發(fā)布時(shí)間：2018-04-15 07:33

  本文選題：降鈣素基因相關(guān)肽 + 神經(jīng)肽Y　； 參考：《中國(guó)組織工程研究》2017年08期

【摘要】：背景:神經(jīng)肽作為存在于神經(jīng)組織的一種內(nèi)源性活性物質(zhì),作用于全身多個(gè)系統(tǒng),調(diào)節(jié)體內(nèi)各系統(tǒng)生理功能。目的:觀察分別植入單純血管束、感覺(jué)神經(jīng)束的組織工程骨修復(fù)兔大段骨缺損對(duì)降鈣素基因相關(guān)肽、神經(jīng)肽Y表達(dá)的影響。方法:新西蘭兔共54只制備股骨大段骨缺損模型,以隨機(jī)抽簽法分為感覺(jué)神經(jīng)束植入組、血管束植入組及對(duì)照組,每組各18只,分別給予單純感覺(jué)神經(jīng)束植入、單純血管束植入及無(wú)血管神經(jīng)束組織工程骨(對(duì)照組)修復(fù)治療,分別于造模后4,8,12周對(duì)修復(fù)骨段行大體、Masson染色觀察,比較修復(fù)骨段內(nèi)降鈣素基因相關(guān)肽和神經(jīng)肽Y表達(dá)水平。結(jié)果與結(jié)論:(1)造模后4,8,12周,感覺(jué)神經(jīng)束植入、血管束植入組降鈣素基因相關(guān)肽和神經(jīng)肽Y mRNA表達(dá)水平均顯著高于對(duì)照組(P0.05);(2)組織工程骨內(nèi)降鈣素基因相關(guān)肽和神經(jīng)肽Y mRNA表達(dá)水平隨時(shí)間增加先增高后下降,其中第8周降鈣素基因相關(guān)肽和神經(jīng)肽Y mRNA表達(dá)水平顯著高于第4,12周(P0.05);且第4周降鈣素基因相關(guān)肽和神經(jīng)肽Y mRNA表達(dá)水平顯著低于其他時(shí)間點(diǎn)(P0.05);(3)組織工程骨內(nèi)降鈣素基因相關(guān)肽主要表達(dá)于新生骨骨質(zhì)邊緣、骨膜及血管外周;神經(jīng)肽Y主要表達(dá)于髓腔及血管外周;(4)結(jié)果說(shuō)明,骨缺損中血管束或感覺(jué)神經(jīng)束植入均可顯著上調(diào)神經(jīng)肽降鈣素基因相關(guān)肽和神經(jīng)肽Y合成水平,加快骨質(zhì)修復(fù)進(jìn)程;但感覺(jué)神經(jīng)束植入組織工程骨可能引起支配區(qū)感覺(jué)功能喪失,故血管束植入更符合正常人體生理功能需求。
[Abstract]:Background: as an endogenous active substance in nerve tissues, neuropeptides act on multiple systems throughout the body and regulate physiological functions of various systems in vivo.Aim: to investigate the effects of tissue engineering bone grafted with vascular bundle and sensory nerve bundle on the expression of calcitonin gene-related peptide and neuropeptide Y in rabbits with large bone defects.Methods: 54 New Zealand rabbits were randomly divided into three groups: sensory nerve bundle implantation group, vascular bundle implantation group and control group. 18 rabbits in each group were given simple sensory nerve bundle implantation.Simple vascular bundle implantation and tissue engineering bone repair (control group) without vascular and nerve bundles were observed by Masson staining at the 12th week after modeling. The expression levels of calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) in the repaired bone segment were compared.Results and conclusion the sensory nerve bundle was implanted in the rat model at 8 ~ 12 weeks after the establishment of the model.The expression of calcitonin gene-related peptide and neuropeptide Y mRNA in vascular bundle implantation group was significantly higher than that in the control group (P 0.05). The expression level of calcitonin gene-related peptide and neuropeptide Y mRNA in tissue engineering bone was increased firstly and then decreased with time.The expression level of calcitonin gene-related peptide and neuropeptide Y mRNA was significantly higher in the 8th week than that in the 4th week, and the expression level of calcitonin gene-related peptide and neuropeptide Y mRNA in the 4th week was significantly lower than that in the other time points (P0.05P0.05).Calcitonin gene-related peptide is mainly expressed at the margin of bone.The results showed that vascular bundle or sensory nerve bundle implantation in bone defect could significantly upregulate the synthesis of neuropeptide calcitonin gene-related peptide and neuropeptide Y.However, sensory nerve bundle implantation may cause loss of sensory function in the innervation area, so vascular bundle implantation is more suitable for physiological function of normal human body.
【作者單位】： 廣西壯族自治區(qū)人民醫(yī)院骨科;解放軍第四軍醫(yī)大學(xué)西京醫(yī)院骨科;南方醫(yī)科大學(xué)第一臨床醫(yī)學(xué)院;廣東醫(yī)科大學(xué)附屬醫(yī)院骨科;廣州醫(yī)科大學(xué)附屬第三醫(yī)院骨科;深圳市第九醫(yī)院骨科;海南省人民醫(yī)院創(chuàng)傷骨科;
【基金】：國(guó)家自然科學(xué)基金(30600643)~~
【分類號(hào)】：R318.08;R683

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