發(fā)布時(shí)間：2018-03-19 08:25

  本文選題：軟骨細(xì)胞　切入點(diǎn)：Ⅰ型膠原支架材料　出處：《中國(guó)矯形外科雜志》2015年18期 　論文類型：期刊論文

【摘要】：[目的]比較體外靜態(tài)與動(dòng)態(tài)培養(yǎng)兩種方式對(duì)培養(yǎng)人關(guān)節(jié)軟骨細(xì)胞復(fù)合Ⅰ型膠原支架材料的效果,從而探索體外構(gòu)建移植物用于治療關(guān)節(jié)軟骨缺損的適宜條件及方式。[方法]分離培養(yǎng)人關(guān)節(jié)軟骨細(xì)胞,P2代軟骨細(xì)胞接種于Ⅰ型膠原支架,隨機(jī)分為3組,A組:靜態(tài)培養(yǎng);B組:動(dòng)態(tài)培養(yǎng);C組:單層培養(yǎng)。采用倒置相差顯微鏡、熒光顯微鏡、SEM、HE染色觀察細(xì)胞在支架上的分布、黏附、生長(zhǎng)及形態(tài)特點(diǎn),實(shí)時(shí)熒光定量PCR檢測(cè)軟骨細(xì)胞表型特異基因Ⅱ型膠原的表達(dá)情況。[結(jié)果]體外單層培養(yǎng)的軟骨細(xì)胞(P2)以多角形為主,Ⅱ型膠原蛋白表達(dá)陽(yáng)性,提示P2代細(xì)胞表型維持良好;與A組比較,B組細(xì)胞數(shù)量較多且分布均勻,細(xì)胞形態(tài)較均一,以多角形為主;A、B組樣本大體結(jié)構(gòu)及內(nèi)部孔隙結(jié)構(gòu)均保持完整,孔隙內(nèi)均可見(jiàn)細(xì)胞黏附,B組孔隙內(nèi)細(xì)胞數(shù)量及細(xì)胞外基質(zhì)優(yōu)于A組;A、B組的Ⅱ型膠原基因mRNA表達(dá)水平較單層培養(yǎng)組明顯增加。[結(jié)論]軟骨細(xì)胞復(fù)合Ⅰ型膠原支架體外動(dòng)態(tài)培養(yǎng)較靜態(tài)培養(yǎng)可明顯促進(jìn)細(xì)胞增殖及細(xì)胞外基質(zhì)分泌。因此,動(dòng)態(tài)培養(yǎng)有望成為體外構(gòu)建自體軟骨細(xì)胞移植術(shù)所需移植物的一種有效方法。
[Abstract]:[objective] to compare the effects of static and dynamic culture in vitro on cultured human articular chondrocytes combined with type I collagen scaffolds. Therefore, to explore the suitable conditions and methods of constructing grafts for the treatment of articular cartilage defects in vitro. [methods] chondrocytes of P2 generation were isolated from cultured human articular chondrocytes and inoculated on type I collagen scaffolds. Three groups were randomly divided into three groups: static culture group B: dynamic culture group C: monolayer culture. The distribution, adhesion, growth and morphological characteristics of the cells on the scaffold were observed by inverted phase contrast microscope and fluorescence microscope SEMHE staining. The expression of phenotypic specific gene type 鈪，

本文編號(hào)：1633444