本文選題：水熱法　切入點：等離子體浸沒離子注入　出處：《湖南大學(xué)》2012年博士論文　論文類型：學(xué)位論文

【摘要】：鈦具有良好的生物相容性,被廣泛用作硬組織替換材料。但是,鈦是生物惰性材料,植入人體內(nèi)易被當做異物。對醫(yī)用鈦進行表面改性,賦予醫(yī)用鈦理想的耐腐性能、生物活性和抗菌性,可有效改善其植入效果。本論文利用銀/氮二元離子注入對鈦進行表面改性,探查了銀/氮二元離子注入順序?qū)︹伇砻娴募{米硬度、耐腐能力和抗菌能力的影響。結(jié)合水熱法和等離子體浸沒離子注入技術(shù),對醫(yī)用鈦進行表面改性,探查了改性鈦表面的納米結(jié)構(gòu)、生物活性、抗菌性能和細胞相容性。此外,初步探討了溶膠凝膠法制備聚乳酸(PLA)/CaO-Si02復(fù)合薄膜的生物活性和生物相容性。取得的主要研究成果如下： 1.利用等離子體浸沒離子注入技術(shù)將銀/氮二元離子按不同順序(即先銀后氮、先氮后銀和銀氮共注)注入鈦的表面。銀/氮二元離子注入鈦表面的銀以單質(zhì)形式存在,而氮以氮化鈦的形式存在。不同的銀/氮二元注入順序?qū)Ω男院筲伒哪透�、納米硬度和抗菌性能均有影響。銀/氮二元離子共注鈦表面的耐腐性能和硬度均優(yōu)于先氮后銀和先銀后氮注入鈦表面,這是因為銀/氮二元共注導(dǎo)致的熱效應(yīng)使鈦表面生成的氮化層最厚。銀/氮二元注入樣品對大腸桿菌和金黃色葡萄球菌均具有良好的抗菌作用。細胞實驗表明,銀/氮二元注入樣品對細胞沒有明顯毒性,且有利于細胞的增殖和分化。 2.采用雙氧水水熱法在鈦表面預(yù)先構(gòu)建元素主要成份為Ti和O的不同納米結(jié)構(gòu)。利用等離子體浸沒離子注入技術(shù)將銀負載在雙氧水水熱處理鈦表面,注銀后樣品表面納米結(jié)構(gòu)仍然保留完好。相對于純鈦片,注銀前后鈦基材的耐腐能力均獲得了明顯的改善。模擬體液浸泡實驗顯示,注銀前后鈦表面均具有良好的生物活性。注銀樣品對大腸桿菌和金黃色葡萄球菌繁殖具有明顯抑制作用,顯示出良好的抗菌性。細胞毒性實驗表明,水熱處理72小時鈦表面注銀前后對小鼠成骨細胞的繁殖和分化均具有良好的促進作用。 3.以氫氧化鈉和雙氧水作為反應(yīng)介質(zhì),在鈦表面構(gòu)建了四種納米結(jié)構(gòu),采用等離子體浸沒離子注入技術(shù)將銀負載在納米結(jié)構(gòu)表面。模擬體液浸泡實驗顯示,注銀前后鈦表面均顯示良好的生物活性。注銀表面對大腸桿菌和金黃色葡萄球菌均具有良好的抗菌能力。細胞毒性實驗表明,除氫氧化鈉水熱處理并注銀樣品外,其它三種水熱處理并注銀鈦表面對小鼠成骨細胞的增殖和分化具有較好的促進作用。 4.制備了不同比例的PLA/CaO-Si02多孔復(fù)合薄膜。在模擬體液浸泡7天的薄膜表面形成環(huán)狀結(jié)構(gòu)的類骨磷灰石層,顯示良好生物活性。隨著CaO-SiO2含量增加,復(fù)合薄膜表面微孔的孔徑變小,薄膜表面zeta電位變負,誘導(dǎo)類骨磷灰石沉積的能力增強；MTT實驗證實復(fù)合薄膜對MG-63細胞沒有毒性且有利于細胞的增殖。
[Abstract]:Titanium has good biocompatibility and is widely used as a substitute for hard tissue. However, titanium is a biological inert material and is easily used as a foreign body in human body. In this paper, the surface modification of titanium was carried out by silver / nitrogen binary ion implantation, and the nano-hardness of titanium surface was investigated by silver / nitrogen binary ion implantation sequence. Combined with hydrothermal method and plasma immersion ion implantation technology, the surface modification of medical titanium was carried out, and the nanostructure, bioactivity, antibacterial property and cell compatibility of the modified titanium surface were investigated. The bioactivity and biocompatibility of poly (lactic acid) / CaO-Si02 composite films prepared by sol-gel method were preliminarily discussed. The main results were as follows:. 1. Silver / nitrogen binary ions were implanted into titanium surface by plasma immersion ion implantation in different order (that is, first silver then nitrogen, first nitrogen then silver and silver nitrogen co-implantation). However, nitrogen exists in the form of titanium nitride. Both nano-hardness and antimicrobial properties were affected. The corrosion resistance and hardness of Ag / N co-implanted titanium surface were better than that of Ag and Ag implanted titanium surface. This is because the thermal effect of silver / nitrogen binary injection results in the thickest nitrided layer on the surface of titanium. The silver / nitrogen binary implanted sample has good antibacterial effect on both Escherichia coli and Staphylococcus aureus. The silver / nitrogen binary implanted sample had no obvious toxicity to cells and was beneficial to cell proliferation and differentiation. 