本文選題：四氧化三鐵　切入點(diǎn)：納米復(fù)合物　出處：《中國(guó)組織工程研究》2016年38期

【摘要】：背景:將磁性納米顆粒作為骨組織工程領(lǐng)域新興材料并植入生物體時(shí),其毒性機(jī)制基礎(chǔ)研究及安全性評(píng)價(jià)就顯得格外重要。目的:探索磁性四氧化三鐵納米顆粒作用于前成骨細(xì)胞的生物相容性。方法:將含有0,200,400,800 mg/L磁性四氧化三鐵納米顆粒的細(xì)胞培養(yǎng)液作用于小鼠前成骨細(xì)胞24 h后,采用堿性磷酸酶活性測(cè)試試劑盒、骨鈣素酶聯(lián)免疫試劑盒、CCK-8試劑盒、倒置顯微鏡、細(xì)胞骨架熒光染色法及實(shí)時(shí)熒光定量PCR,觀察處理后小鼠前成骨細(xì)胞的堿性磷酸酶活性/總蛋白比值、骨鈣素濃度、細(xì)胞增殖率、細(xì)胞形態(tài)和骨架改變、細(xì)胞凋亡以及自噬相關(guān)半胱氨酸蛋白酶3、LC3A、LC3B基因m RNA表達(dá)情況。結(jié)果與結(jié)論:(1)磁性四氧化三鐵納米顆粒作用小鼠前成骨細(xì)胞24 h后,200 mg/L組與對(duì)照組(0 mg/L)相比,各項(xiàng)指標(biāo)差異無(wú)顯著性意義;(2)作用24 h后,400,800 mg/L組細(xì)胞內(nèi)堿性磷酸酶活性/總蛋白比值、骨鈣素濃度上升,細(xì)胞增殖率明顯下降,細(xì)胞形態(tài)及骨架結(jié)構(gòu)改變明顯,LC3B基因轉(zhuǎn)錄水平升高,而半胱氨酸蛋白酶3、LC3A基因轉(zhuǎn)錄水平與對(duì)照組相比差異無(wú)顯著性意義;(3)結(jié)果提示,高質(zhì)量濃度的磁性四氧化三鐵納米顆粒作用于小鼠前成骨細(xì)胞24 h后,雖具有一定的促成骨作用,但同時(shí)可造成細(xì)胞毒性損傷,促進(jìn)細(xì)胞自噬相關(guān)基因LC3B表達(dá)上調(diào),影響細(xì)胞形態(tài)、骨架結(jié)構(gòu)及細(xì)胞增殖率。
[Abstract]:Background: magnetic nanoparticles are used as emerging materials in bone tissue engineering and implanted into organisms. It is particularly important to study the mechanism of toxicity and evaluate its safety. Objective: to explore the biocompatibility of magnetic ferric oxide nanoparticles acting on preosteoblasts. Methods: 0200400800 mg/L magnetic ferrite tetroxide was added to the preosteoblasts. The cell culture medium of rice granules was applied to mouse preosteoblasts for 24 h. Alkaline phosphatase activity test kit, osteocalcin enzyme linked immunoassay kit and CCK-8 kit were used. The ratio of alkaline phosphatase activity to total protein, osteocalcin concentration, cell proliferation rate, morphology and cytoskeleton of preosteoblast were observed by fluorescence staining of cytoskeleton and real-time fluorescence quantitative PCR. Cell apoptosis and m RNA expression of autophagy associated cysteine protease 3 (LC3A) LC3B gene were compared with those of control group (0 mg / L) after treatment with magnetic ferric oxide nanoparticles for 24 h. The ratio of alkaline phosphatase activity to total protein, osteocalcin concentration and proliferation rate of the cells in the 400 800 mg/L group were significantly decreased after 24 h treatment. The changes of cell morphology and cytoskeleton structure showed that the transcriptional level of LC3B gene increased, but the transcription level of cysteine protease 3 + LC3A gene was not significantly different from that of the control group. After high concentration of magnetic ferric oxide nanoparticles acted on mouse preosteoblast for 24 h, although it promoted bone formation, it also caused cytotoxic injury and up-regulated the expression of autophagy related gene LC3B. It affects cell morphology, cytoskeleton structure and cell proliferation rate.
【作者單位】： 中山大學(xué)光華口腔醫(yī)學(xué)院·附屬口腔醫(yī)院口腔頜面外科廣東省口腔醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(81070818)~~
【分類號(hào)】：R318.08

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本文編號(hào)：1655217