本文選題：陣列式化學(xué)傳感器　切入點(diǎn)：點(diǎn)樣儀　出處：《重慶大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：隨著化學(xué)傳感器技術(shù)的發(fā)展,對于簡單、便捷、快速和應(yīng)用廣泛的檢測分析方法的迫切需求,陣列式化學(xué)傳感器應(yīng)運(yùn)而生,并且在科學(xué)研究和實(shí)際生活中得到了廣泛應(yīng)用,尤其體現(xiàn)在分析檢測復(fù)雜化合物方面。由于陣列式化學(xué)傳感器具有靈敏度高、響應(yīng)速度快和可集成度高等優(yōu)點(diǎn),被廣泛應(yīng)用食品、糖類、有毒氣體和疾病等領(lǐng)域的檢測。大量研究實(shí)驗(yàn)表明卟啉及衍生物作為傳感元件,具有很高的靈敏度和化學(xué)穩(wěn)定性,在對目標(biāo)物的特異性識別與鑒定方面體現(xiàn)出特殊功能,因此,如何構(gòu)建可靠的可視化卟啉化學(xué)傳感陣列,對多學(xué)科交叉領(lǐng)域的研究具有很重要的意義。生成像如可視化卟啉化學(xué)傳感陣列的試劑多具有腐蝕性、易揮發(fā)性、混合性的特點(diǎn)。陣列中每個(gè)樣點(diǎn)的試劑成分不同,為了保持每個(gè)樣點(diǎn)直徑相近,制備過程中存在不同試劑擴(kuò)散速度不同的問題。當(dāng)前市面上及專利報(bào)道中沒有專門制備這種特殊陣列的點(diǎn)樣裝置。在功能方面唯一比較接近的是生物芯片點(diǎn)樣儀,但其點(diǎn)樣對象主要是核酸、蛋白質(zhì)、抗體和組織,因此生物芯片點(diǎn)樣儀不可直接制備這種陣列式化學(xué)傳感器。有鑒于此,本課題旨在提供一套可以自動(dòng)制備陣列式化學(xué)傳感器的點(diǎn)樣儀。該儀器可以自動(dòng)吸取高腐蝕性試劑,自動(dòng)完成點(diǎn)樣,并且所制化學(xué)傳感器滿足試驗(yàn)要求。本文的主要工作有一下幾個(gè)方面:一、根據(jù)現(xiàn)有接觸式生物芯片點(diǎn)樣儀的工作原理,設(shè)計(jì)陣列式化學(xué)傳感器點(diǎn)樣儀的整體結(jié)構(gòu)。該結(jié)構(gòu)主要滿足以下幾個(gè)方面的要求:1、實(shí)現(xiàn)X、Y軸兩個(gè)方向的自由運(yùn)動(dòng);2、點(diǎn)樣頭可以加裝36根半徑不同的點(diǎn)樣針;3、可以實(shí)現(xiàn)36根點(diǎn)樣針同時(shí)加樣;4、可以連續(xù)制備多張化學(xué)傳感器;5、試劑槽可以盛裝高腐蝕性化學(xué)試劑。二、根據(jù)點(diǎn)樣儀的整體結(jié)構(gòu)及點(diǎn)樣儀應(yīng)具有的功能,設(shè)計(jì)了以PIC16F877A單片機(jī)為控制核心的硬件系統(tǒng),包括矩陣鍵盤模塊、液晶顯示模塊、電源模塊、定位檢測模塊、步進(jìn)電機(jī)驅(qū)動(dòng)電路模塊。根據(jù)各模塊的功能,在MPLAB中完成整個(gè)控制系統(tǒng)的軟件編寫。三、不同試劑在相同基底上的擴(kuò)散速度不同,因此,在點(diǎn)樣過程中存在不同液體在相同時(shí)間內(nèi)無法擴(kuò)散相同距離的問題。本文根據(jù)毛細(xì)管流體模型、Washburn方程、Laplace方程等,建立了液體在基底上的擴(kuò)散模型,保證不同液體在相同時(shí)間內(nèi)擴(kuò)散相同的距離。四、測試系統(tǒng)各個(gè)模塊,并將各功能模塊組裝,對樣機(jī)進(jìn)行調(diào)試。五、通過對比點(diǎn)樣儀制備的傳感器、手工制備的傳感器分別與VOCs反應(yīng)后兩種陣列差譜圖中各敏感點(diǎn)的歐式距離和樣本相對標(biāo)準(zhǔn)偏差,驗(yàn)證點(diǎn)樣儀制備傳感器比手工制備傳感器更穩(wěn)定,與VOCs響應(yīng)更強(qiáng)烈。
[Abstract]:With the development of chemical sensor technology and the urgent need for simple, convenient, fast and widely used detection and analysis methods, the array chemical sensor has emerged as the times require, and has been widely used in scientific research and practical life. Especially in the analysis and detection of complex compounds. Because of its high sensitivity, high response speed and high integration, the array chemical sensor has been widely used in food, sugar, A large number of experiments show that porphyrin and its derivatives as sensing elements have high sensitivity and chemical stability, and exhibit special functions in the specific identification and identification of the target. How to construct a reliable visual porphyrin chemical sensing array is of great significance to the research of multi-disciplinary cross field. The reagents like visual porphyrin chemical sensing array are corrosive and volatile. Hybrid characteristics. The composition of each sample in the array is different, in order to keep the diameter of each sample close, There is a problem of different reagents diffusing at different speeds in the preparation process. At present, there is no spot device specially prepared for this special array in the market and in patent reports. The only thing that is relatively close in function is the biochip sampler. But its sample is mainly nucleic acid, protein, antibody and tissue, so the biochip spotting instrument can not directly produce this kind of array chemical sensor. The purpose of this paper is to provide a set of sampling instruments that can automatically prepare array chemical sensors. The instrument can automatically absorb highly corrosive reagents and finish the sampling automatically. The main work of this paper is as follows: first, according to the working principle of the existing contact biochip sampler, The overall structure of the array chemical sensor spotting instrument is designed. The structure mainly meets the following requirements: 1: 1, realizes the free movement of XY axis in both directions, and the spot head can be fitted with 36 sampling needles with different radii. It is realized that 36 sample needles can be added to sample 4 simultaneously, and multiple chemical sensors can be prepared continuously. The reagent tank can be filled with highly corrosive chemical reagents. According to the whole structure of the sampling instrument and the function of the sampling instrument, a hardware system based on PIC16F877A microcontroller is designed, which includes matrix keyboard module, liquid crystal display module, power supply module, location detection module, etc. Stepper motor drive circuit module. According to the function of each module, complete the whole control system software in MPLAB. Third, different reagents on the same substrate diffusion speed is different, therefore, In this paper, the diffusion model of liquid on the substrate is established according to the Washburn equation and Laplace equation of capillary fluid. Ensure that different liquids diffused at the same distance within the same time. Fourthly, each module of the test system is assembled, and the prototype is debugged. The Euclidean distance and the sample relative standard deviation of each sensitive point in the two kinds of array differential spectrum diagram after the reaction of the hand-made sensor with VOCs, respectively. It is verified that the sensor prepared by the spot detector is more stable than that of the sensor prepared by hand, and the response of the sensor is stronger than that of the VOCs sensor.
【學(xué)位授予單位】：重慶大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TP212.2;R318

