發(fā)布時(shí)間：2018-03-05 23:12

  本文選題：細(xì)胞外基質(zhì)　切入點(diǎn)：堿活化　出處：《西南交通大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：鈦是一種具有良好生物相容性、機(jī)械性能及抗腐蝕性能的生物材料,廣泛應(yīng)用于研究心血管疾病領(lǐng)域。目前,生物材料表面改性成為加速內(nèi)皮化,預(yù)防血管再狹窄的一大熱題。研究表明生物材料的表面形貌、官能團(tuán)結(jié)構(gòu)對(duì)內(nèi)皮細(xì)胞的粘附及增殖有促進(jìn)作用,并且能夠誘導(dǎo)細(xì)胞分泌更多的細(xì)胞外基質(zhì),從而改善材料表面生物相容性。內(nèi)皮細(xì)胞自分泌的細(xì)胞外基質(zhì)含有對(duì)細(xì)胞粘附、生長(zhǎng)、增殖有促進(jìn)作用的多種成份,因此將鈦堿活化、等離子體聚合烯丙胺、表面沉積多巴胺與細(xì)胞自分泌的細(xì)胞外基質(zhì)相結(jié)合應(yīng)用于鈦表面改性,充分改善材料表面的生物相容性。 本實(shí)驗(yàn)分別在鈦箔(Ti)以及堿活化鈦箔(Ti-OH)、等離子體聚合烯丙胺薄膜(Ti-PPAam)、聚多巴胺薄膜(Ti-DA)改性的鈦箔表面,由內(nèi)皮細(xì)胞(EC)原位沉積細(xì)胞外基質(zhì)(ECM)。接觸角檢測(cè)結(jié)果表明,沉積ECM使得Ti-OH和Ti-PPAam的接觸角均有所提高,Ti-DA的接觸角則減小19°。掃描電鏡結(jié)果顯示,除了光滑鈦箔外,其它鈦箔界面均形成一層均勻的交聯(lián)狀的ECM結(jié)構(gòu)。傅里葉變換紅外光譜(FTIR)檢測(cè)結(jié)果表明,Ti-OH表面富含大量羥基,Ti-PPAam及Ti-DA表面含大量-NH2,各組樣品經(jīng)ECM改性后,都在1650cm-1處有-C=O官能團(tuán)吸收峰,表明細(xì)胞外基質(zhì)的成功構(gòu)建。千萬(wàn)分之一天平精確稱量并計(jì)算出各組鈦箔表面ECM改性前后的質(zhì)量差,發(fā)現(xiàn)多孔親水性的Ti-OH表面ECM含量最多,達(dá)到0.075mmg,與其他兩組相比具有顯著性差異(P0.05)。 體外EC粘附及增殖結(jié)果表明：Ti-OH表面、Ti-PPAam表面對(duì)EC有一定的抑制作用,經(jīng)EC-ECM改性后,細(xì)胞相容性有一定的提高,而ECM改性的Ti-DA表面相比其他兩組,細(xì)胞相容性更好(P0.05)。體外平滑肌細(xì)胞(SMC)的粘附及增殖結(jié)果表明：經(jīng)EC-ECM改性后,各組樣品的SMC粘附數(shù)量及活性均有顯著性提高。內(nèi)皮細(xì)胞NO釋放結(jié)果表明：各組樣品經(jīng)EC-ECM改性后均促進(jìn)EC的一氧化氮(NO)釋放,且聚多巴胺薄膜與EC-ECM共同促進(jìn)EC釋放NO,有利于保持EC的功能和表型。體外血小板粘附結(jié)果表明：堿活化表面改性前后均明顯抑制血小板粘附和聚集(P0.05),等離子體聚合烯丙胺表面血小板粘附和激活較嚴(yán)重,而聚多巴胺薄膜在抑制血小板粘附和激活的同時(shí)具有相對(duì)良好的細(xì)胞相容性,并且經(jīng)細(xì)胞外基質(zhì)改性后,進(jìn)一步提高了血液相容性和細(xì)胞相容性。 本研究結(jié)果表明,在以聚多巴胺為基底的鈦表面原位構(gòu)建EC-ECM,是改善材料表面生物相容性的有效方法。
[Abstract]:Titanium is a kind of biomaterial with good biocompatibility, mechanical properties and corrosion resistance, which is widely used in the field of cardiovascular disease. Studies have shown that the surface morphology and functional group structure of biomaterials can promote the adhesion and proliferation of endothelial cells, and induce the cells to secrete more extracellular matrix. In order to improve the biocompatibility on the surface of the material, the extracellular matrix secreted by endothelial cells contains many components which can promote cell adhesion, growth and proliferation, so the titanium base was activated and plasma polymerized allylamine. Dopamine deposited on the surface was combined with extracellular matrix (ECM) to improve the biocompatibility of titanium. In this experiment, the extracellular matrix (ECM) was deposited in situ from endothelial cells on the surface of titanium foil modified by titanium foil (Ti), alkaline activated titanium foil (Ti-OHN), plasma polymerized allylamine film (Ti-PPAamn) and polydopamine film (Ti-DA). The results of contact angle measurement showed that, The contact angle of Ti-OH and Ti-PPAam was increased by deposition of ECM, and the contact angle of Ti-DA decreased by 19 擄. The SEM results showed that, except for smooth titanium foil, The results of Fourier transform infrared spectroscopy (FTIR) show that the surface of Ti-OH is rich in a large amount of hydroxyl group Ti-PPAam and the surface of Ti-DA contains a large amount of -NH _ 2. The samples were modified by ECM. At 1650 cm ~ (-1), the absorption peak of C _ (10) O functional group was found, which indicated that the extracellular matrix was successfully constructed. The accurate weighing of 1/10000000 scales and the calculation of the mass difference before and after modification of ECM on each group of titanium foil surfaces showed that the surface ECM content of porous hydrophilic Ti-OH was the highest. Compared with the other two groups, there was a significant difference between the two groups (P 0.05). The results of EC adhesion and proliferation in vitro showed that Ti-PPAam surface of W Ti-OH surface could inhibit EC to some extent, and the cytocompatibility of Ti-DA modified by EC-ECM was improved to a certain extent, while that of Ti-DA modified with ECM was higher than that of the other two groups. The results of adhesion and proliferation of smooth muscle cells (SMC) in vitro were as follows: modified by EC-ECM, The number and activity of SMC adhesion were significantly increased in each group. The results of no release from endothelial cells showed that the release of nitric oxide (no) from EC was promoted by EC-ECM modification in each group. Poly (dopamine) film and EC-ECM can promote the release of no from EC, which is beneficial to maintain the function and phenotype of EC. The results of platelet adhesion in vitro showed that platelet adhesion and platelet aggregation (P0.05N) were significantly inhibited before and after surface modification by alkaline activation, and plasma aggregation was observed. The adhesion and activation of platelet on the surface of allylamine were serious. The membrane of polydopamine has good cytocompatibility while inhibiting platelet adhesion and activation, and after modified by extracellular matrix, it can further improve the blood compatibility and cytocompatibility. The results show that in situ construction of EC-ECM on the surface of titanium based on polydopamine is an effective method to improve the biocompatibility of the surface.
【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 肖琳琳;魏雨;計(jì)劍;;基于聚多巴胺輔助自組裝單分子層技術(shù)的PTFE表面修飾及其內(nèi)皮細(xì)胞選擇性黏附研究[J];高分子學(xué)報(bào);2010年04期

