本文選題：富血小板血漿　切入點(diǎn)：復(fù)合生長(zhǎng)因子　出處：《中國(guó)輸血雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的制備載血小板復(fù)合生長(zhǎng)因子明膠緩釋微球,觀察其一般特性,檢測(cè)其體外緩釋效果。方法以明膠為原料,戊二醛為交聯(lián)劑,采用改良的乳化冷凝聚合法制備明膠微球,以微球粒徑為參考指標(biāo),通過(guò)單因素實(shí)驗(yàn)對(duì)明膠微球的制備條件進(jìn)行優(yōu)化。用光學(xué)顯微鏡、掃描電鏡(SEM)對(duì)其大小結(jié)構(gòu)形貌等一般特性進(jìn)行表征;以血管內(nèi)皮生長(zhǎng)因子(vascular epithelial growth factor,VEGF)的含量為代表,酶聯(lián)免疫吸附測(cè)定(ELISA)法檢測(cè)其含量,計(jì)算微球吸附生長(zhǎng)因子率、載生長(zhǎng)因子量,測(cè)定體外緩釋效果。結(jié)果當(dāng)明膠溶液濃度為15%、Span8加入量為1.5 m L,攪拌速度為(750-855)r/min時(shí)可獲得最優(yōu)的明膠微球制備方案,獲得的明膠微球平均粒徑為15.95±3.79μm,其中7-20.5μm的微球占88.23%。制備的PG上清中VEGF的含量為(694.45±1.13)pg/m L,微球吸附生長(zhǎng)因子率為87.7%,載VEGF量為6.09 pg/mg,體外緩釋7 d累計(jì)釋放量占總載藥率的75.73%。結(jié)論制備的明膠緩釋微球外觀圓整,粒徑均勻,分散度良好,吸附生長(zhǎng)因子率、載生長(zhǎng)因子量及體外緩釋效果良好,初步實(shí)現(xiàn)了載血小板復(fù)合生長(zhǎng)因子明膠緩釋微球凍干制品的研制。
[Abstract]:Objective to prepare platelet-loaded composite growth factor gelatin sustained-release microspheres, observe their general characteristics and detect their effects in vitro. Methods Gelatin microspheres were prepared by modified emulsification condensation polymerization with gelatin as raw material and glutaraldehyde as crosslinking agent. The preparation conditions of gelatin microspheres were optimized by single factor experiments. The size and morphology of gelatin microspheres were characterized by optical microscope and scanning electron microscope (SEM). The content of vascular epithelial growth factor (VEGF) was measured by Elisa, and the rate of adsorbed growth factor and the load of VEGF by microspheres were calculated, and the content of VEGFwas determined by enzyme-linked immunosorbent assay (Elisa). Results the optimal preparation scheme of gelatin microspheres was obtained when the concentration of gelatin solution was 1.5 mL and the stirring speed was 750-855 r / min. The average diameter of gelatin microspheres was 15.95 鹵3.79 渭 m, of which 7-20.5 渭 m microspheres accounted for 88.23.The content of VEGF in the supernatant of PG was 694.45 鹵1.13 渭 g / mL, the adsorption growth factor rate of microspheres was 87.7%, the amount of VEGF loaded was 6.09 PG / mg, and the accumulative release amount in vitro was 75.73% of the total drug loading rate. Conclusion the gelatin sustained-release microspheres have a round appearance. The preparation of platelet-loaded composite growth factor gelatin sustained-release microspheres was preliminarily realized with uniform particle size, good dispersion, adsorption growth factor rate, load growth factor content and in vitro sustained release effect.
【作者單位】： 廣州醫(yī)科大學(xué)研究生學(xué)院;廣州軍區(qū)廣州總醫(yī)院輸血科;南方醫(yī)科大學(xué);
【分類號(hào)】：R641;R318.08

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本文編號(hào)：1558255