本文關(guān)鍵詞： 龍腦 纖維素 殼聚糖 抑菌材料 立體化學(xué)　出處：《北京化工大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：一直以來,細菌在材料表面的粘附、增殖與隨后形成的生物膜對人們的健康構(gòu)成了嚴(yán)重的威脅�，F(xiàn)有的抗菌材料大多是通過固定或釋放殺菌劑來進行殺菌,在應(yīng)用方面還有一定的局限性�；诩毎梢宰R別材料表面不同的手性分子信號,我們設(shè)計通過共價鍵將手性龍腦這種藥物分子接枝到天然高分子(纖維素和殼聚糖)表面,用以在微生物接觸材料的初期就產(chǎn)生抑制細菌粘附的效果,防止生物膜的形成。由于材料主要是依靠其自身的表面立體化學(xué)結(jié)構(gòu)影響細菌識別表面分子信號來產(chǎn)生抑菌效果,因而推測不會促使細菌進化產(chǎn)生耐藥性。龍腦、纖維素、殼聚糖都是天然產(chǎn)物,接枝改性后的復(fù)合高分子無細胞毒性,它們也不會對環(huán)境造成污染。本文首次通過共價鍵將L-龍腦接枝到纖維素表面,從而得到龍腦接枝纖維素(BGC)抑菌材料,通過一系列的表征對高分子結(jié)構(gòu)進行了測定,證明BGC目標(biāo)產(chǎn)物成功合成,接枝率17%。微生物實驗研究表明,BGC材料對微生物有著良好的抗粘附和抑制生長的作用。主要的研究成果如下：1.BGC材料具有廣譜抑真菌性能,如總狀毛霉、黑曲霉、酵母等都不會在材料上粘附生長。2.BGC材料的抑菌時效可達8天以上,微觀形態(tài)學(xué)研究發(fā)現(xiàn)真菌的孢子在材料上呈現(xiàn)休眠狀態(tài)。3.BGC材料主要是應(yīng)用龍腦小分子的立體化學(xué)結(jié)構(gòu)在微生物粘附的初期產(chǎn)生抑菌作用。4.BGC材料具有良好的生物相容性,其細胞毒性與天然纖維素幾乎一致,是一種沒有生物毒性的材料,可用于醫(yī)用敷料、食品包裝等。同時,本文通過席夫堿鍵將L-龍腦接枝到羥乙基殼聚糖表面得到了一種龍腦功能化羥乙基殼聚糖(BFC)抑菌材料。微生物實驗研究表明,BFC材料在細菌數(shù)量少時呈現(xiàn)抗細菌粘附功效,當(dāng)細菌數(shù)量多時,呈現(xiàn)一定的殺菌功效。主要研究成果如下：1.BFC材料具有廣譜抑菌性能,如黑曲霉、大腸桿菌等都不會在材料上粘附生長。2.BFC材料對霉菌有著很強的抑菌性能,其抑菌時間可達到29天,微觀形態(tài)學(xué)研究發(fā)現(xiàn)霉菌在BFC材料表面不會萌發(fā),并且BFC材料表面會使細菌產(chǎn)生形變、死亡。3.通過BFC材料修飾導(dǎo)尿管的研究模型,我們發(fā)現(xiàn)BFC材料對大腸桿菌的生長抑制作用可達67 cell/cm2。4.BFC材料面對少量菌時主要呈現(xiàn)阻止微生物粘附生長的狀態(tài)；當(dāng)遭遇大量細菌時,席夫堿鍵發(fā)生斷裂,釋放部分龍腦小分子以及溶出一定量的羥乙基殼聚糖,這使得材料不僅具有阻止微生物粘附生長的作用,還對微生物有著良好的殺菌潛力。
[Abstract]:The adhesion, proliferation and subsequent biofilm formation of bacteria on the surface of materials have been a serious threat to human health. Most of the existing antimicrobial materials are used to sterilize by immobilization or release of fungicides. Because cells can recognize different chiral molecular signals on the surface of materials, we designed to graft chiral borneol molecules onto the surface of natural polymers (cellulose and chitosan) by covalent bonds. It is used to inhibit bacterial adhesion and prevent biofilm from forming in the early stage of microorganism contact material. Because the material mainly depends on its surface stereochemical structure to influence the bacteria recognition surface molecular signal to produce bacteriostasis effect. Therefore, it is speculated that bacteria will not evolve to develop resistance. Borneol, cellulose and chitosan are all natural products, and the modified composite polymer is not cytotoxic. L- borneol was grafted onto cellulose surface by covalent bond for the first time to obtain borneol graft cellulose BGC-) bacteriostasis material, and the macromolecule structure was determined by a series of characterization. It was proved that the target product of BGC was successfully synthesized and the grafting rate was 17%. The microbiological experiment showed that the BGC material had good anti-adhesion and inhibitory effect on microorganism. The main research results were as follows: 1. BGC material has broad spectrum fungicidal inhibition properties, such as total Mucor, the main results of which are as follows: 1. Aspergillus Niger, yeast and so on do not adhere to growth on the material. 2. The bacteriostatic effect of BGC material can reach more than 8 days. Micromorphological study showed that the spores of fungi were dormant on the material. 3. The biocompatibility of BGC material was good by using the stereochemical structure of small molecule of borneol to produce bacteriostatic effect at the initial stage of microbial adhesion. Its cytotoxicity is almost the same as natural cellulose, it is a non-biotoxic material, can be used in medical dressings, food packaging, etc. At the same time, In this paper, a borneol functionalized hydroxyethyl chitosan (BFCC) antibacterial material was obtained by grafting L- borneol onto the surface of hydroxyethyl chitosan by Schiff base bond. The main research results are as follows: 1. BFC materials have broad spectrum bacteriostatic properties, such as Aspergillus Niger and Escherichia coli do not adhere to the material growth. 2. BFC materials have strong bacteriostasis to mold. The bacteriostasis time was up to 29 days. The micromorphological study showed that the mold did not germinate on the surface of BFC, and the surface of BFC made bacteria deform. 3. The model of catheter modification by BFC. We found that BFC could inhibit the growth of Escherichia coli as much as 67 cell/cm2.4.BFC, and the Schiff base bond was broken when a large number of bacteria were encountered. The release of some small borneol molecules and the dissolution of a certain amount of hydroxyethyl chitosan make the materials not only prevent the growth of microbes adhesion but also have a good bactericidal potential.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R318.08

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本文編號：1537962