本文選題：小腸黏膜下層　切入點(diǎn)：脫細(xì)胞處理　出處：《中國生物工程雜志》2015年06期

【摘要】：目的:采用三種不同的脫細(xì)胞方法制備小腸黏膜下層(small intestinal submucosa,SIS)支架,研究其生物相容性、免疫原性以及構(gòu)建真皮替代物等方面,為組織工程化皮膚的細(xì)胞載體支架提供優(yōu)選方案。方法:將新鮮豬小腸分別采用單純機(jī)械法、機(jī)械-化學(xué)法、機(jī)械-酶消化法制備三種不同的SIS,分別作為A、B、C組;通過復(fù)合成纖維細(xì)胞構(gòu)建真皮替代物,利用HE染色觀察支架組織學(xué)形態(tài)以及細(xì)胞粘附情況、MTT法檢測細(xì)胞增殖情況,同時(shí)觀察支架移植SD大鼠皮下1、2、4周后炎癥反應(yīng)及血管化程度。結(jié)果:組織學(xué)觀察A組有細(xì)胞殘留,B、C組未見細(xì)胞殘留。MTT結(jié)果顯示細(xì)胞在支架上生長旺盛增殖能力強(qiáng),其中A組優(yōu)于B、C組;皮下移植后的HE結(jié)果表明B組引起炎癥反應(yīng)較A、C組弱,而C組血管化程度較A、B組更明顯。結(jié)論:機(jī)械-化學(xué)法以及機(jī)械-酶消化法制備的SIS具有良好的生物相容性以及免疫原性,可作為構(gòu)建組織工程化皮膚細(xì)胞載體的選擇。
[Abstract]:Objective: to study the biocompatibility, immunogenicity and dermis substitute of small intestinal submucosae SISs scaffolds prepared by three different acellular methods. Methods: three kinds of SISs were prepared by simple mechanical method, mechanical-chemical method and mechanical-enzyme digestion method, respectively. Dermal substitutes were constructed by composite fibroblasts. Histological morphology of scaffolds and cell adhesion were observed by HE staining. MTT assay was used to detect cell proliferation. At the same time, the inflammatory reaction and the degree of vascularization in SD rats were observed after 4 weeks of subcutaneous transplantation. Results: the results of histological observation showed that there were no cell residues in group A, and MTT showed that the cells could proliferate strongly on the scaffold. The results of HE after subcutaneous transplantation showed that the inflammatory response in group B was weaker than that in group A. Conclusion: SIS prepared by mechanical-chemical method and mechano-enzyme digestion has good biocompatibility and immunogenicity, and can be used as a carrier for constructing tissue engineered skin cells.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院整形外科;
【基金】：廣東省自然科學(xué)基金(S2011010003864)資助項(xiàng)目
【分類號(hào)】：R318.08

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 王佃亮;;組織工程的誕生與發(fā)展——組織工程連載之一[J];中國生物工程雜志;2014年05期

相關(guān)碩士學(xué)位論文 前1條

1 王宇，

本文編號(hào)：1670672