本文選題：組織工程　切入點(diǎn)：3D生物打印技術(shù)　出處：《華東理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：組織工程具有免疫反應(yīng)小、可以更好的重建原組織形態(tài)、結(jié)構(gòu)和功能等優(yōu)勢。應(yīng)用骨軟骨組織工程治療關(guān)節(jié)損傷疾病已經(jīng)成為一種有效途徑。組織工程的核心是建立由細(xì)胞和支架材料構(gòu)成的三維空間復(fù)合體。三維結(jié)構(gòu)的支架為細(xì)胞提供了營養(yǎng)傳遞和生長代謝的場所,也是形成新的具有形態(tài)和功能的組織、器官的基礎(chǔ)。然而,目前尚無完備的支架設(shè)計(jì)理論用于指導(dǎo)支架設(shè)計(jì),同時(shí)支架體系缺乏縱向?qū)蛹?jí)功能梯度變化,這導(dǎo)致支架無法滿足多種環(huán)境需求。針對(duì)上述支架設(shè)計(jì)缺陷,本文主要目標(biāo)為:(1)建立支架設(shè)計(jì)理論,為支架設(shè)計(jì)體系的研究提供科學(xué)的研究方案;(2)設(shè)計(jì)一種新型雙相梯度三維多孔支架,以滿足支架在修復(fù)損傷過程中的多種需求。為實(shí)現(xiàn)上述目標(biāo),本文的主要工作為:(1)將FCBPSS(Function-Context-Behavior-Principle-State-Structure)理論應(yīng)用于組織工程支架設(shè)計(jì)領(lǐng)域,針對(duì)組織修復(fù)系統(tǒng),提出支架概念設(shè)計(jì)理論;(2)探索合理支架制備材料組分及配方;(3)設(shè)計(jì)支架梯度結(jié)構(gòu),并驗(yàn)證其結(jié)構(gòu)強(qiáng)度;(4)采用3D生物打印技術(shù)制備雙相梯度支架,進(jìn)行支架性能檢測。通過以上工作,本文得到的主要結(jié)論為:(1)FCBPSS理論可科學(xué)有效的指導(dǎo)骨軟骨修復(fù)系統(tǒng)中的支架設(shè)計(jì),為支架設(shè)計(jì)制備提供科學(xué)的理論支撐;(2)由明膠/透明質(zhì)酸(GelMA/HA)所組成的水凝膠體系作為支架軟骨相,聚乳酸-羥基乙酸/聚乙二醇(PLGA/PEG)復(fù)合體系作為支架骨相,可利于3D打印支架制備,同時(shí)適宜細(xì)胞生長增殖分化;(3)本文中所建立梯度結(jié)構(gòu)的支架設(shè)計(jì)方案滿足人體力學(xué)環(huán)境強(qiáng)度需求。本文的主要貢獻(xiàn)在于:(1)在支架設(shè)計(jì)理論方面,將FCBPSS理論作為支架設(shè)計(jì)的指導(dǎo)理論,為支架設(shè)計(jì)提供科學(xué)的依據(jù);(2)設(shè)計(jì)了一種新型雙相三維多孔梯度支架,并驗(yàn)證支架設(shè)計(jì)科學(xué)性,為組織工程應(yīng)用的發(fā)展做了進(jìn)一步科學(xué)探索和貢獻(xiàn)。
[Abstract]:Tissue engineering has a small immune response, which can better reconstruct the original tissue morphology. Structural and functional advantages. The application of osteochondral tissue engineering in the treatment of joint injury has become an effective approach. The core of tissue engineering is the establishment of three-dimensional space complex composed of cells and scaffolds. The scaffold provides a place for cells to transmit nutrients and grow and metabolize, It is also the basis for the formation of new tissues and organs with shape and function. However, there is no complete scaffold design theory to guide the scaffold design, and the scaffold system lacks the vertical level functional gradient change. In view of the defects of the above scaffold design, the main goal of this paper is to establish the scaffold design theory. To provide a scientific research scheme for the study of scaffold design system, a new type of biphasic gradient three-dimensional porous scaffold is designed to meet the various needs of the scaffold in the process of repairing damage. The main work of this paper is to apply the FCBPSSS-Function-Context-Behavior-Principle-State-Structure Theory to the field of scaffold design in tissue engineering. For the tissue repair system, a conceptual scaffold design theory is proposed. The biphasic gradient scaffold was prepared by using 3D biometric printing technology, and the performance of the scaffold was tested. The main conclusion of this paper is that the FCBPSS theory can be used to scientifically and effectively guide the design of scaffolds in bone cartilage repair system. The hydrogel system composed of gelatin / hyaluronic acid Gelma / HA was used as the scaffold cartilage phase, and the poly (lactic acid-glycolic acid / poly (ethylene glycol) / PLGA / PEG) composite system was used as the scaffold bone phase. It can be used for 3D printing scaffold preparation, and is suitable for cell growth, proliferation and differentiation. The scaffold designed with gradient structure in this paper can meet the needs of human mechanical environment strength. The main contribution of this paper is in the aspect of scaffold design theory. Taking the FCBPSS theory as the guiding theory of support design and providing a scientific basis for the design of scaffolds, a new type of biphasic three-dimensional porous gradient scaffold is designed, and the scientific design of the scaffold is verified. Further scientific exploration and contribution have been made for the development of tissue engineering application.
【學(xué)位授予單位】：華東理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08

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