B9對大腸桿菌的抗菌率均大于90%；其中B1，B5，B8經(jīng)過連續(xù)三次拋光處理后對金黃色葡萄球菌和大腸桿菌的抗菌率均大于99%，表明B1，B5，B8具有穩(wěn)定的強(qiáng)抗菌作用，其中B5的抗菌活性最佳。培養(yǎng)3h，7h，24h后B5對大腸桿菌和金黃色葡萄球菌的抗菌率分別為35.5%和42.8%，99.0%和91.7%，99.9%和100.0%，可見隨著培養(yǎng)時間的延長抗菌率逐漸增加。C1對金黃色葡萄球菌和大腸桿菌的抗菌率均小于90%，表明C1不具有抗菌活性。C2對金黃色葡萄球菌和大腸桿菌的抗菌率均大于90%，表明C2具有抗菌作用。C3和C4對大腸桿菌和金黃色葡萄球菌的抗菌率均大于99%，這表明C3和C4具有強(qiáng)的抗菌作用。銅的添加提高了鈦銅合金的硬度、抗壓強(qiáng)度和耐腐蝕性能，但同時降低了延展性。銅離子釋放濃度隨著銅含量和浸泡時間的增加而增大。 結(jié)論：粉末冶金能制備出整體具有抗菌活性的含銅鈦合金。其銅含量為5wt%能獲得對金黃色葡萄球菌和大腸桿菌穩(wěn)定的抗菌作用，能提高了合金的硬度、抗壓強(qiáng)度和耐腐蝕性能。
[Abstract]:Objective: to study the antibacterial properties of copper-bearing titanium alloy prepared by powder metallurgy against Escherichia coli and Staphylococcus aureus in vitro, and to provide theoretical guidance for the development of alloy type antibacterial titanium alloy.Methods: the copper-bearing titanium alloy was prepared by powder metallurgy. The content of copper was 10 wt. After manual mixing of titanium and copper powder, hot pressing sintering was used as group A and mechanical ball milling of titanium and copper powder as group B. The preparation process parameters were divided into B1~B9 group.The content of copper in mechanical ball milling titanium copper powder was divided into C1~C4 group and medical pure titanium group.Staphylococcus aureus and Escherichia coli were selected as experimental strains.The antibacterial properties of Cu-containing titanium alloys were evaluated by colony counting method, and the long-term antibacterial properties of copper-containing titanium alloys were tested after three successive polishing treatments.Samples with the best antibacterial activity against Staphylococcus aureus and Escherichia coli were selected from the B1~B9 group. The antibacterial rate of group C. the hardness, compressive strength, corrosion resistance and copper ion dissolution were tested for 3 h and 7 h / 24 h respectively.Results: compared with medical pure titanium,The antimicrobial activity of group A against Staphylococcus aureus and Escherichia coli was higher than that of group A after three successive polishing treatments, which indicated that group A had strong antibacterial activity against Staphylococcus aureus.The antimicrobial rate of B1-B5OB7B8 against Escherichia coli was all greater than that of B1-B5OB7B8 against Escherichia coli, and the antimicrobial rate of B1-B5OB8 against Staphylococcus aureus and Escherichia coli was higher than that of B1-B5B8.The results show that B _ 1 B _ 5 B _ 5 B _ 8 has a stable and strong antibacterial activity.Among them, B 5 has the best antibacterial activity.The antimicrobial rates of B5 against Escherichia coli and Staphylococcus aureus were 35.5% and 42.80.99.0% and 91.799.9% and 100.0% respectively after incubation for 3 h and 7 h / 24 h, respectively. It can be seen that the antimicrobial rate of B5 against Staphylococcus aureus and Escherichia coli increased gradually with the extension of culture time. The antimicrobial rate of B5 against Staphylococcus aureus and Escherichia coli was small.The antimicrobial activity of C1 against Staphylococcus aureus and Escherichia coli was higher than 90%, and the antibacterial activity of C2 against Escherichia coli and Staphylococcus aureus was higher than 90%, which indicated that the antibacterial activity of C2 against Escherichia coli and Staphylococcus aureus was greater than 99%.C3 and C4 have strong antibacterial effect.The addition of copper can improve the hardness, compressive strength and corrosion resistance of Ti Cu alloy, but decrease the ductility at the same time.The release concentration of copper ion increases with the increase of copper content and soaking time.Conclusion: copper-containing titanium alloy with antibacterial activity can be prepared by powder metallurgy.Its copper content of 5 wt% can obtain stable antibacterial effect against Staphylococcus aureus and Escherichia coli, and can improve the hardness, compressive strength and corrosion resistance of the alloy.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R783.1

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