發(fā)布時(shí)間：2018-01-29 16:47

  本文關(guān)鍵詞： 鈦合金 表面形貌 細(xì)胞黏附 分形維數(shù)　出處：《哈爾濱工業(yè)大學(xué)》2012年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：鈦及鈦合金作為生物材料在醫(yī)學(xué)方面已經(jīng)得到了廣泛的應(yīng)用，特別是在整形外科、口腔外科及心血管外科等領(lǐng)域。鈦合金材料作為骨科植入物的理想材料，必需具有優(yōu)良的生物相容性，能夠與人體的內(nèi)環(huán)境形成良好的界面結(jié)合。生物材料的表面微觀形貌是影響機(jī)體與植入材料表面相互作用的主要因素。對(duì)植入物的表面結(jié)構(gòu)進(jìn)行圖案化設(shè)計(jì)可以提高生物材料在體內(nèi)的相容性，從而促進(jìn)骨整合作用。因此，對(duì)鈦系生物醫(yī)用材料的表面微觀形貌進(jìn)行全面的評(píng)價(jià)并開(kāi)展細(xì)胞黏附、增殖及生物學(xué)形態(tài)的研究具有重要意義。 本課題選用Ti6Al7Nb生物材料，通過(guò)砂紙拋光、機(jī)械磨削及噴砂處理獲得不同微觀形貌的表面，進(jìn)行MC3T3成骨細(xì)胞的黏附情況、增殖能力及形態(tài)學(xué)觀察研究。實(shí)驗(yàn)結(jié)果表明鈦合金的表面微觀形貌影響著成骨細(xì)胞的黏著、鋪展及分裂生長(zhǎng)，并且經(jīng)過(guò)分析總結(jié)得到不同表面粗糙度和分形維數(shù)對(duì)細(xì)胞黏附、增殖及形態(tài)的影響規(guī)律。 利用噻唑藍(lán)法（MTT）和細(xì)胞計(jì)數(shù)法分別檢測(cè)成骨細(xì)胞的黏附和增殖情況。從表面形貌的高度幅值角度指出：適合MC3T3細(xì)胞早期黏附的表面粗糙度為Ra=2.323±0.056μm、Rz=16.915±0.543μm；較光滑和較粗糙的表面均表現(xiàn)出偏低的細(xì)胞黏附率；光滑表面有利于成骨細(xì)胞的增殖。運(yùn)用分形理論對(duì)鈦合金表面形貌進(jìn)行分形維數(shù)計(jì)算，指出表面形貌的不規(guī)則程度隨分形維數(shù)的增加而增加。細(xì)胞實(shí)驗(yàn)結(jié)果表明：成骨細(xì)胞的黏附率和增殖率均隨分形維數(shù)的增加而降低；在相同時(shí)期，拋光表面較磨削表面和噴砂表面更適合成骨細(xì)胞的鋪展；隨著培養(yǎng)時(shí)間的增加，培養(yǎng)孔內(nèi)的成骨細(xì)胞由梭形間隙排列增殖為“鋪路狀”重疊生長(zhǎng)，拋光和磨削表面的細(xì)胞由不規(guī)則多邊形、長(zhǎng)梭形逐漸生長(zhǎng)為“鱗片狀”并由大量絲狀偽足相互連接匯合，細(xì)胞接觸引導(dǎo)現(xiàn)象明顯，，噴砂表面的細(xì)胞由條形逐漸趨于星射線狀，多指板狀偽足錨接于表面凹坑邊緣或是跨越溝槽與其他細(xì)胞相互相接。
[Abstract]:Titanium and titanium alloys as biomaterials have been widely used in medicine, especially in plastic surgery, oral surgery and cardiovascular surgery. Titanium alloy materials as orthopaedic implants are ideal materials. Good biocompatibility is required. The micro-morphology of biomaterials is the main factor affecting the interaction between the body and the implant surface. The surface structure of implants can be patterned. Improving the compatibility of biomaterials in vivo. Therefore, it is of great significance to evaluate the surface morphology of titanium biomedical materials and to study cell adhesion, proliferation and biological morphology. In this paper, Ti6Al7Nb biomaterials were used to obtain different microcosmic surfaces by sandpaper polishing, mechanical grinding and sandblasting. The adhesion of MC3T3 osteoblasts was carried out. The results showed that the surface morphology of titanium alloy affected the adhesion, spread and growth of osteoblasts. The effects of different surface roughness and fractal dimension on cell adhesion, proliferation and morphology were analyzed and summarized. Thiazolyl blue method. The adhesion and proliferation of osteoblasts were detected by cell counting method. The surface roughness suitable for early adhesion of MC3T3 cells was Ra=2.323 鹵0.056 渭 m. Rz=16.915 鹵0.543 渭 m; The smooth and rough surfaces showed low cell adhesion. Smooth surface is conducive to the proliferation of osteoblasts. Fractal theory is used to calculate the fractal dimension of titanium alloy surface morphology. It was pointed out that the irregular degree of surface morphology increased with the increase of fractal dimension. The results of cell experiment showed that the adhesion rate and proliferation rate of osteoblasts decreased with the increase of fractal dimension. In the same period, the polished surface is more suitable for osteoblast spreading than the grinding surface and the sand blasting surface. With the increase of culture time, the osteoblasts in the culture holes were arranged and proliferated from fusiform spaces to "paving" overlapping growth, and the cells on the polished and ground surfaces were irregular polygons. The long fusiform gradually grows into "scale" and is connected and conjuncted by a large number of filamentous pseudopodia, the cell contact and leading phenomenon is obvious, and the cells on the sand blasting surface gradually tend to star-ray shape from the stripe shape. Multiple-finger plate-shaped pseudopod anchors are attached to the edge of the surface pit or across grooves to connect with other cells.
【學(xué)位授予單位】：哈爾濱工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R318.08

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