本文選題：軟骨疾病/治療　切入點(diǎn)：硅　出處：《浙江大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2016年02期 　論文類(lèi)型：期刊論文

【摘要】：目的:初步構(gòu)建基于軟骨鈣化層損傷重建的有機(jī)—無(wú)機(jī)復(fù)合組織工程支架,探究摻鎂硅灰石含量與支架抗壓性能之間的關(guān)系。方法:利用質(zhì)量分?jǐn)?shù)分別為1%、3%、5%的高生物活性鈣鎂硅酸鹽超細(xì)顆粒復(fù)合Ⅰ型膠原—透明質(zhì)酸鈉進(jìn)行三維打印,經(jīng)海藻酸鈉—氯化鈣氣霧交聯(lián)成型,電鏡下觀察表面孔隙、孔徑、無(wú)機(jī)相分布,萬(wàn)能材料試驗(yàn)機(jī)測(cè)試抗壓性能,并計(jì)算支架孔隙率。結(jié)果:支架表面平均孔徑(212.3±34.2)μm,平均孔隙率(48.3±5.9)%,不同質(zhì)量分?jǐn)?shù)的高生物活性鈣鎂硅酸鹽超細(xì)顆粒復(fù)合Ⅰ型膠原—透明質(zhì)酸鈉支架壓縮模量差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),平均壓縮模量(7.2±1.2)MPa,介于軟骨和軟骨下骨之間。結(jié)論:利用三維打印技術(shù)成功構(gòu)建出多孔鈣化層仿生重建支架,可為今后研制多層次復(fù)合支架治療骨—軟骨損傷奠定基礎(chǔ)。
[Abstract]:Objective: to construct an organic-inorganic composite tissue engineering scaffold based on cartilage calcification layer injury reconstruction. The relationship between the content of magnesium-doped wollastonite and the compressive resistance of the scaffold was investigated. Methods: Three-dimensional printing was carried out by using the ultrafine particles of high bioactive calcium magnesium silicate ultrafine particles, type I collagen-sodium hyaluronate, with a mass fraction of 1% or 3%, respectively. The pore size, pore size and distribution of inorganic phase on the surface were observed under electron microscope by crosslinking of sodium alginate and calcium chloride, and the compressive resistance was tested by universal material testing machine. Results: the average pore size of scaffold was 212.3 鹵34.2 渭 m and the average porosity was 48.3 鹵5.9 渭 m. There was no difference in compression modulus of high bioactive calcium magnesium silicate ultrafine particle composite type I collagen-sodium hyaluronate scaffold with different mass fraction. The mean compression modulus is 7.2 鹵1.2 mpa, which is between cartilage and subchondral bone. Conclusion: the biomimetic reconstruction scaffold of porous calcified layer was successfully constructed by three-dimensional printing technique. It can lay a foundation for the treatment of bone-cartilage injury with multi-layer composite scaffold in the future.
【作者單位】： 浙江大學(xué)醫(yī)學(xué)院附屬第二醫(yī)院骨科浙江大學(xué)骨科研究所;浙江加州國(guó)際納米技術(shù)研究院;
【基金】：國(guó)家自然科學(xué)基金(81472063) 浙江省自然科學(xué)基金(LZ14E020001)
【分類(lèi)號(hào)】：R687;R318.08
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本文編號(hào)：1627285