本文關(guān)鍵詞：納米銀對大腸桿菌的抗菌作用及其機制，由筆耕文化傳播整理發(fā)布。

納米銀對大腸桿菌的抗菌作用及其機制

Study of Antimicrobial Activity and Mechanism of Silver Nanoparticles on Escherichia coli

[1] [2] [3] [4] [5]

XIE Xiao-bao , LI Wen-ru , ZENG Hai-yan , OUYANG You-sheng , CHEN Yi-ben（1 Guangdong Institute of Microbiology, Guangzhou 510070,China; 2 Guangdong Provincial Key Laborator

[1]廣東省微生物研究所,廣州510070; [2]廣東省菌種保藏與應用重點實驗室,廣州510070

文章摘要：以大腸桿菌為研究對象，對納米銀的抗菌效果進行了研究，并對其抗菌機制做了初步探討。納米銀對大腸桿菌的抑制生長曲線的結(jié)果表明，20μg／mL的納米銀能夠完全抑制10^6cfu／mL的大腸桿菌細胞生長，納米銀使大腸桿菌的延滯期加長，并且納米銀濃度越高，延滯期越長。采用透射電鏡觀察了經(jīng)納米銀粒子處理過的大腸桿菌細胞形態(tài)變化過程，結(jié)果顯示納米銀粒子先在細胞壁上產(chǎn)生小的孔洞，通過這些孔洞進入周質(zhì)空間，導致細胞膜成分滲漏和破壞細胞膜，進而進入細胞內(nèi)部。進入細胞內(nèi)部的納米銀粒子使DNA濃縮呈緊張態(tài)，并與破損細菌的細胞質(zhì)結(jié)合積聚，最后引起胞內(nèi)物質(zhì)流失。另外，納米銀對大腸桿菌總DNA影響的分析表明，，隨著納米銀濃度的增高，大腸桿菌總DNA樣品降解的程度增大。

Abstr：The antimicrobial effect of the nanosilver on Escherichia coli and the antimicrobial mecha- nism was studied elementarily. Experimental results indicated that the silver nanoparticles of 20 μg/ mL could inhibit completely the growth of 10^6 efu/mL cells in liquid LB medium. The growth curves showed that silver nanoparticles prolonged the lag phase of E. coli, and the higher of the concentra- tion of silver nanopartieles, the longer of the lag phase of E. coli. Transmission electron microscopy （TEM） was used to evaluate the cell morphology of both the normal and the treated E. coli. The ob- servation with TEM suggested that silver nanoparticles lead to the formation of ＂pits＂ in cell wall of the bacteria, and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane. Then the silver nanoparticles could enter into the bacterial cell, which not only condensed DNA, but also combined and coagulated with the cytoplasm of damaged bacteria. Finally, silver nano- particles resulted in the leakage of cytoplasmic component. Moreover, the analysis of agar gel electro- phoresis demonstrated that silver nanoparticles could increase the decomposability of genome DNA.

文章關(guān)鍵詞：

Keyword：:nanosilver Escherichia coli antimicrobial activity

課題項目：廣東省自然科學基金資助項目（A06104481）

作者信息：會員可見

  本文關(guān)鍵詞：納米銀對大腸桿菌的抗菌作用及其機制，由筆耕文化傳播整理發(fā)布。