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 霍丹群;張國平;侯長軍;董家樂;張玉嬋;劉振;羅小剛;法煥寶;張宿義;;基于新型可視化學(xué)傳感陣列的氨基酸快速識別[J];分析化學(xué);2010年08期

2 高鳳強(qiáng);郭一晶;于杰;;基于單片機(jī)和THB6128的上粉機(jī)電控系統(tǒng)研究[J];科技和產(chǎn)業(yè);2013年11期

3 閆獻(xiàn)勇,周強(qiáng),宗光華,畢樹生;點(diǎn)樣機(jī)器人的發(fā)展現(xiàn)狀[J];機(jī)器人;2002年06期

4 賈振中;宋立濱;楊東超;陳懇;;接觸式點(diǎn)樣機(jī)器人及相關(guān)技術(shù)研究進(jìn)展[J];機(jī)器人;2007年02期

5 羅春泉;;工業(yè)嵌入式多協(xié)議轉(zhuǎn)換器的研究與應(yīng)用[J];信息技術(shù);2014年03期

6 陳惠明;;圖像歐氏距離在人臉識別中的應(yīng)用研究[J];計(jì)算機(jī)工程與設(shè)計(jì);2008年14期

相關(guān)博士學(xué)位論文 前1條

1 董家樂;基于可視陣列傳感對細(xì)胞代謝過程的研究[D];重慶大學(xué);2011年

，

本文編號：1650287