本文選題：三維打印　切入點(diǎn)：蠶絲蛋白　出處：《浙江大學(xué)》2015年碩士論文

【摘要】：絲素蛋白作為一種常用的組織工程支架材料,其降解性能一直不甚清楚,本文從快速降解和酶降解的角度探究和分析了絲素蛋白的降解行為,以及根據(jù)降解性能分析結(jié)果設(shè)計和改良了絲素蛋白材,并用于軟組織的修復(fù)。發(fā)現(xiàn)經(jīng)過脫膠及后期成型加工處理的絲素蛋白支架的降解速率偏慢,在60天的高溫降解以及42天的酶溶液水解后仍有大部分絲素蛋白支架穩(wěn)定存在。 皮膚修復(fù)不理想一方面是因為真皮層中缺乏血管的形成,缺少為受傷的皮膚組織提供氧氣和營養(yǎng)的渠道,另一方面是由于表皮的再生困難導(dǎo)致傷口難以閉合,需要一個良好的真皮環(huán)境促進(jìn)表皮的遷移及閉合。因此,制備出在生理上能夠滿足全層皮膚缺損再生要求的活性支架是一項具有挑戰(zhàn)性的工作。為了克服這一困難,在這項研究中,我們開發(fā)了一個三維打印水凝膠-絲素蛋白支架(3DG-SF),利用修飾在絲素蛋白上的磺酸根(S03)復(fù)合堿性成纖維細(xì)胞生長因子(FGF-2)形成一個三維的生物活性支架(3DG-SF-SO3-FGF)。利用體外細(xì)胞培養(yǎng)和體內(nèi)全層皮膚缺損的動物模型研究了3DG-SF-SO3-FGF活性支架對大鼠全層皮膚缺損再生的治療效果。在植入支架14和28天后的全層皮膚缺陷模型的大鼠背部,組織學(xué)結(jié)果顯示與凡士林紗布對照組相比,結(jié)合FGF-2的三維打印支架能顯著增強(qiáng)皮膚組織的再生。進(jìn)一步實驗表明,結(jié)合FGF-2的三維打印支架可能在刺激真皮層中血管形成上具有一定的功能。這可能是FGF-2通過激活真皮血管組織的萌芽提高組織再生。這些發(fā)現(xiàn)表明,結(jié)合FGF-2的3DG-SF-SO3支架是一種有益皮膚組織工程的支架,在促進(jìn)皮膚的再生研究上具有一定的應(yīng)用價值。
[Abstract]:As a commonly used scaffold material for tissue engineering, the degradation performance of silk fibroin is not clear. In this paper, the degradation behavior of silk fibroin was studied and analyzed from the point of view of rapid degradation and enzymatic degradation.The silk fibroin material was designed and improved according to the results of degradation analysis, and was used for soft tissue repair.It was found that the degradation rate of silk fibroin scaffolds treated by degumming and later processing was slow. After 60 days of high temperature degradation and 42 days of hydrolysis of enzyme solution, most of silk fibroin scaffolds remained stable.Skin repair is not ideal because, on the one hand, there is a lack of blood vessel formation in the dermis, a lack of channels to provide oxygen and nutrition to the injured skin tissue, and on the other hand, the wound is difficult to close due to difficulties in the regeneration of the epidermis.A good dermal environment is needed to facilitate the migration and closure of the epidermis.Therefore, it is a challenging task to prepare bioactive scaffolds that can meet the requirements of full-thickness skin defect regeneration.In order to overcome this difficulty, in this study,A three-dimensional printing hydrogel-fibroin scaffold (3DG-SFG) was developed to form a three-dimensional bioactive scaffold (3DG-SF-SO3-FGFG) using sulfonic acid group S03( modified on fibroin protein) combined with basic fibroblast growth factor (FGF-2).The effect of 3DG-SF-SO3-FGF active scaffold on the regeneration of rat full-thickness skin defect was studied by using the animal model of in vitro cell culture and in vivo full-thickness skin defect.The histological results of the full-thickness skin defect model rats after 14 and 28 days of stenting showed that the three-dimensional printing scaffold combined with FGF-2 could significantly enhance the regeneration of skin tissue compared with the control group of vaseline gauze.Further experiments show that the 3D printing scaffold combined with FGF-2 may play a role in stimulating vascular formation in the dermis.It is possible that FGF-2 enhances tissue regeneration by activating the bud of dermal vascular tissue.These findings suggest that the 3DG-SF-SO3 scaffold combined with FGF-2 is a useful scaffold for skin tissue engineering and has certain application value in promoting skin regeneration.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R318.08

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本文編號：1729612