本文關(guān)鍵詞： 組織工程 低溫沉積制造 支架 脊髓損傷 雪旺細(xì)胞　出處：《中國(guó)生物醫(yī)學(xué)工程學(xué)報(bào)》2017年01期 　論文類型：期刊論文

【摘要】：組織工程的迅速興起給脊髓損傷后的神經(jīng)功能恢復(fù)帶來(lái)了新的希望,而三維結(jié)構(gòu)組織工程支架成為用組織工程方法治療脊髓損傷的關(guān)鍵因素。嘗試采用PLGA低溫成形支架修復(fù)脊髓損傷。采用PLGA作為生物支架材料,通過(guò)低溫快速成形工藝制備脊髓支架,采用雪旺細(xì)胞進(jìn)行細(xì)胞培養(yǎng)實(shí)驗(yàn),選用30只SD大鼠分組進(jìn)行支架修復(fù)脊髓損傷的動(dòng)物實(shí)驗(yàn),并對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行組織切片和染色表征。對(duì)支架的各項(xiàng)性能進(jìn)行表征后,結(jié)果表明支架的平均孔隙率達(dá)到87.64%,具有良好的親水性。支架降解速率表現(xiàn)出前期慢、后期加快的趨勢(shì),實(shí)驗(yàn)中支架在前16周表現(xiàn)良好的穩(wěn)定性,從20周起逐步加快。各項(xiàng)性能能滿足組織工程脊髓支架的要求。觀察顯示,雪旺細(xì)胞在PLGA支架上生長(zhǎng)、增殖情況良好,支架細(xì)胞相容性良好,適合細(xì)胞黏附和生長(zhǎng)。BBB評(píng)分顯示,實(shí)驗(yàn)組大鼠截癱情況從第4周起有好轉(zhuǎn)跡象,受傷大鼠后肢觀察到輕微活動(dòng)跡象。不同切片染色觀察結(jié)果表明,該組織工程支架對(duì)于脊髓損傷修復(fù)均有不同程度的促進(jìn),在阻礙膠質(zhì)癜痕生成、促進(jìn)神經(jīng)纖維及髓鞘愈合方面取得不同程度的效果。觀察表明,該方法能夠在脊髓損傷修復(fù)中取得實(shí)質(zhì)性的治療效果。
[Abstract]:The rapid rise of tissue engineering brings new hope to the recovery of neural function after spinal cord injury. Three-dimensional scaffold for tissue engineering has become a key factor in the treatment of spinal cord injury. The PLGA cryogenic scaffold was used to repair spinal cord injury. PLGA was used as biomaterial for spinal cord injury. Spinal cord scaffolds were prepared by low temperature rapid prototyping. Schwann cells were used for cell culture and 30 SD rats were divided into two groups. The results showed that the average porosity of the scaffold was 87.64, and the scaffold had good hydrophilicity. The degradation rate of the scaffold was slow in the early stage and accelerated in the later stage. The stents showed good stability in the first 16 weeks and accelerated gradually from the 20th week. The performance of the scaffolds could meet the requirements of tissue engineering spinal cord scaffolds. The observation showed that Schwann cells grew well on the PLGA scaffold, and the proliferation of Schwann cells was good. The scaffold had good cytocompatibility, suitable for cell adhesion and growth. BBB score showed that the paraplegia in the experimental group showed signs of improvement from the 4th week, and slight signs of movement were observed in the injured hind limbs. The tissue engineering scaffold can promote the repair of spinal cord injury in different degree, and has different effects on blocking the formation of glial scar and promoting the healing of nerve fiber and myelin sheath. This method can achieve substantial therapeutic effect in spinal cord injury repair.
【作者單位】： 杭州電子科技大學(xué)生物制造研究中心;
【基金】：國(guó)家自然科學(xué)基金(61272389,60952001)
【分類號(hào)】：R318.08;R651.2

【參考文獻(xiàn)】

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