本文選題：止血帶　切入點：缺血再灌注損傷　出處：《南方醫(yī)科大學學報》2014年07期 　論文類型：期刊論文

【摘要】：目的探討止血帶性缺血再灌注損傷后血小板線粒體的變化,為臨床止血帶的應用提供指導依據。方法 30只SD大鼠隨機分為對照組(6只),缺血再灌注組(24只,再隨機均分為4組,分別在再灌注后2、6、12、24 h取血檢測)。采用自制止血帶環(huán)繞白兔大腿根部(壓力280 mmHg)模擬止血帶性缺血,4h后松開止血帶。相應時間點取血分離血小板。采用全自動血球計數儀測定血小板數量;熒光素—熒光素酶試劑盒測定血小板ATP含量;JC-1線粒體膜電位試劑盒探測線粒體膜電位(△ym)變化;細胞色素C凋亡試劑盒檢測血小板胞質細胞色素C含量;過氧化脂質試劑盒檢測血小板中過氧化脂質。結果與對照組比較,缺血再灌注損傷組血小板內ATP含量在2、6 h顯著降低(P0.05或P0.01),而低線粒體膜電位的血小板含量、胞質細胞色素C含量、過氧化脂質含量均在2、6 h逐漸增加并具有統(tǒng)計學差異(P0.05或P0.01);在12 h時,以上改變有所恢復但仍有統(tǒng)計學差異(P0.05或P0.01);24 h時基本恢復正常(P0.05)。血小板計數在兩組間無統(tǒng)計學差異(P0.05)。結論止血帶性缺血再灌注損傷可以導致血小板線粒體功能的受損,且主要發(fā)生在缺血再灌注早期(6 h),在后期逐漸恢復,而對血小板數量無明顯影響。
[Abstract]:Objective to investigate the changes of platelet mitochondria after tourniquet ischemia-reperfusion injury and to provide guidance for clinical application of tourniquet. Methods Thirty Sprague-Dawley rats were randomly divided into control group (n = 6) and ischemia reperfusion group (n = 24). They were randomly divided into 4 groups. The blood samples were taken at 2: 6, 12, 12 and 24 hours after reperfusion. The tourniquet was loosened after 4 hours of simulated tourniquet ischemia with self-made tourniquet around the root of the rabbit's thigh (pressure 280 mm Hg). Blood platelets were collected at the corresponding time point. Automatic blood cells were used. The platelet count was measured by the counter. The changes of mitochondrial membrane potential (MMP) and cytochrome C (cytochrome C) in platelets were detected by fluorescein luciferase assay kit and JC-1 mitochondrial membrane potential kit respectively. Results compared with the control group, the platelet ATP content in the ischemia-reperfusion injury group decreased significantly at 2 h after reperfusion (P 0.05 or P 0.01), but the platelet content in the low mitochondrial membrane potential was lower than that in the control group. The content of cytochrome C and lipid peroxide in cytoplasm increased gradually at 2h, and there was significant difference between them (P0.05 or P0.01g), and at 12 h, the content of cytochrome C and lipid peroxide increased gradually. The above changes were recovered, but there were statistical differences between the two groups at 24 h after 24 h, the platelet count had no significant difference (P 0.05). Conclusion tourniquet ischemia-reperfusion injury can lead to the damage of platelet mitochondrial function. It mainly occurred at the early stage of ischemia reperfusion and recovered gradually at the later stage, but had no significant effect on platelet count.
【作者單位】： 廣東省人民醫(yī)院麻醉科;廣東省醫(yī)學科學院;
【基金】：廣東省科技計劃項目(2011B031800149,2010B031600252)
【分類號】：R608

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