本文關(guān)鍵詞： 膠原 模型 動物 骨再生 組織工程 生物材料 膜生物材料 復(fù)合膜 富血小板纖維蛋白 膠原膜 屏障膜 生長因子 引導(dǎo)骨再生 誘導(dǎo)骨再生　出處：《中國組織工程研究》2017年30期 　論文類型：期刊論文

【摘要】：背景:目前有部分關(guān)于富血小板纖維蛋白、膠原膜應(yīng)用于引導(dǎo)骨再生技術(shù)中促進骨缺損修復(fù)的研究,但將二者聯(lián)合使用與單純使用膠原膜或富血小板纖維蛋白膜修復(fù)骨缺損的對比研究尚未被報道。目的:比較富血小板纖維蛋白與膠原復(fù)合膜、單純富血小板纖維蛋白膜、單純膠原膜3種膜的引導(dǎo)骨再生能力。方法:取日本大耳兔24只,在顱頂骨建立3個骨缺損區(qū),分別植入復(fù)合膜(自體富血小板纖維蛋白膜與膠原膜)、單純自體富血小板纖維蛋白膜及單純膠原膜,植入后2,4,8,12周,進行顱頂骨缺損區(qū)X射線及組織學(xué)觀察。結(jié)果與結(jié)論:①X射線觀察:植入后2周:復(fù)合膜組缺損區(qū)見邊緣模糊的密度增高影;膠原膜組見極少量密度增高影像;富血小板纖維蛋白膜組邊緣區(qū)出現(xiàn)密度增高影像。植入后12周,復(fù)合膜組整個缺損區(qū)與周圍正常骨密度接近;膠原膜組環(huán)形密度增高影像區(qū)域較植入后8周擴大,但中心的密度較周邊正常骨組織密度低;富血小板纖維蛋白膜組缺損區(qū)可見個別部位的密度低于周圍正常骨;②組織學(xué)觀察:植入后2周,復(fù)合膜組見缺損區(qū)周圍出現(xiàn)纖維結(jié)締組織、新生毛細血管;富血小板纖維蛋白膜膜組、膠原膜組纖維結(jié)締組織和新生血管均少于復(fù)合膜組。植入后12周,復(fù)合膜組缺損區(qū)出現(xiàn)大量骨細胞,排列整齊,有骨小梁變粗變大,可見成熟骨組織形成,骨質(zhì)較好;富血小板纖維蛋白膜組可見骨細胞,骨小梁數(shù)目增多,但骨形成量較復(fù)合膜組少;膠原膜組可見少量新骨形成,有成骨細胞、破骨細胞,有明顯骨陷凹;③結(jié)果表明:富血小板纖維蛋白與膠原復(fù)合膜具有更好的成骨效果。
[Abstract]:Background: at present, there are some researches on platelet rich fibrin, collagen membrane used in guiding bone regeneration technology to promote bone defect repair. However, the comparative study of the combined use of collagen membrane and platelet rich fibrin membrane in repairing bone defect has not been reported. Objective: to compare the platelet rich fibrin and collagen composite membrane. The ability of guided bone regeneration by pure platelet-rich fibrin membrane and collagen membrane. Methods: three bone defect areas were established in the parietal bone of 24 Japanese big ear rabbits. The composite membrane (autogenous platelet-rich fibrin membrane and collagen membrane), autologous platelet-rich fibrin membrane and collagen membrane were implanted respectively. Results and conclusion X-ray observation: two weeks after implantation, the defect area of composite membrane group showed the density increased with blurry margin in the defect area of cranio-parietal bone. In collagenous membrane group, a few images of increased density were observed. The density increased in the peripheral area of the platelet-rich fibrin membrane group. 12 weeks after implantation, the whole defect area in the composite membrane group was close to the normal bone density. The density of the center of the collagen membrane group was lower than that of the surrounding normal bone tissue. In the platelet-rich fibrin membrane group, the density of some parts was lower than that of the surrounding normal bone. 2histological observation: at 2 weeks after implantation, fibrous connective tissue and new capillaries appeared around the defect area in the composite membrane group. The fibrous connective tissue and neovascularization in platelet-rich fibrin membrane group and collagen membrane group were less than those in composite membrane group. 12 weeks after implantation, a large number of bone cells appeared in the defect area of composite membrane group and arranged neatly. The bone trabeculae became thicker and bigger, and the mature bone tissue was formed, and the bone was better. Bone cells were found in platelet-rich fibrin membrane group, and the number of bone trabeculae was increased, but the amount of bone formation was less than that of composite membrane group. In collagen membrane group, a small amount of new bone was formed, including osteoblasts, osteoclasts and obvious concave bone. The results showed that platelet-rich fibrin / collagen composite membrane had better osteogenic effect.
【作者單位】： 西南醫(yī)科大學(xué)口腔醫(yī)學(xué)院;解放軍成都軍區(qū)機關(guān)醫(yī)院口腔科;新疆維吾爾自治區(qū)人民醫(yī)院口腔科;
【基金】：成都軍區(qū)機關(guān)醫(yī)院院內(nèi)科研項目(YG201601)~~
【分類號】：R318.08;R651
【正文快照】： 文章快速閱讀:復(fù)合膜修復(fù)兔顱頂骨缺損檢測指標(biāo):富血小板纖維蛋白膜+膠原膜結(jié)果表明:植入后2,4,實驗動物:8,12周,進富血小板纖維兔膠原膜行顱頂骨缺蛋白與膠原復(fù)損區(qū)X射線合膜具有更好及組織學(xué)觀的成骨效果。富血小板纖維蛋白膜察文題釋義:富血小板纖維蛋白:2000年由法國科學(xué)

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