2. Different nanostructures with main elements Ti and O were pre-constructed on titanium surface by hydrogen peroxide hydrothermal method. Silver was loaded on titanium surface after heat treatment by plasma immersion ion implantation. The surface nanostructures of the samples remained intact after silver injection. Compared with pure titanium sheets, the corrosion resistance of titanium substrates before and after silver implantation was improved obviously. The simulated body fluid immersion test showed that the corrosion resistance of titanium substrates before and after silver injection was significantly improved. The surface of titanium has good biological activity before and after silver injection. The silver injected sample has obvious inhibitory effect on the reproduction of Escherichia coli and Staphylococcus aureus. After 72 hours of hydrothermal treatment, silver implantation on titanium surface could promote the proliferation and differentiation of mouse osteoblasts. 3. Using sodium hydroxide and hydrogen peroxide as the reaction medium, four kinds of nanostructures were constructed on the surface of titanium. Silver was loaded on the surface of the nanostructure by plasma immersion ion implantation technique. The surface of titanium showed good biological activity before and after silver injection, and the surface of silver injection had good antibacterial activity against Escherichia coli and Staphylococcus aureus. The cytotoxicity test showed that, except for sodium hydroxide hydrothermal treatment and silver injection, the surface of titanium had good antibacterial activity against Escherichia coli and Staphylococcus aureus. The other three kinds of hydrothermal treatment with silver and titanium can promote the proliferation and differentiation of mouse osteoblasts. 4. PLA/CaO-Si02 porous composite films with different proportions were prepared. The porous apatite layer with annular structure was formed on the surface of the membrane immersed in simulated body fluid for 7 days, which showed good biological activity. With the increase of CaO-SiO2 content, the pore size of micropores on the surface of the composite film became smaller. The zeta potential on the surface of the membrane was negative, and the ability to induce osteoid apatite deposition was enhanced. The results showed that the composite membrane was not toxic to MG-63 cells and was beneficial to cell proliferation.
【學(xué)位授予單位】：湖南大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2012
【分類號】：R318.08

【參考文獻】

相關(guān)期刊論文 前6條

1 黃占杰;無機抗菌劑的發(fā)展與應(yīng)用[J];材料導(dǎo)報;1999年02期

2 郭亮,梁成浩,隋洪艷;離子注N對人體用金屬材料在人工模擬體液中的陽極極化行為的影響[J];電化學(xué);2000年02期

3 馮衛(wèi);交鎖髓內(nèi)釘治療脛骨骨折研究進展[J];骨與關(guān)節(jié)損傷雜志;2001年05期

4 墻薔;倪紅衛(wèi);幸偉;謝中;魏勇;;銀的抗菌作用機理[J];武漢科技大學(xué)學(xué)報(自然科學(xué)版);2007年02期

5 李昭成,楊桂花;流動電位法Zeta電位儀的測量原理及使用性能[J];紙和造紙;2002年04期

6 張效忠,張劍強,郭興唐;氮離子注入在鈦合金人工全髖關(guān)節(jié)中的應(yīng)用[J];中國生物醫(yī)學(xué)工程學(xué)報;1988年04期

相關(guān)碩士學(xué)位論文 前1條

1 苑春;載銀二氧化鈦復(fù)合微粉的制備及抗菌性能表征[D];中南大學(xué);2005年

，

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