發(fā)布時(shí)間：2018-03-24 15:37

  本文選題：生物3D打印　切入點(diǎn)：多層皮膚模型　出處：《杭州電子科技大學(xué)》2017年碩士論文

【摘要】：由燒傷、創(chuàng)傷及不愈合潰瘍等導(dǎo)致的大面積的皮膚缺損創(chuàng)面如不及時(shí)治療,會(huì)造成患者體液大量丟失、繼發(fā)嚴(yán)重感染,嚴(yán)重威脅患者生命。常用的治療措施包括自體皮膚移植、異體/異種皮膚移植、細(xì)胞活性因子、細(xì)胞治療、真皮替代物等,但傳統(tǒng)治療方法在皮膚修復(fù)時(shí)間、治療成本、治療效果等方面依然存在不足。生物3D打印基于快速成型分層制造的原理,可將細(xì)胞及基質(zhì)材料等物質(zhì)準(zhǔn)確輸送與定位,構(gòu)建具有仿生三維結(jié)構(gòu)及功能的類組織前體,為構(gòu)建復(fù)雜仿生結(jié)構(gòu)的組織模型研究提供了新的技術(shù)可能。對比于傳統(tǒng)皮膚組織工程技術(shù),生物三維打印技術(shù)具有細(xì)胞定位精確,逐層打印高效的特點(diǎn),可使制造周期大幅縮短,提高效率。本研究基于生物3D打印設(shè)備,自手術(shù)廢棄皮片中提取真皮成纖維細(xì)胞和表皮角質(zhì)形成細(xì)胞、制備細(xì)胞/生物相容性材料復(fù)合凝膠,基于皮膚分層結(jié)構(gòu)進(jìn)行打印,構(gòu)建與人體皮膚結(jié)構(gòu)類似的組織。研究首先對組織工程皮膚研究現(xiàn)狀、生物3D打印技術(shù)背景和研究進(jìn)展做了簡要綜述,總結(jié)現(xiàn)有組織工程皮膚技術(shù)局限,對基于生物3D打印技術(shù)構(gòu)建多層皮膚組織可行性做了探討。其次簡單的介紹團(tuán)隊(duì)研發(fā)的3D Bio-Architect○R-WS組成系統(tǒng)及皮膚打印基質(zhì)材料的選擇,之后對來自人體皮膚的角質(zhì)形成細(xì)胞和成纖維細(xì)胞的提取和培養(yǎng)過程進(jìn)行闡述,然后依據(jù)人體皮膚真實(shí)的結(jié)構(gòu),設(shè)計(jì)人體皮膚三維結(jié)構(gòu)模型,將人體來源的成纖維細(xì)胞/膠原/明膠/海藻酸鈉作為真皮層、角質(zhì)形成細(xì)胞/Ⅰ型膠原作為表皮層,通過生物3D打印設(shè)備嚴(yán)格按照人體皮膚真實(shí)結(jié)構(gòu)精準(zhǔn)裝配組成多層皮膚組織模型。利用MTT比色法檢測打印過程對支架中成纖維細(xì)胞存活率的影響,其次用熒光染色法和HE染色對人體皮膚分層結(jié)構(gòu)作了研究。研究結(jié)果證明,基于生物3D打印技術(shù),能夠快速有效的在體外構(gòu)建高仿真的多層皮膚組織模型。本研究為快速制備具有多層仿生結(jié)構(gòu)的人工皮膚用于皮膚修復(fù)、化妝品安全性測試和藥物滲透性研究提供了新的方法。
[Abstract]:Large areas of skin defects caused by burns, wounds and nonunion ulcers, if not treated in time, will result in massive loss of body fluids, secondary severe infection and serious threat to the life of the patients. Common treatment measures include autogenous skin transplantation. Allograft / xenogeneic skin transplantation, cytokines, cell therapy, dermal substitutes, etc. Based on the principle of rapid prototyping and layered manufacturing, biological 3D printing can accurately transport and locate materials such as cells and matrix materials, and construct biomimetic tissue precursors with three-dimensional structure and function. Compared with the traditional skin tissue engineering technology, the three dimensional biological printing technology has the characteristics of accurate location of cells and high efficiency of printing layer by layer, compared with the traditional skin tissue engineering technology, which provides a new technique for the study of tissue models of complex bionic structures. In this study, dermal fibroblasts and epidermal keratinocytes were extracted from abandoned skin grafts to prepare cell / biocompatibility composite gel based on biological 3D printing equipment. Based on skin layering structure, tissue similar to human skin structure was constructed. First of all, the present situation of tissue engineering skin, the background and research progress of biological 3D printing technology were briefly reviewed. This paper summarizes the limitations of existing tissue engineering skin technology, and discusses the feasibility of constructing multilayer skin tissue based on biological 3D printing technology. Secondly, the paper briefly introduces the 3D Bio-Architect0R-WS composition system developed by the team and the selection of skin printing matrix materials. After that, the extraction and culture process of keratinocytes and fibroblasts from human skin are described, and then the three-dimensional structure model of human skin is designed according to the real structure of human skin. Human fibroblasts / collagen / gelatin / sodium alginate were used as dermis and keratinocytes / collagen 鈪，

本文編號：1658933