本文選題：肝素　切入點：白蛋白　出處：《西南交通大學(xué)》2012年碩士論文

【摘要】：本論文通過層層靜電自組裝法在超薄的高純鈦表面交替組裝肝素和白蛋白分子層。高純鈦通過堿活化后表面呈負(fù)電性,多聚賴氨酸在PBS溶液中呈正電性,通過靜電作用可將多聚賴氨酸吸附于堿活化的鈦表面,然后將其交替浸泡于帶負(fù)電的肝素溶液和帶正電的白蛋白溶液中形成多層自組裝膜。最后在不同彎曲半徑下使之受力彎曲,通過傅里葉紅外漫反射(FTIR)檢測表面基團(tuán)的變化,通過接觸角檢測對組裝過程中表面親水性以及表面能的變化進(jìn)行表征,通過激光共聚焦顯微鏡和掃描電子顯微鏡觀察材料表面形貌的變化,以了解外力作用對組裝生物分子后的材料表面理化性能和血液相容性影響。 (1)傅里葉紅外漫反射(FTIR)結(jié)果顯示肝素和白蛋白成功的組裝到鈦表面。水接觸角結(jié)果進(jìn)一步表明各步處理成功。隨著組裝層數(shù)的增加,水接觸角的值呈上升的趨勢。 (2)激光共聚焦顯微鏡結(jié)果表明,鈦表面組裝的生物分子層在基底材料彎曲變形的過程中,有裂紋出現(xiàn)。 (3)掃描電鏡結(jié)果表明,在彎曲直徑為Φ5mm,即材料延伸率為1.6%的時候,出現(xiàn)明顯裂紋,且隨著膜厚的增加,裂紋數(shù)量增多,且有擴(kuò)展的趨勢。 (4)血小板黏附實驗表明,彎曲變形后,表面血小板黏附數(shù)量有一定增加,其中彎曲直徑為φ15mm和φ10mm時,血小板數(shù)量最多。變形后,在不同彎曲直徑條件下,血小板的聚集程度增加。無論變形前后,對于組裝7層、12層、17層的樣品,17層血小板黏附數(shù)量最少。 綜上所述,構(gòu)建了生物修飾層的鈦表面在受到外力作用而彎曲的時候,其表面性質(zhì)發(fā)生改變。
[Abstract]:In this paper, the layer by layer electrostatic self-assembly method was used to assemble heparin and albumin molecular layers alternately on the surface of ultra-thin high-purity titanium. The surface of high-purity titanium was negatively charged after alkali activation, and poly-lysine was positively electrically present in PBS solution. Polysine can be adsorbed on the surface of alkaline activated titanium by electrostatic action. Then they were immersed alternately in a negatively charged heparin solution and a positively charged albumin solution to form a multilayer self-assembled film. Finally, the surface groups were measured by Fourier transform infrared diffuse reflectance (FTIR). The changes of surface hydrophilicity and surface energy during assembly were characterized by contact angle measurement, and the changes of surface morphology were observed by laser confocal microscope and scanning electron microscope. In order to understand the effect of external force on the surface physical and chemical properties and blood compatibility of the biomolecules assembled. The FTIR results showed that heparin and albumin were successfully assembled on the titanium surface. The water contact angle showed that the water contact angle increased with the increase of the number of the assembled layers. (2) the results of laser confocal microscopy show that there are cracks in the biomolecular layer of titanium surface during the bending deformation of the substrate material. The SEM results show that obvious cracks appear when the bending diameter is 桅 5mm, that is, the elongation of the material is 1.6%, and with the increase of film thickness, the number of cracks increases and tends to propagate. 4) Platelet adhesion test showed that the number of platelet adhesion on the surface increased after bending deformation, especially when the bending diameter was 蠁 15mm and 蠁 10mm, the number of platelets was the most when the bending diameter was 蠁 15mm and 蠁 10mm. The platelet aggregation was increased. No matter before and after deformation, the number of platelet adhesion in 17 layers was the least for the sample of 7 layers and 12 layers and 17 layers. In conclusion, the surface properties of the modified titanium surface changed when it was bent by external force.
【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

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