本文關(guān)鍵詞： 弱激光 LED 紅細(xì)胞 溶血 光敏化　出處：《南京理工大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：弱激光和LED光應(yīng)用于臨床照射治療,已取得多方面療效,受到很大重視,有重要的應(yīng)用前景。然而其治療機(jī)理尚未有明確認(rèn)識(shí),相關(guān)基礎(chǔ)研究有所不足,一些疑團(tuán)有待解決。 本文通過(guò)實(shí)驗(yàn)研究了低強(qiáng)度623nm、636nm LED光和405nm半導(dǎo)體激光照射兔離體紅細(xì)胞所產(chǎn)生的促溶血效應(yīng)。實(shí)驗(yàn)中,采集了實(shí)驗(yàn)兔的新鮮血液,抗凝處理,生理鹽水洗滌,配置成所需濃度的紅細(xì)胞混懸液,分別設(shè)置LED光和激光照射組和對(duì)照組,檢測(cè)和對(duì)比紅細(xì)胞溶血率的數(shù)值。結(jié)果表明,在一定條件下623nm和636 nm LED照射組的溶血率顯著高于對(duì)照組,且隨照射功率的增大而升高；405nm激光照射組溶血率與對(duì)照組無(wú)顯著差異。 本文還對(duì)比研究了405nm、532nm、632.8nm、660nm激光和532nm、623nm、636nmLED光照射后的紅細(xì)胞溶血率,探索了不同波長(zhǎng)對(duì)紅細(xì)胞溶血效應(yīng)的影響機(jī)理。對(duì)比結(jié)果表明：(1)紅光(623nm、632.8nm、636nm、660nm)對(duì)兔紅細(xì)胞有顯著的促溶血作用,而405nm紫光和532nm綠光沒(méi)有明顯的促溶血效應(yīng)；(2)紅光(623nm、632.8nm、636nm、660nm)中,以632.8nm激光的促溶血作用最強(qiáng),相同的照射功率下,血樣的溶血率最高；660nm激光在較低功率時(shí),促溶血的程度較低,但當(dāng)照射功率達(dá)30-40mW后,隨照射功率增大,促溶血的程度也明顯變大；(3)紅光照射產(chǎn)生的促溶血效應(yīng)都具有“延遲發(fā)生”的特點(diǎn)；(4)在紅光波段,不同波長(zhǎng)光的促溶血作用強(qiáng)度與原卟啉受激后在該波長(zhǎng)附近的熒光強(qiáng)度呈正相關(guān)關(guān)系。 通過(guò)分析上述實(shí)驗(yàn)結(jié)果和對(duì)比研究結(jié)果的生物學(xué)意義及其影響因素,提出了作者的觀點(diǎn),認(rèn)為紅細(xì)胞內(nèi)源性卟啉經(jīng)弱激光或LED光照射可引發(fā)光敏化反應(yīng),進(jìn)而導(dǎo)致系列生物學(xué)效應(yīng)。在弱激光和LED光照射治療過(guò)程中,這些效應(yīng)可起重要作用。紅細(xì)胞對(duì)紫光、綠光的吸收和對(duì)紅光的吸收機(jī)理不同,導(dǎo)致了紫光、綠光與紅光對(duì)紅細(xì)胞溶血影響的差異。 本文研究結(jié)果可為進(jìn)一步研究光與血液相互作用的機(jī)理,弱激光和LED光照射療法的治療機(jī)理,以及弱激光和LED光的生物效應(yīng)及機(jī)理,提供基礎(chǔ)研究依據(jù)和理論指導(dǎo)。
[Abstract]:The application of weak laser and LED light in clinical irradiation treatment has obtained many curative effects, has been paid great attention to, and has important application prospects. However, its therapeutic mechanism has not been clearly understood, and the related basic research is insufficient. Some doubts remain to be solved. In this paper, the hemolytic effect of low intensity 636nm LED light and 405nm semiconductor laser irradiation on rabbit erythrocytes in vitro was studied. The fresh blood, anticoagulant treatment, normal saline washing, red blood cell suspension of the required concentration were collected, and the LED light and laser irradiation group and the control group were set up respectively. The results showed that the hemolysis rate of 623nm and 636nm LED irradiation group was significantly higher than that of the control group under certain conditions, and increased with the increase of irradiation power. There was no significant difference in hemolysis rate between 405nm laser irradiation group and control group. The erythrocyte hemolysis rate of 405nmO5nmO532nmO632.8nm 660nm laser and 532nmC623nmP636nmLED was also studied in this paper. The effects of different wavelengths on erythrocyte hemolysis were investigated. 660nm) had significant hemolytic effect on rabbit red blood cells, but 405nm purple light and 532nm green light had no obvious hemolytic effect. (2) in 623 nm (623 nm), 636 nm (660 nm), 632.8 nm laser had the strongest hemolytic effect under the same irradiation power. The hemolysis rate of blood sample was the highest. The degree of hemolysis promoted by 660nm laser at low power was lower, but when the irradiation power reached 30-40MW, the degree of hemolysis was obviously increased with the increase of irradiation power. (3) the hemolytic effect of red light irradiation has the characteristic of "delayed occurrence"; (4) in the red light band, the intensity of hemolysis of different wavelengths of light is positively correlated with the fluorescence intensity of protoporphyrin in the vicinity of this wavelength. Based on the analysis of the biological significance of the above experimental results and the comparative study results and their influencing factors, this paper puts forward the author's view that red blood cell endogenous porphyrin irradiation with weak laser or LED light can induce Guang Min reaction. These effects may play an important role in the treatment of weak laser and LED light irradiation. The absorption mechanism of red blood cells to purple light, green light and red light is different, leading to purple light. Difference between green light and red light on erythrocyte hemolysis. The results can be used to further study the mechanism of the interaction between light and blood, the therapeutic mechanism of low laser and LED light irradiation, and the biological effect and mechanism of weak laser and LED light. To provide basic research basis and theoretical guidance.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.51

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