本文關(guān)鍵詞： 關(guān)節(jié) 軟骨 骨形態(tài)發(fā)生蛋白質(zhì)類 成纖維細(xì)胞生長(zhǎng)因子 組織構(gòu)建 軟骨組織工程 骨形態(tài)發(fā)生蛋白 堿性成纖維細(xì)胞生長(zhǎng)因子 生物材料 關(guān)節(jié)軟骨缺損 生物性能 膝關(guān)節(jié) 軟骨細(xì)胞 修復(fù)材料　出處：《中國(guó)組織工程研究》2016年20期 　論文類型：期刊論文

【摘要】：背景:眾多的實(shí)驗(yàn)中發(fā)現(xiàn)了多種細(xì)胞因子通過自分泌和旁分泌等不同方式調(diào)節(jié)軟骨和骨的生成。目的:分析骨形態(tài)發(fā)生蛋白、堿性成纖維細(xì)胞生長(zhǎng)因子生物材料在關(guān)節(jié)軟骨缺損修復(fù)中的生物性能。方法:選取40只新西蘭家兔,隨機(jī)分為4組,纖維蛋白組、堿性成纖維細(xì)胞生長(zhǎng)因子組、骨形態(tài)發(fā)生蛋白組、復(fù)合組(骨形態(tài)發(fā)生蛋白+堿性成纖維細(xì)胞生長(zhǎng)因子),每組10只。建立兔關(guān)節(jié)軟骨缺損模型,止血徹底后將纖維蛋白、堿性成纖維細(xì)胞生長(zhǎng)因子、骨形態(tài)發(fā)生蛋白以及骨形態(tài)發(fā)生蛋白、堿性成纖維細(xì)胞生長(zhǎng)因子復(fù)合等材料組成的支架分別植入缺損部位。比較不同注射材料在家兔關(guān)節(jié)軟骨缺損中的效果及復(fù)合材料的生物性能。結(jié)果與結(jié)論:(1)關(guān)節(jié)軟骨缺損修復(fù)情況:纖維蛋白組2只家兔出現(xiàn)跛行;堿性成纖維細(xì)胞生長(zhǎng)因子組1只家兔活動(dòng)受限;骨形態(tài)發(fā)生蛋白組出現(xiàn)1只跛行,1只活動(dòng)受限;復(fù)合組兔恢復(fù)良好,膝關(guān)節(jié)和手術(shù)前相比差異無顯著性意義(P0.05)。(2)大體觀察:復(fù)合組家兔軟解軟骨缺損消失,內(nèi)有新生血管,軟骨和正常組織十分接近;骨形態(tài)發(fā)生蛋白組關(guān)節(jié)軟骨邊際存在裂隙,未能與正常的軟骨組織緊密結(jié)合,光鏡下能夠看見缺損區(qū)周緣存在軟骨細(xì)胞;纖維蛋白組缺損部位和周圍組織基本愈合;堿性成纖維細(xì)胞生長(zhǎng)因子組缺損部位有所修復(fù),但不光滑。(3)蘇木精-伊紅染色結(jié)果:纖維蛋白組兔缺損部位未被修復(fù),表面存在明顯凹陷;堿性成纖維細(xì)胞生長(zhǎng)因子組缺損部位被明顯修復(fù),缺損部位存在大量軟骨細(xì)胞;骨形態(tài)發(fā)生蛋白組被修復(fù),出現(xiàn)軟骨細(xì)胞,但排列不規(guī)則;復(fù)合組修復(fù)良好,出現(xiàn)大量軟骨細(xì)胞。(4)結(jié)果提示,骨形態(tài)發(fā)生蛋白、堿性成纖維細(xì)胞生長(zhǎng)因子生物材料是關(guān)節(jié)軟骨缺損中比較理想的修復(fù)材料,能夠發(fā)揮骨形態(tài)發(fā)生蛋白誘導(dǎo)形成軟骨的活性,能夠發(fā)揮堿性成纖維細(xì)胞生長(zhǎng)因子提高軟骨細(xì)胞增生作用,可以達(dá)到優(yōu)勢(shì)互補(bǔ),促進(jìn)關(guān)節(jié)軟骨缺損的恢復(fù)。
[Abstract]:Background: in many experiments, many cytokines have been found to regulate cartilage and bone formation through autocrine and paracrine, etc. Objective: to analyze bone morphogenetic protein (BMP). Biological properties of basic fibroblast growth factor biomaterials in repair of articular cartilage defect methods: 40 New Zealand rabbits were randomly divided into 4 groups: fibrin group, basic fibroblast growth factor group, and fibroblast growth factor group. Bone morphogenetic protein group, composite group (bone morphogenetic protein basic fibroblast growth factor, 10 in each group. Rabbit articular cartilage defect model, after hemostasis thoroughly fibrin, basic fibroblast growth factor, Bone morphogenetic proteins and bone morphogenetic proteins, The scaffolds composed of basic fibroblast growth factor (bFGF) and other materials were implanted into the defect site respectively. The effects of different injection materials on articular cartilage defects in rabbits and the biological properties of composite materials were compared. Repair of cartilage defect: lameness appeared in 2 rabbits in fibrin group; In the basic fibroblast growth factor group, the activity of one rabbit was restricted, the bone morphogenetic protein group showed a limping limitation, and the compound group recovered well. There was no significant difference between the knee joint and the operation before operation (P 0.05). Gross observation: in the composite group, the defect of soft decomposed cartilage disappeared, there were neovascularization in the cartilage, the cartilage and normal tissue were close to each other, and the bone morphogenetic protein group had a crack in the articular cartilage margin. The defect site and surrounding tissue were basically healed in fibrin group, and the defect site was repaired in basic fibroblast growth factor group. However, the results of hematoxylin-eosin staining showed that the defect site was not repaired in fibrin group, and there was a significant depression on the surface of the defect, and a large number of chondrocytes were found in the defect site in basic fibroblast growth factor group. Bone morphogenetic protein group was repaired with irregular arrangement of chondrocytes, and the composite group was well repaired with a large number of chondrocytes. Basic fibroblast growth factor (bFGF) biomaterials are ideal repair materials for articular cartilage defects, which can play the role of bone morphogenetic protein-induced cartilage formation. It can play the role of basic fibroblast growth factor to enhance the proliferation of chondrocytes, to achieve complementary advantages, and to promote the recovery of articular cartilage defects.
【作者單位】： 南陽醫(yī)學(xué)高等�？茖W(xué)校第一附屬醫(yī)院;鄭州大學(xué)第一附屬醫(yī)院;
【基金】：鄭州市科技局科研項(xiàng)目資助(20150138)~~
【分類號(hào)】：R318.08;R684

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