本文選題：骨髓　切入點：間質干細胞　出處：《中國組織工程研究》2017年13期

【摘要】：背景:目前有一些關于骨髓間充質干細胞復合異體骨、人工合成骨替代材料促進骨缺損修復的研究,但尚未見骨髓間充質干細胞復合Bio-oss用于兔顱骨缺損修復的報道。目的:觀察骨髓間充質干細胞復合Bio-oss修復兔顱骨缺損的效果。方法:分離培養(yǎng)雄性兔骨髓間充質干細胞,將其作為種子細胞,在雌兔顱骨采用外直徑6 mm的環(huán)形骨鉆制造全層骨缺損3個。將96只雌性兔隨機等分為4組,復合物組植入Bio-oss復合骨髓間充質干細胞,Bio-oss組單純植入Bio-oss,骨髓間充質干細胞組單純植入骨髓間充質干細胞,空白組不進行植入修復。每個組表面均覆蓋引導組織再生膜。結果與結論:復合物組成骨效果比其他3組更佳,Bio-oss組成骨效果比骨髓間充質干細胞組和空白組更好,骨髓間充質干細胞組與空白組成骨效果接近。說明以骨髓間充質干細胞作為種子細胞,Bio-oss作為支架材料復合植入兔顱骨缺損區(qū)有明顯的促進成骨作用,為臨床治療骨缺損提供新思路。
[Abstract]:Background: there have been some studies on bone marrow mesenchymal stem cells (BMSCs) combined with allogeneic bone, synthetic bone substitute materials to promote bone defect repair, but no reports of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-oss for repairing rabbit skull defects have been reported.Objective: to observe the effect of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-oss in repairing rabbit skull defect.Methods: male rabbit bone marrow mesenchymal stem cells were isolated and cultured as seed cells.96 female rabbits were randomly divided into 4 groups. The composite group was implanted with Bio-oss combined with bone marrow mesenchymal stem cells (BMSCs), the bone marrow mesenchymal stem cells (BMSCs) group was implanted with BMSCs only, and the blank group was not implanted with BMSCs.The surface of each group was covered with guided tissue regeneration membrane.Results and conclusion: the effect of composite bone composition was better than that of the other three groups. The effect of Bio-oss was better than that of bone marrow mesenchymal stem cells group and blank group. The effect of bone marrow mesenchymal stem cell group was similar to that of blank bone.It is concluded that the composite implantation of bone marrow mesenchymal stem cells (BMSCs) as seed cells and Bio-oss as scaffold material can obviously promote osteogenesis in rabbit skull defect and provide a new idea for clinical treatment of bone defect.
【作者單位】： 西南醫(yī)科大學口腔醫(yī)學院;解放軍成都軍區(qū)機關醫(yī)院口腔科;西南醫(yī)科大學附屬口腔醫(yī)院口腔頜面外科;
【基金】：解放軍成都軍區(qū)“十二五”面上項目(C14050)~~
【分類號】：R318.08;R68
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本文編號：1704454