本文選題：組織液　切入點(diǎn)：葡萄糖濃度　出處：《天津大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：血糖檢測在糖尿病的診斷治療中具有重要作用。傳統(tǒng)的取血檢測疼痛感強(qiáng),測量結(jié)果不連續(xù);無創(chuàng)血糖檢測依靠光與人體特定部位組織的相互作用來檢測血糖,個(gè)體差異性強(qiáng),信號提取困難;微創(chuàng)血糖檢測通過檢測組織液中葡萄糖濃度來預(yù)測血糖濃度,可實(shí)現(xiàn)動(dòng)態(tài)、連續(xù)檢測,是目前的研究熱點(diǎn)。然而,受人體生理活動(dòng)的影響,組織液中的葡萄糖濃度與血糖濃度存在一定延遲。在研究這一延遲關(guān)系時(shí),需要引入二者之間的生理延遲參數(shù),建立延遲模型,而延遲模型需要通過精確測量組織液中的葡萄糖濃度和血糖濃度來進(jìn)行實(shí)驗(yàn)驗(yàn)證。但是,如何連續(xù)測量組織液中葡萄糖濃度的精確值目前仍是一個(gè)世界難題。 本文研究了基于微透析的組織液中葡萄糖濃度連續(xù)檢測方法,建立了實(shí)驗(yàn)系統(tǒng),開展了體外模擬葡萄糖濃度連續(xù)變化的檢測實(shí)驗(yàn)和動(dòng)物在體實(shí)驗(yàn),分析了灌流速度、葡萄糖濃度和溫度等主要因素對微透析回收率的影響,并通過超濾方法驗(yàn)證了微透析方法用于連續(xù)檢測組織液中葡萄糖濃度的準(zhǔn)確性。具體工作包括: (1)從理論上研究了采用微透析連續(xù)檢測組織液中葡萄糖濃度的方法,分析了微透析回收率的數(shù)學(xué)模型,討論了測量微透析回收率的方法。 (2)建立了基于微透析的葡萄糖濃度檢測系統(tǒng),利用葡萄糖溶液研究了基于微透析的葡萄糖濃度檢測方法,分析了灌流速度、葡萄糖濃度以及溫度等主要因素對微透析回收率的影響。 (3)建立了模擬人體組織液中葡萄糖濃度連續(xù)變化的實(shí)驗(yàn)平臺,為驗(yàn)證基于微透析的葡萄糖濃度檢測技術(shù)及儀器奠定了基礎(chǔ)。 (4)進(jìn)行了基于超濾抽取的組織液中葡萄糖濃度檢測方法的研究,分析了真空負(fù)壓、探針膜長和連接管長度等對抽取速率的影響,為開展超濾動(dòng)物實(shí)驗(yàn)奠定了基礎(chǔ)。 (5)進(jìn)行了動(dòng)物體內(nèi)的微透析和超濾檢測實(shí)驗(yàn),研究了微透析體內(nèi)回收率的測量方法,并通過超濾方法驗(yàn)證了微透析檢測的準(zhǔn)確性,為組織液中葡萄糖濃度與血糖濃度延遲模型的研究奠定了基礎(chǔ)。
[Abstract]:Blood glucose detection plays an important role in the diagnosis and treatment of diabetes mellitus. The traditional blood sampling test has strong pain, and the measurement results are not continuous. Non-invasive blood glucose detection depends on the interaction between light and specific parts of the human body to detect blood sugar. It is difficult to extract the signal. Microinvasive blood glucose detection can predict glucose concentration by detecting glucose concentration in tissue fluid, which can realize dynamic and continuous detection, which is a hot research topic at present. However, it is affected by physiological activities of human body. There is a certain delay between glucose concentration and blood glucose concentration in tissue solution. In order to study the relationship between glucose concentration and blood glucose concentration, we should introduce the physiological delay parameters between them and establish a delay model. The delay model needs to be verified by accurate measurement of glucose concentration and blood glucose concentration in tissue solution. However, how to continuously measure glucose concentration in tissue fluid is still a difficult problem all over the world. In this paper, the method of continuous measurement of glucose concentration in tissue fluid based on microdialysis was studied, and the experimental system was established. The in vitro and in vivo experiments of simulating the continuous variation of glucose concentration were carried out, and the perfusion rate was analyzed. The effects of glucose concentration and temperature on the recovery rate of microdialysis were studied. The accuracy of microdialysis for continuous determination of glucose concentration in tissue solution was verified by ultrafiltration. 1) the method of continuous determination of glucose concentration in tissue solution by microdialysis was studied theoretically, the mathematical model of recovery rate of microdialysis was analyzed, and the method of measuring the recovery rate of microdialysis was discussed. The glucose concentration detection system based on microdialysis was established. The glucose concentration detection method based on microdialysis was studied by using glucose solution, and the perfusion rate was analyzed. The effect of glucose concentration and temperature on the recovery rate of microdialysis. (3) an experimental platform was established to simulate the continuous variation of glucose concentration in human tissue solution, which laid a foundation for the verification of glucose concentration detection technology and instrument based on microdialysis. The method of measuring glucose concentration in tissue solution based on ultrafiltration was studied. The effects of vacuum negative pressure, the length of probe membrane and the length of connecting pipe on the extraction rate were analyzed, which laid a foundation for the experiment of ultrafiltration animals. In this paper, microdialysis and ultrafiltration tests in animals were carried out, and the method of measuring the recovery rate in vivo was studied, and the accuracy of microdialysis detection was verified by ultrafiltration method. It lays a foundation for the study of the delayed model of glucose concentration and blood glucose concentration in tissue solution.
【學(xué)位授予單位】：天津大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R446.1;TH776

【參考文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 于平;基于微透析技術(shù)的葡萄糖檢測系統(tǒng)[D];浙江大學(xué);2001年

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本文編號：1638508