本文選題：摻鍶羥基磷灰石　切入點：聚乳酸多孔支架　出處：《河北大學》2017年碩士論文　論文類型：學位論文

【摘要】：骨缺損是臨床上的常見病,也是骨科治療的難題。隨著人類對自身健康的關(guān)注度隨經(jīng)濟發(fā)展的提高,生物醫(yī)用材料產(chǎn)業(yè)高速發(fā)展。組織工程是應用生命科學和工程學的原理,在體外構(gòu)建一個有生物活性的三維空間復合物,作為替代物修復或重建器官,從而達到提高生存質(zhì)量和延長生命活動的目的。目前,傳統(tǒng)生物醫(yī)學材料生物學性能的改進和提高是生物醫(yī)用材料研究的重點。羥基磷灰石(HA)具有極好的生物相容性和骨傳導性,鍶(Sr)具有骨誘導性,Sr摻雜的HA(Sr-HA)作為一種新的生物材料可以顯著促進MC3T3-E1細胞增殖礦化。考慮到骨的成分是有機無機混合材料,制備聚合物/無機復合支架材料具有十分重要的意義。在以往的聚合物/HA復合支架的制備中,一般都是直接將聚合物與HA粒子混合,這種方法會使大部分生物活性HA包埋在聚合物基體內(nèi),并且會造成HA在聚合物基體內(nèi)分布不均,導致在植入早期HA無法與種子細胞有效接觸。本文采用仿生礦化的方法在聚乳酸(PLLA)多孔支架表面沉積摻Sr的HA,制備出仿生礦化的Sr-HA/PLLA多孔支架。這種復合材料表面含有大量的生物無機礦物質(zhì),為細胞的黏附和生長提供了更適宜的環(huán)境,且更好的模擬天然骨的細胞外基質(zhì)成分和結(jié)構(gòu),為骨修復提供一個較好的生物相容環(huán)境。通過掃描電鏡(SEM)、紅外光譜(FT-IR)、XRD、抗壓性能、接觸角和蛋白吸附實驗對其進行表征,結(jié)果表明Sr-HA的沉積可以顯著改善PLLA材料的疏水性,Sr的摻入明顯增加材料的骨誘導性,與傳統(tǒng)的HA相比,這種復合材料具有更高的成骨性能和更優(yōu)良可控的降解性能。體內(nèi)和體外實驗都表明復合材料的生物相容性良好,促進成骨細胞黏附、增殖和分化,并且誘導骨修復。
[Abstract]:Bone defect is not only a common disease in clinic, but also a difficult problem in orthopedic treatment. With the development of economy, the biomedical material industry develops rapidly. Tissue engineering is the application of life science and engineering principle. Construct a bioactive three-dimensional complex in vitro to repair or reconstruct the organ as an alternative, thereby improving the quality of life and prolonging life activities. The improvement and improvement of biological properties of traditional biomedical materials is the focus of biomedical materials research. Hydroxyapatite (HA) has excellent biocompatibility and bone conductivity. As a new biomaterial, Sr) can significantly promote the proliferation and mineralization of MC3T3-E1 cells, considering that the bone is an organic and inorganic mixture. The preparation of polymer / inorganic composite scaffolds is of great significance. In the past polymer / HA composite scaffolds were prepared by mixing polymer directly with HA particles. In this way, most of the bioactive HA is embedded in the polymer group, and the HA is unevenly distributed in the polymer group. In this paper, the biomimetic mineralization method was used to deposit Sr-doped Sr-HA/PLLA scaffold on the porous scaffold of polylactic acid (PLLA), and the biomimetic mineralized Sr-HA/PLLA scaffold was prepared. Containing a lot of biological inorganic minerals, It provides a more suitable environment for cell adhesion and growth, and better mimics the extracellular matrix composition and structure of natural bone, and provides a better biocompatibility environment for bone repair. The results of contact angle and protein adsorption experiments showed that the deposition of Sr-HA could significantly improve the osseoinductivity of PLLA materials by incorporation of hydrophobicity and Sr, compared with the traditional HA. Both in vivo and in vitro experiments show that the composite has good biocompatibility, promotes osteoblast adhesion, proliferation and differentiation, and induces bone repair.
【學位授予單位】：河北大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R318.08

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