2 李建樹(shù),羅祥林,何成生,樊翠蓉,鐘銀屏;高分子材料表面固定生物分子的光化學(xué)方法[J];高分子材料科學(xué)與工程;2001年05期

3 陳放,吳洪才;離子注入對(duì)聚合物材料表面改性的研究與應(yīng)用進(jìn)展[J];高分子材料科學(xué)與工程;2005年03期

4 胡大海,陳璧;組織修復(fù)的再生生物學(xué)與工程學(xué)[J];國(guó)外醫(yī)學(xué).生物醫(yī)學(xué)工程分冊(cè);2000年03期

5 湯順清,周長(zhǎng)忍,鄒翰;生物材料的發(fā)展現(xiàn)狀與展望(綜述)[J];暨南大學(xué)學(xué)報(bào)(自然科學(xué)與醫(yī)學(xué)版);2000年05期

6 宋立群;牙周骨組織缺損修復(fù)的生物材料研究進(jìn)展[J];口腔材料器械雜志;2003年02期

7 李愛(ài)民,孫康寧,尹衍升,盧志華,王振光,畢見(jiàn)強(qiáng);生物材料的發(fā)展、應(yīng)用、評(píng)價(jià)與展望[J];山東大學(xué)學(xué)報(bào)(工學(xué)版);2002年03期

8 郭勇;劉璐;郝慶新;李瑞欣;張西正;王亮;寧波;;體外培養(yǎng)所形成的細(xì)胞外基質(zhì)對(duì)MC3T3-E1細(xì)胞生長(zhǎng)和分化的影響[J];生物工程學(xué)報(bào);2011年11期

9 高春華,黃新友;組織工程與生物材料[J];上海生物醫(yī)學(xué)工程;2003年04期

10 王志勇,鄒祖玉,劉維新;血管平滑肌細(xì)胞的表型標(biāo)志α-SM-actin的表達(dá)與調(diào)控的研究進(jìn)展[J];數(shù)理醫(yī)藥學(xué)雜志;2001年05期

，

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